Presence of a novel form of phosphatidylinositol 4-kinase in rat liver
(1995) In FEBS Letters 361(2-3). p.282-286- Abstract
- Rat liver microsomes contain two distinct forms of PtdIns 4-kinase which were resolved by heparin-Sepharose chromatography. One enzyme was identified as the type II PtdIns kinase previously isolated from exocytotic vesicles. The other enzyme, however, was a novel PtdIns 4-kinase isoform with properties differing from any other PtdIns kinase so far characterized. Both kinases were recognized by a monoclonal antibody specific for type II PtdIns 4-kinase, but the novel enzyme was considerably less sensitive to inhibition by adenosine and Ca2+ than type II enzymes, and in addition was specifically inhibited by submillimolar concentrations of dithioerythritol. The presence of a novel PtdIns 4-kinase isoform in rat liver raises the question of... (More)
- Rat liver microsomes contain two distinct forms of PtdIns 4-kinase which were resolved by heparin-Sepharose chromatography. One enzyme was identified as the type II PtdIns kinase previously isolated from exocytotic vesicles. The other enzyme, however, was a novel PtdIns 4-kinase isoform with properties differing from any other PtdIns kinase so far characterized. Both kinases were recognized by a monoclonal antibody specific for type II PtdIns 4-kinase, but the novel enzyme was considerably less sensitive to inhibition by adenosine and Ca2+ than type II enzymes, and in addition was specifically inhibited by submillimolar concentrations of dithioerythritol. The presence of a novel PtdIns 4-kinase isoform in rat liver raises the question of whether this enzyme is unique for this organ or whether it has a more widespread distribution but so far has avoided detection. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/126521
- author
- Olsson, Henric ; Martinez Arias, Wilma LU ; Drøbak, Bjørn K and Jergil, Bengt LU
- organization
- publishing date
- 1995
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Signal transduction, Phosphoinositide, Phosphatidyl inositol 4-kinase, Rat liver
- in
- FEBS Letters
- volume
- 361
- issue
- 2-3
- pages
- 282 - 286
- publisher
- Wiley-Blackwell
- external identifiers
-
- scopus:0028933680
- ISSN
- 1873-3468
- DOI
- 10.1016/0014-5793(95)00194-E
- language
- English
- LU publication?
- yes
- id
- ba34c6d4-c8dd-4fc4-a717-3cafc6ab7e02 (old id 126521)
- date added to LUP
- 2016-04-01 16:53:39
- date last changed
- 2021-01-03 09:55:40
@article{ba34c6d4-c8dd-4fc4-a717-3cafc6ab7e02,
abstract = {{Rat liver microsomes contain two distinct forms of PtdIns 4-kinase which were resolved by heparin-Sepharose chromatography. One enzyme was identified as the type II PtdIns kinase previously isolated from exocytotic vesicles. The other enzyme, however, was a novel PtdIns 4-kinase isoform with properties differing from any other PtdIns kinase so far characterized. Both kinases were recognized by a monoclonal antibody specific for type II PtdIns 4-kinase, but the novel enzyme was considerably less sensitive to inhibition by adenosine and Ca2+ than type II enzymes, and in addition was specifically inhibited by submillimolar concentrations of dithioerythritol. The presence of a novel PtdIns 4-kinase isoform in rat liver raises the question of whether this enzyme is unique for this organ or whether it has a more widespread distribution but so far has avoided detection.}},
author = {{Olsson, Henric and Martinez Arias, Wilma and Drøbak, Bjørn K and Jergil, Bengt}},
issn = {{1873-3468}},
keywords = {{Signal transduction; Phosphoinositide; Phosphatidyl inositol 4-kinase; Rat liver}},
language = {{eng}},
number = {{2-3}},
pages = {{282--286}},
publisher = {{Wiley-Blackwell}},
series = {{FEBS Letters}},
title = {{Presence of a novel form of phosphatidylinositol 4-kinase in rat liver}},
url = {{http://dx.doi.org/10.1016/0014-5793(95)00194-E}},
doi = {{10.1016/0014-5793(95)00194-E}},
volume = {{361}},
year = {{1995}},
}