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Crystallization and preliminary X-ray analysis of human dUTPase

Larsson, G ; Nyman, Per-Olof LU and Svensson, L A (1996) In Acta Crystallographica. Section D: Biological Crystallography D52(5). p.1039-1040
Abstract
Human dUTPase, expressed in Escherichia coli, has been crystallized. Single crystals were obtained by the vapour-diffusion technique using 2-propanol and PEG 4000 as precipitants. The enzyme was co-crystallized with the substrate dUTP and a metal chelator EDTA to prevent hydrolysis of the substrate. The crystals belong to the orthorombic space group P212121 with cell dimensions a = 67.51, b = 68.26 and c = 91.00 Å. The crystallographic asymmetric unit contains one trimer of identical subunits.
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author
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organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Acta Crystallographica. Section D: Biological Crystallography
volume
D52
issue
5
pages
1039 - 1040
publisher
John Wiley & Sons Inc.
external identifiers
  • scopus:0030513996
ISSN
1399-0047
DOI
10.1107/S0907444996003277
language
English
LU publication?
yes
id
0aa1f215-5b3c-4bf1-9041-e1aa1c2d2f1a (old id 126300)
date added to LUP
2016-04-01 16:27:18
date last changed
2022-01-28 19:50:27
@article{0aa1f215-5b3c-4bf1-9041-e1aa1c2d2f1a,
  abstract     = {{Human dUTPase, expressed in Escherichia coli, has been crystallized. Single crystals were obtained by the vapour-diffusion technique using 2-propanol and PEG 4000 as precipitants. The enzyme was co-crystallized with the substrate dUTP and a metal chelator EDTA to prevent hydrolysis of the substrate. The crystals belong to the orthorombic space group P212121 with cell dimensions a = 67.51, b = 68.26 and c = 91.00 Å. The crystallographic asymmetric unit contains one trimer of identical subunits.}},
  author       = {{Larsson, G and Nyman, Per-Olof and Svensson, L A}},
  issn         = {{1399-0047}},
  language     = {{eng}},
  number       = {{5}},
  pages        = {{1039--1040}},
  publisher    = {{John Wiley & Sons Inc.}},
  series       = {{Acta Crystallographica. Section D: Biological Crystallography}},
  title        = {{Crystallization and preliminary X-ray analysis of human dUTPase}},
  url          = {{http://dx.doi.org/10.1107/S0907444996003277}},
  doi          = {{10.1107/S0907444996003277}},
  volume       = {{D52}},
  year         = {{1996}},
}