Staphylococcal enterotoxin H induces V alpha-specific expansion of T cells.
(2003) In Journal of Immunology 170(8). p.4148-4154- Abstract
- Staphylococcal enterotoxin H (SEH) is a bacterial superantigen secreted by Staphylococcus aureus. Superantigens are presented on the MHC class II and activate large amounts of T cells by cross-linking APC and T cells. In this study, RT-PCR was used to show that SEH stimulates human T cells via the V domain of TCR, in particular V10 (TRAV27), while no TCR V-specific expansion was seen. This is in sharp contrast to all other studied bacterial superantigens, which are highly specific for TCR V. It was further confirmed by flow cytometry that SEH stimulation does not alter the levels of certain TCR V. In a functional assay addressing cross-reactivity, V binding superantigens were found to form one group, whereas SEH has different properties... (More)
- Staphylococcal enterotoxin H (SEH) is a bacterial superantigen secreted by Staphylococcus aureus. Superantigens are presented on the MHC class II and activate large amounts of T cells by cross-linking APC and T cells. In this study, RT-PCR was used to show that SEH stimulates human T cells via the V domain of TCR, in particular V10 (TRAV27), while no TCR V-specific expansion was seen. This is in sharp contrast to all other studied bacterial superantigens, which are highly specific for TCR V. It was further confirmed by flow cytometry that SEH stimulation does not alter the levels of certain TCR V. In a functional assay addressing cross-reactivity, V binding superantigens were found to form one group, whereas SEH has different properties that fit well with V reactivity. As SEH binds on top of MHC class II, an interaction between MHC and TCR upon SEH binding is not likely. This concludes that the specific expansion of TCR V is not due to contacts between MHC and TCR, instead we suggest that SEH directly interacts with the TCR V domain. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/128521
- author
- Lindkvist, Karin LU ; Pettersson, Helen ; Skartved, Niels Jörgen ; Walse, Björn and Forsberg, Göran
- organization
- publishing date
- 2003
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Journal of Immunology
- volume
- 170
- issue
- 8
- pages
- 4148 - 4154
- publisher
- American Association of Immunologists
- external identifiers
-
- pmid:12682246
- wos:000182171100027
- ISSN
- 1550-6606
- language
- English
- LU publication?
- yes
- id
- 5fb52653-3d60-4247-8abf-3cb9e48f9fc8 (old id 128521)
- alternative location
- http://www.jimmunol.org/cgi/content/abstract/170/8/4148
- date added to LUP
- 2016-04-01 15:57:52
- date last changed
- 2018-11-21 20:37:42
@article{5fb52653-3d60-4247-8abf-3cb9e48f9fc8,
abstract = {{Staphylococcal enterotoxin H (SEH) is a bacterial superantigen secreted by Staphylococcus aureus. Superantigens are presented on the MHC class II and activate large amounts of T cells by cross-linking APC and T cells. In this study, RT-PCR was used to show that SEH stimulates human T cells via the V domain of TCR, in particular V10 (TRAV27), while no TCR V-specific expansion was seen. This is in sharp contrast to all other studied bacterial superantigens, which are highly specific for TCR V. It was further confirmed by flow cytometry that SEH stimulation does not alter the levels of certain TCR V. In a functional assay addressing cross-reactivity, V binding superantigens were found to form one group, whereas SEH has different properties that fit well with V reactivity. As SEH binds on top of MHC class II, an interaction between MHC and TCR upon SEH binding is not likely. This concludes that the specific expansion of TCR V is not due to contacts between MHC and TCR, instead we suggest that SEH directly interacts with the TCR V domain.}},
author = {{Lindkvist, Karin and Pettersson, Helen and Skartved, Niels Jörgen and Walse, Björn and Forsberg, Göran}},
issn = {{1550-6606}},
language = {{eng}},
number = {{8}},
pages = {{4148--4154}},
publisher = {{American Association of Immunologists}},
series = {{Journal of Immunology}},
title = {{Staphylococcal enterotoxin H induces V alpha-specific expansion of T cells.}},
url = {{http://www.jimmunol.org/cgi/content/abstract/170/8/4148}},
volume = {{170}},
year = {{2003}},
}