Xylose isomerase activity influences xylose fermentation with recombinant Saccharomyces cerevisiae strains expressing mutated xylA from Thermus thermophilus.
(2003) In Enzyme and Microbial Technology 32(5). p.567-573- Abstract
- Three xylose isomerase enzymes (XI) [Eur. J. Biochem. 269 (2002) 157], encoded by mutated xylA genes from Thermus thermophilus, were produced at two different levels in Saccharomyces cerevisiae; xylA genes were chromosomally integrated and expressed from multicopy plasmids, respectively. An extra copy of the endogenous xylulokinase gene (XKS1) was chromosomally integrated and the aldose reductase (AR) GRE3 gene was deleted. Ethanol was formed from xylose only when xylA was expressed from multicopy plasmids and when the specific XI activity was higher than 30 mU/mg protein. Deletion of the GRE3 gene was crucial for ethanol formation, possibly because reduced xylitol formation caused less inhibition of XI.
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https://lup.lub.lu.se/record/132840
- author
- Träff, Karin LU ; Lönn, Anna LU ; Otero Cordera, R R ; van Zyl,, W H and Hahn-Hägerdal, Bärbel LU
- organization
- publishing date
- 2003
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Xylose fermentation, Xylose, Xylose isomerase, Xylulokinase, Saccharomyces cerevisiae, GRE3
- in
- Enzyme and Microbial Technology
- volume
- 32
- issue
- 5
- pages
- 567 - 573
- publisher
- Elsevier
- external identifiers
-
- wos:000181908500010
- scopus:0242669383
- ISSN
- 0141-0229
- DOI
- 10.1016/S0141-0229(03)00024-3
- language
- English
- LU publication?
- yes
- id
- 5dc0b9e7-4d07-4ad8-9b77-34b183c67d8a (old id 132840)
- date added to LUP
- 2016-04-01 11:54:50
- date last changed
- 2022-03-28 17:33:46
@article{5dc0b9e7-4d07-4ad8-9b77-34b183c67d8a, abstract = {{Three xylose isomerase enzymes (XI) [Eur. J. Biochem. 269 (2002) 157], encoded by mutated xylA genes from Thermus thermophilus, were produced at two different levels in Saccharomyces cerevisiae; xylA genes were chromosomally integrated and expressed from multicopy plasmids, respectively. An extra copy of the endogenous xylulokinase gene (XKS1) was chromosomally integrated and the aldose reductase (AR) GRE3 gene was deleted. Ethanol was formed from xylose only when xylA was expressed from multicopy plasmids and when the specific XI activity was higher than 30 mU/mg protein. Deletion of the GRE3 gene was crucial for ethanol formation, possibly because reduced xylitol formation caused less inhibition of XI.}}, author = {{Träff, Karin and Lönn, Anna and Otero Cordera, R R and van Zyl,, W H and Hahn-Hägerdal, Bärbel}}, issn = {{0141-0229}}, keywords = {{Xylose fermentation; Xylose; Xylose isomerase; Xylulokinase; Saccharomyces cerevisiae; GRE3}}, language = {{eng}}, number = {{5}}, pages = {{567--573}}, publisher = {{Elsevier}}, series = {{Enzyme and Microbial Technology}}, title = {{Xylose isomerase activity influences xylose fermentation with recombinant Saccharomyces cerevisiae strains expressing mutated xylA from Thermus thermophilus.}}, url = {{http://dx.doi.org/10.1016/S0141-0229(03)00024-3}}, doi = {{10.1016/S0141-0229(03)00024-3}}, volume = {{32}}, year = {{2003}}, }