Characterization of graphite electrodes modified with laccase from Trametes versicolor and their use for bioelectrochemical monitoring of phenolic compounds in flow injection analysis
(2003) In Analytica Chimica Acta 487(1). p.3-14- Abstract
- Spectrographic graphite electrodes were modified through adsorption with laccase from Trametes versicolor. The laccase-modified graphite electrode was used as the working electrode in an amperometric flow-through cell for monitoring phenolic compounds in a single line flow injection system. The experimental conditions for bioelectrochemical determination of catechol were studied and optimized. The relative standard deviation of the biosensor for catechol (10 M, n=12) was 1.0% and the reproducibility for six laccase-modified graphite electrodes, prepared and used different days was about 11%. The optimal conditions for the biosensor operation were: 0.1 M citrate buffer solution ( at pH 5.0), flow rate of 0.51 ml min−1 and a working... (More)
- Spectrographic graphite electrodes were modified through adsorption with laccase from Trametes versicolor. The laccase-modified graphite electrode was used as the working electrode in an amperometric flow-through cell for monitoring phenolic compounds in a single line flow injection system. The experimental conditions for bioelectrochemical determination of catechol were studied and optimized. The relative standard deviation of the biosensor for catechol (10 M, n=12) was 1.0% and the reproducibility for six laccase-modified graphite electrodes, prepared and used different days was about 11%. The optimal conditions for the biosensor operation were: 0.1 M citrate buffer solution ( at pH 5.0), flow rate of 0.51 ml min−1 and a working potential of −50 mV versus Ag|AgCl. At these conditions the responses of the biosensor for various phenolic compounds were recorded and the sensor characteristics were calculated and compared with those known for biosensors based on laccase from Coriolus hirsutus, cellobiose dehydrogenase (CDH) from Phanerochaete chrysosporium and horseradish peroxidase (HRP). (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/128913
- author
- Haghighi, Behzad LU ; Gorton, Lo LU ; Ruzgas, Tautgirdas LU and Jönsson, L J
- organization
- publishing date
- 2003
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Phenols, Laccase, Amperometry, Flow injection, Biosensors
- in
- Analytica Chimica Acta
- volume
- 487
- issue
- 1
- pages
- 3 - 14
- publisher
- Elsevier
- external identifiers
-
- wos:000183777500002
- scopus:0037669001
- ISSN
- 1873-4324
- DOI
- 10.1016/S0003-2670(03)00077-1
- language
- English
- LU publication?
- yes
- additional info
- The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Analytical Chemistry (S/LTH) (011001004)
- id
- c2b0facb-ff51-480d-9a58-ef807a748b02 (old id 128913)
- date added to LUP
- 2016-04-01 15:31:22
- date last changed
- 2022-02-12 08:22:04
@article{c2b0facb-ff51-480d-9a58-ef807a748b02,
abstract = {{Spectrographic graphite electrodes were modified through adsorption with laccase from Trametes versicolor. The laccase-modified graphite electrode was used as the working electrode in an amperometric flow-through cell for monitoring phenolic compounds in a single line flow injection system. The experimental conditions for bioelectrochemical determination of catechol were studied and optimized. The relative standard deviation of the biosensor for catechol (10 M, n=12) was 1.0% and the reproducibility for six laccase-modified graphite electrodes, prepared and used different days was about 11%. The optimal conditions for the biosensor operation were: 0.1 M citrate buffer solution ( at pH 5.0), flow rate of 0.51 ml min−1 and a working potential of −50 mV versus Ag|AgCl. At these conditions the responses of the biosensor for various phenolic compounds were recorded and the sensor characteristics were calculated and compared with those known for biosensors based on laccase from Coriolus hirsutus, cellobiose dehydrogenase (CDH) from Phanerochaete chrysosporium and horseradish peroxidase (HRP).}},
author = {{Haghighi, Behzad and Gorton, Lo and Ruzgas, Tautgirdas and Jönsson, L J}},
issn = {{1873-4324}},
keywords = {{Phenols; Laccase; Amperometry; Flow injection; Biosensors}},
language = {{eng}},
number = {{1}},
pages = {{3--14}},
publisher = {{Elsevier}},
series = {{Analytica Chimica Acta}},
title = {{Characterization of graphite electrodes modified with laccase from Trametes versicolor and their use for bioelectrochemical monitoring of phenolic compounds in flow injection analysis}},
url = {{http://dx.doi.org/10.1016/S0003-2670(03)00077-1}},
doi = {{10.1016/S0003-2670(03)00077-1}},
volume = {{487}},
year = {{2003}},
}