High capacity xylose transport in Candida intermedia PYCC4715.
(2003) In FEMS Yeast Research 3(1). p.45-52- Abstract
- Xylose-utilising yeasts were screened to identify strains with high xylose transport capacity. Among the fastest-growing strains in xylose medium, Candida intermedia PYCC 4715 showed the highest xylose transport capacity. Maximal specific growth rate was the same in glucose and xylose media (small mu, Greekmax=0.5 h−1, 30°C). Xylose transport showed biphasic kinetics when cells were grown in either xylose- or glucose-limited culture. The high-affinity xylose/proton symport system (Km=0.2 mM, Vmax=7.5 mmol h−1 g−1) was more repressed by glucose than by xylose. The less specific low-affinity transport system (K=50 mM, Vmax=11 mmol h−1 g−1) appeared to operate through a facilitated-diffusion mechanism and was expressed constitutively.... (More)
- Xylose-utilising yeasts were screened to identify strains with high xylose transport capacity. Among the fastest-growing strains in xylose medium, Candida intermedia PYCC 4715 showed the highest xylose transport capacity. Maximal specific growth rate was the same in glucose and xylose media (small mu, Greekmax=0.5 h−1, 30°C). Xylose transport showed biphasic kinetics when cells were grown in either xylose- or glucose-limited culture. The high-affinity xylose/proton symport system (Km=0.2 mM, Vmax=7.5 mmol h−1 g−1) was more repressed by glucose than by xylose. The less specific low-affinity transport system (K=50 mM, Vmax=11 mmol h−1 g−1) appeared to operate through a facilitated-diffusion mechanism and was expressed constitutively. Inhibition experiments showed that glucose is a substrate of both xylose transport systems. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/132782
- author
- Gárdonyi, Márk LU ; Österberg, Måns ; Rodrigues, C ; Spencer-Martins, I and Hahn-Hägerdal, Bärbel LU
- organization
- publishing date
- 2003
- type
- Contribution to journal
- publication status
- published
- subject
- in
- FEMS Yeast Research
- volume
- 3
- issue
- 1
- pages
- 45 - 52
- publisher
- Oxford University Press
- external identifiers
-
- wos:000181502500007
- scopus:0037369565
- ISSN
- 1567-1364
- DOI
- 10.1016/S1567-1356(02)00137-X
- language
- English
- LU publication?
- yes
- id
- 8c48c7f9-5e14-4ff0-b45d-825a3560e4ba (old id 132782)
- date added to LUP
- 2016-04-01 11:52:55
- date last changed
- 2022-01-26 19:37:13
@article{8c48c7f9-5e14-4ff0-b45d-825a3560e4ba, abstract = {{Xylose-utilising yeasts were screened to identify strains with high xylose transport capacity. Among the fastest-growing strains in xylose medium, Candida intermedia PYCC 4715 showed the highest xylose transport capacity. Maximal specific growth rate was the same in glucose and xylose media (small mu, Greekmax=0.5 h−1, 30°C). Xylose transport showed biphasic kinetics when cells were grown in either xylose- or glucose-limited culture. The high-affinity xylose/proton symport system (Km=0.2 mM, Vmax=7.5 mmol h−1 g−1) was more repressed by glucose than by xylose. The less specific low-affinity transport system (K=50 mM, Vmax=11 mmol h−1 g−1) appeared to operate through a facilitated-diffusion mechanism and was expressed constitutively. Inhibition experiments showed that glucose is a substrate of both xylose transport systems.}}, author = {{Gárdonyi, Márk and Österberg, Måns and Rodrigues, C and Spencer-Martins, I and Hahn-Hägerdal, Bärbel}}, issn = {{1567-1364}}, language = {{eng}}, number = {{1}}, pages = {{45--52}}, publisher = {{Oxford University Press}}, series = {{FEMS Yeast Research}}, title = {{High capacity xylose transport in Candida intermedia PYCC4715.}}, url = {{http://dx.doi.org/10.1016/S1567-1356(02)00137-X}}, doi = {{10.1016/S1567-1356(02)00137-X}}, volume = {{3}}, year = {{2003}}, }