Improved selectivity of microbial biosensor using membrane coating. Application to the analysis of ethanol during fermentation
(2003) In Biosensors & Bioelectronics 18(9). p.1125-1134- Abstract
- A ferricyanide mediated microbial biosensor for ethanol detection was prepared by surface modification of a glassy carbon electrode. The selectivity of the whole Gluconobacter oxydans cell biosensor for ethanol determination was greatly enhanced by the size exclusion effect of a cellulose acetate (CA) membrane. The use of a CA membrane increased the ethanol to glucose sensitivity ratio by a factor of 58.2 and even the ethanol to glycerol sensitivity ratio by a factor of 7.5 compared with the use of a dialysis membrane. The biosensor provides rapid and sensitive detection of ethanol with a limit of detection of 0.85 M (S/N=3). The selectivity of the biosensor toward alcohols was better compared to previously published enzyme biosensors... (More)
- A ferricyanide mediated microbial biosensor for ethanol detection was prepared by surface modification of a glassy carbon electrode. The selectivity of the whole Gluconobacter oxydans cell biosensor for ethanol determination was greatly enhanced by the size exclusion effect of a cellulose acetate (CA) membrane. The use of a CA membrane increased the ethanol to glucose sensitivity ratio by a factor of 58.2 and even the ethanol to glycerol sensitivity ratio by a factor of 7.5 compared with the use of a dialysis membrane. The biosensor provides rapid and sensitive detection of ethanol with a limit of detection of 0.85 M (S/N=3). The selectivity of the biosensor toward alcohols was better compared to previously published enzyme biosensors based on alcohol oxidase or alcohol dehydrogenases. The biosensor was successfully used in an off-line monitoring of ethanol during batch fermentation by immobilized Saccharomyces cerevisiae cells with an initial glucose concentration of 200 g l−1 (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/128999
- author
- Tkac, Jan LU ; Vostiar, Igor LU ; Gorton, Lo LU ; Gemeiner, P and Sturdik, E
- organization
- publishing date
- 2003
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Biosensors & Bioelectronics
- volume
- 18
- issue
- 9
- pages
- 1125 - 1134
- publisher
- Elsevier
- external identifiers
-
- wos:000183942800006
- pmid:12788555
- scopus:0038360817
- ISSN
- 1873-4235
- DOI
- 10.1016/S0956-5663(02)00244-0
- language
- English
- LU publication?
- yes
- additional info
- The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Analytical Chemistry (S/LTH) (011001004)
- id
- bf1454a3-2e2b-4566-a2d3-85f8ef23d18c (old id 128999)
- date added to LUP
- 2016-04-01 17:06:17
- date last changed
- 2022-04-23 02:42:41
@article{bf1454a3-2e2b-4566-a2d3-85f8ef23d18c, abstract = {{A ferricyanide mediated microbial biosensor for ethanol detection was prepared by surface modification of a glassy carbon electrode. The selectivity of the whole Gluconobacter oxydans cell biosensor for ethanol determination was greatly enhanced by the size exclusion effect of a cellulose acetate (CA) membrane. The use of a CA membrane increased the ethanol to glucose sensitivity ratio by a factor of 58.2 and even the ethanol to glycerol sensitivity ratio by a factor of 7.5 compared with the use of a dialysis membrane. The biosensor provides rapid and sensitive detection of ethanol with a limit of detection of 0.85 M (S/N=3). The selectivity of the biosensor toward alcohols was better compared to previously published enzyme biosensors based on alcohol oxidase or alcohol dehydrogenases. The biosensor was successfully used in an off-line monitoring of ethanol during batch fermentation by immobilized Saccharomyces cerevisiae cells with an initial glucose concentration of 200 g l−1}}, author = {{Tkac, Jan and Vostiar, Igor and Gorton, Lo and Gemeiner, P and Sturdik, E}}, issn = {{1873-4235}}, language = {{eng}}, number = {{9}}, pages = {{1125--1134}}, publisher = {{Elsevier}}, series = {{Biosensors & Bioelectronics}}, title = {{Improved selectivity of microbial biosensor using membrane coating. Application to the analysis of ethanol during fermentation}}, url = {{http://dx.doi.org/10.1016/S0956-5663(02)00244-0}}, doi = {{10.1016/S0956-5663(02)00244-0}}, volume = {{18}}, year = {{2003}}, }