Localization of granule proteins in human eosinophil bone marrow progenitors
(1997) In International Archives of Allergy and Immunology 114(2). p.8-130- Abstract
- Eosinophils have a characteristic content of cationic proteins, stored in core-containing specific granules and released at sites of inflammation; coreless granules (sometimes called primary) are present in eosinophil promyelocytes. In order to determine a possible relationship between the two granule subsets, immunoelectron-microscopic techniques were used to determine the presence and precise intragranular distribution of major basic protein (MBP), eosinophil cationic protein (ECP), eosinophil peroxidase (EPO), and arylsulfatase B of eosinophil granules, as well as the Charcot-Leyden crystal (CLC) protein, in eosinophil progenitors of the bone marrow. MBP, ECP, EPO, and arylsulfatase B were observed in both coreless and core-containing... (More)
- Eosinophils have a characteristic content of cationic proteins, stored in core-containing specific granules and released at sites of inflammation; coreless granules (sometimes called primary) are present in eosinophil promyelocytes. In order to determine a possible relationship between the two granule subsets, immunoelectron-microscopic techniques were used to determine the presence and precise intragranular distribution of major basic protein (MBP), eosinophil cationic protein (ECP), eosinophil peroxidase (EPO), and arylsulfatase B of eosinophil granules, as well as the Charcot-Leyden crystal (CLC) protein, in eosinophil progenitors of the bone marrow. MBP, ECP, EPO, and arylsulfatase B were observed in both coreless and core-containing (specific) granules. The difference in the distribution of MBP, having a uniform distribution in coreless granules and a crystalloid distribution in core-containing (specific) granules, could indicate a maturational process of a common organelle. CLC protein was distributed in the cytosol, in the euchromatin of the nuclei, but was also present in a rare granular compartment of both immature and mature eosinophils. The present findings suggest that coreless granules develop into core-containing specific granules. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1296414
- author
- Egesten, Arne LU ; Calafat, J ; Weller, P F ; Knol, E F ; Janssen, H ; Walz, T M and Olsson, I
- organization
- publishing date
- 1997
- type
- Contribution to journal
- publication status
- published
- subject
- in
- International Archives of Allergy and Immunology
- volume
- 114
- issue
- 2
- pages
- 8 - 130
- publisher
- Karger
- external identifiers
-
- scopus:0030826321
- ISSN
- 1423-0097
- language
- English
- LU publication?
- yes
- id
- f5ba5676-26a6-4dae-8c65-775685062312 (old id 1296414)
- alternative location
- http://www.karger.com/Article/PDF/237657
- http://www.ncbi.nlm.nih.gov/pubmed/9338606
- date added to LUP
- 2016-04-04 13:52:29
- date last changed
- 2022-01-30 01:01:59
@article{f5ba5676-26a6-4dae-8c65-775685062312,
abstract = {{Eosinophils have a characteristic content of cationic proteins, stored in core-containing specific granules and released at sites of inflammation; coreless granules (sometimes called primary) are present in eosinophil promyelocytes. In order to determine a possible relationship between the two granule subsets, immunoelectron-microscopic techniques were used to determine the presence and precise intragranular distribution of major basic protein (MBP), eosinophil cationic protein (ECP), eosinophil peroxidase (EPO), and arylsulfatase B of eosinophil granules, as well as the Charcot-Leyden crystal (CLC) protein, in eosinophil progenitors of the bone marrow. MBP, ECP, EPO, and arylsulfatase B were observed in both coreless and core-containing (specific) granules. The difference in the distribution of MBP, having a uniform distribution in coreless granules and a crystalloid distribution in core-containing (specific) granules, could indicate a maturational process of a common organelle. CLC protein was distributed in the cytosol, in the euchromatin of the nuclei, but was also present in a rare granular compartment of both immature and mature eosinophils. The present findings suggest that coreless granules develop into core-containing specific granules.}},
author = {{Egesten, Arne and Calafat, J and Weller, P F and Knol, E F and Janssen, H and Walz, T M and Olsson, I}},
issn = {{1423-0097}},
language = {{eng}},
number = {{2}},
pages = {{8--130}},
publisher = {{Karger}},
series = {{International Archives of Allergy and Immunology}},
title = {{Localization of granule proteins in human eosinophil bone marrow progenitors}},
url = {{http://www.karger.com/Article/PDF/237657}},
volume = {{114}},
year = {{1997}},
}