A capillary-based amperometric flow immunoassay for 2,4,6-trichlorophenol
(2003) In Analytical and Bioanalytical Chemistry 375(1). p.125-132- Abstract
- This paper describes the development of two different capillary-based heterogeneous competitive flow immunoassay formats (capillary flow injection immunoassay (CFIIA) and capillary sequential injection immunoassay (CSIIA)) for the determination of 2,4,6-trichlorophenol (2,4,6-TCP). The assays are based on the competition between the analyte and an analyte derivative labelled with the enzyme #-galactosidase, for an anti-TCP antibody, followed by the injection of the mixture at equilibrium into a flow stream, where separation between the fractions bound and unbound to the antibody is performed in a glass capillary containing immobilised protein A. The antibody-tracer fraction retained inside the protein A capillary was measured by injection... (More)
- This paper describes the development of two different capillary-based heterogeneous competitive flow immunoassay formats (capillary flow injection immunoassay (CFIIA) and capillary sequential injection immunoassay (CSIIA)) for the determination of 2,4,6-trichlorophenol (2,4,6-TCP). The assays are based on the competition between the analyte and an analyte derivative labelled with the enzyme #-galactosidase, for an anti-TCP antibody, followed by the injection of the mixture at equilibrium into a flow stream, where separation between the fractions bound and unbound to the antibody is performed in a glass capillary containing immobilised protein A. The antibody-tracer fraction retained inside the protein A capillary was measured by injection of 4-aminophenyl-#-D-galactoside (4-APG), followed by amperometric detection of the enzymatically generated 4-aminophenol (4-AP), leading to a negative correlation between the signal and the analyte concentration. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/128960
- author
- Nistor, Catalin LU and Emnéus, Jenny LU
- organization
- publishing date
- 2003
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Analytical and Bioanalytical Chemistry
- volume
- 375
- issue
- 1
- pages
- 125 - 132
- publisher
- Springer
- external identifiers
-
- pmid:12520448
- wos:000180848800030
- scopus:0037268719
- ISSN
- 1618-2642
- DOI
- 10.1007/s00216-002-1631-1
- language
- English
- LU publication?
- yes
- additional info
- The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Analytical Chemistry (S/LTH) (011001004)
- id
- 89f9bdd4-2275-4fb1-aac5-77f3e3fc9708 (old id 128960)
- date added to LUP
- 2016-04-01 11:50:58
- date last changed
- 2024-07-02 13:20:56
@article{89f9bdd4-2275-4fb1-aac5-77f3e3fc9708, abstract = {{This paper describes the development of two different capillary-based heterogeneous competitive flow immunoassay formats (capillary flow injection immunoassay (CFIIA) and capillary sequential injection immunoassay (CSIIA)) for the determination of 2,4,6-trichlorophenol (2,4,6-TCP). The assays are based on the competition between the analyte and an analyte derivative labelled with the enzyme #-galactosidase, for an anti-TCP antibody, followed by the injection of the mixture at equilibrium into a flow stream, where separation between the fractions bound and unbound to the antibody is performed in a glass capillary containing immobilised protein A. The antibody-tracer fraction retained inside the protein A capillary was measured by injection of 4-aminophenyl-#-D-galactoside (4-APG), followed by amperometric detection of the enzymatically generated 4-aminophenol (4-AP), leading to a negative correlation between the signal and the analyte concentration.}}, author = {{Nistor, Catalin and Emnéus, Jenny}}, issn = {{1618-2642}}, language = {{eng}}, number = {{1}}, pages = {{125--132}}, publisher = {{Springer}}, series = {{Analytical and Bioanalytical Chemistry}}, title = {{A capillary-based amperometric flow immunoassay for 2,4,6-trichlorophenol}}, url = {{http://dx.doi.org/10.1007/s00216-002-1631-1}}, doi = {{10.1007/s00216-002-1631-1}}, volume = {{375}}, year = {{2003}}, }