Transient Aggregates in Protein Folding are Easily Mistaken for Folding Intermediates
(1997) In Proceedings of the National Academy of Sciences 94(12). p.6084-6086- Abstract
- It has been questioned recently whether populated intermediates are important for the protein folding process or are artefacts trapped in nonproductive pathways. We report here that the rapidly formed intermediate of the spliceosomal protein U1A is an off-pathway artefact caused by transient aggregation of denatured protein under native conditions. Transient aggregates are easily mistaken for structured monomers and could be a general problem in time-resolved folding studies.
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/126214
- author
- Silow, Maria and Oliveberg, Mikael LU
- organization
- publishing date
- 1997
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Biochemistry, U1A spliceosomal protein, Aggregation artefacts, Off-pathway intermediates
- in
- Proceedings of the National Academy of Sciences
- volume
- 94
- issue
- 12
- pages
- 6084 - 6086
- publisher
- National Academy of Sciences
- external identifiers
-
- scopus:0030958760
- ISSN
- 1091-6490
- DOI
- 10.1073/pnas.94.12.6084
- language
- English
- LU publication?
- yes
- id
- 1954137a-f31c-4aae-9ca7-3c055c4ccd46 (old id 126214)
- date added to LUP
- 2016-04-01 12:19:26
- date last changed
- 2022-01-27 02:01:47
@article{1954137a-f31c-4aae-9ca7-3c055c4ccd46, abstract = {{It has been questioned recently whether populated intermediates are important for the protein folding process or are artefacts trapped in nonproductive pathways. We report here that the rapidly formed intermediate of the spliceosomal protein U1A is an off-pathway artefact caused by transient aggregation of denatured protein under native conditions. Transient aggregates are easily mistaken for structured monomers and could be a general problem in time-resolved folding studies.}}, author = {{Silow, Maria and Oliveberg, Mikael}}, issn = {{1091-6490}}, keywords = {{Biochemistry; U1A spliceosomal protein; Aggregation artefacts; Off-pathway intermediates}}, language = {{eng}}, number = {{12}}, pages = {{6084--6086}}, publisher = {{National Academy of Sciences}}, series = {{Proceedings of the National Academy of Sciences}}, title = {{Transient Aggregates in Protein Folding are Easily Mistaken for Folding Intermediates}}, url = {{http://dx.doi.org/10.1073/pnas.94.12.6084}}, doi = {{10.1073/pnas.94.12.6084}}, volume = {{94}}, year = {{1997}}, }