Crystal structure of the Escherichia coli dUTPase in complex with a substrate analogue (dUDP)
(1996) In Nature Structural & Molecular Biology 3(6). p.532-538- Abstract
- We have determined the structure of the homotrimeric dUTPase from Escherichia coli, complexed with an inhibitor and substrate analogue, dUDP. Three molecules of dUDP are found symmetrically bound per trimer, each in a shallow cleft between adjacent subunits, interacting with evolutionary conserved residues. The interactions of the uracil ring and the deoxypentose with the protein are consistent with the high specificity of the enzyme with respect to these groups. The positions of the two phosphate groups and adjacent water molecules are discussed in relation to the mechanism and kinetics of catalysis. The role that dUTPase plays in DNA metabolism makes the enzyme a potential target for chemotherapeutic drugs: the results presented here... (More)
- We have determined the structure of the homotrimeric dUTPase from Escherichia coli, complexed with an inhibitor and substrate analogue, dUDP. Three molecules of dUDP are found symmetrically bound per trimer, each in a shallow cleft between adjacent subunits, interacting with evolutionary conserved residues. The interactions of the uracil ring and the deoxypentose with the protein are consistent with the high specificity of the enzyme with respect to these groups. The positions of the two phosphate groups and adjacent water molecules are discussed in relation to the mechanism and kinetics of catalysis. The role that dUTPase plays in DNA metabolism makes the enzyme a potential target for chemotherapeutic drugs: the results presented here will aid in the design and development of inhibitory compounds. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/126305
- author
- Larsson, Gunilla ; Svensson, L Anders and Nyman, Per-Olof LU
- organization
- publishing date
- 1996
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Nature Structural & Molecular Biology
- volume
- 3
- issue
- 6
- pages
- 532 - 538
- publisher
- Nature Publishing Group
- external identifiers
-
- scopus:0029882967
- ISSN
- 1545-9985
- DOI
- 10.1038/nsb0696-532
- language
- English
- LU publication?
- yes
- id
- 96686a3d-9a6f-4f6e-8c5e-b9832898bb28 (old id 126305)
- date added to LUP
- 2016-04-01 15:32:24
- date last changed
- 2022-01-28 05:49:07
@article{96686a3d-9a6f-4f6e-8c5e-b9832898bb28, abstract = {{We have determined the structure of the homotrimeric dUTPase from Escherichia coli, complexed with an inhibitor and substrate analogue, dUDP. Three molecules of dUDP are found symmetrically bound per trimer, each in a shallow cleft between adjacent subunits, interacting with evolutionary conserved residues. The interactions of the uracil ring and the deoxypentose with the protein are consistent with the high specificity of the enzyme with respect to these groups. The positions of the two phosphate groups and adjacent water molecules are discussed in relation to the mechanism and kinetics of catalysis. The role that dUTPase plays in DNA metabolism makes the enzyme a potential target for chemotherapeutic drugs: the results presented here will aid in the design and development of inhibitory compounds.}}, author = {{Larsson, Gunilla and Svensson, L Anders and Nyman, Per-Olof}}, issn = {{1545-9985}}, language = {{eng}}, number = {{6}}, pages = {{532--538}}, publisher = {{Nature Publishing Group}}, series = {{Nature Structural & Molecular Biology}}, title = {{Crystal structure of the Escherichia coli dUTPase in complex with a substrate analogue (dUDP)}}, url = {{http://dx.doi.org/10.1038/nsb0696-532}}, doi = {{10.1038/nsb0696-532}}, volume = {{3}}, year = {{1996}}, }