Methods to Detect Loss of Lysosomal Membrane Integrity
(2019) In Methods in Molecular Biology 1880. p.315-329- Abstract
- Loss of lysosomal membrane integrity, often referred to as lysosomal membrane permeabilization (LMP), occurs in many instances of cell death either as an initiating or as an amplifying event. Currently, the best method for detecting LMP is the galectin puncta formation assay which can be used for a broad range of sample types, both fixed and live, is easy to perform, and highly sensitive. This method, which is similar to the widely used LC3 puncta formation assay for autophagy, is based on the translocation of galectins to damaged lysosomes resulting in a change from uniform to punctate staining pattern. Here, we provide protocols for the galectin puncta formation assay in fixed and live cells and for an alternative assay based on... (More)
- Loss of lysosomal membrane integrity, often referred to as lysosomal membrane permeabilization (LMP), occurs in many instances of cell death either as an initiating or as an amplifying event. Currently, the best method for detecting LMP is the galectin puncta formation assay which can be used for a broad range of sample types, both fixed and live, is easy to perform, and highly sensitive. This method, which is similar to the widely used LC3 puncta formation assay for autophagy, is based on the translocation of galectins to damaged lysosomes resulting in a change from uniform to punctate staining pattern. Here, we provide protocols for the galectin puncta formation assay in fixed and live cells and for an alternative assay based on fluorescent dextran release from damaged lysosomes, which can be performed in parallel (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/137a5acd-52dd-4ac5-b0be-a2c94767f2a5
- author
- Aits, Sonja LU
- organization
- publishing date
- 2019-01
- type
- Chapter in Book/Report/Conference proceeding
- publication status
- published
- subject
- host publication
- Autophagy : Methods and Protocols - Methods and Protocols
- series title
- Methods in Molecular Biology
- editor
- Ktistakis, Nicholas and Florey, Oliver
- volume
- 1880
- pages
- 315 - 329
- publisher
- Humana Press
- external identifiers
-
- scopus:85059494657
- ISSN
- 1064-3745
- ISBN
- 978149398873-0
- DOI
- 10.1007/978-1-4939-8873-0
- project
- Understanding and therapeutic targeting of lysosome-dependent cell death
- Lysosomes in cell death - from molecular mechanisms to new treatment strategies
- language
- English
- LU publication?
- yes
- id
- 137a5acd-52dd-4ac5-b0be-a2c94767f2a5
- date added to LUP
- 2019-01-09 12:42:26
- date last changed
- 2022-12-15 06:35:27
@inbook{137a5acd-52dd-4ac5-b0be-a2c94767f2a5, abstract = {{Loss of lysosomal membrane integrity, often referred to as lysosomal membrane permeabilization (LMP), occurs in many instances of cell death either as an initiating or as an amplifying event. Currently, the best method for detecting LMP is the galectin puncta formation assay which can be used for a broad range of sample types, both fixed and live, is easy to perform, and highly sensitive. This method, which is similar to the widely used LC3 puncta formation assay for autophagy, is based on the translocation of galectins to damaged lysosomes resulting in a change from uniform to punctate staining pattern. Here, we provide protocols for the galectin puncta formation assay in fixed and live cells and for an alternative assay based on fluorescent dextran release from damaged lysosomes, which can be performed in parallel}}, author = {{Aits, Sonja}}, booktitle = {{Autophagy : Methods and Protocols}}, editor = {{Ktistakis, Nicholas and Florey, Oliver}}, isbn = {{978149398873-0}}, issn = {{1064-3745}}, language = {{eng}}, pages = {{315--329}}, publisher = {{Humana Press}}, series = {{Methods in Molecular Biology}}, title = {{Methods to Detect Loss of Lysosomal Membrane Integrity}}, url = {{http://dx.doi.org/10.1007/978-1-4939-8873-0}}, doi = {{10.1007/978-1-4939-8873-0}}, volume = {{1880}}, year = {{2019}}, }