Development of a laccase-modified electrode for amperometric detection of mono- and diphenols. The influence of enzyme storage method
(2004) In Analytical Letters 37(8). p.1497-1513- Abstract
- Results are reported for biosensors based on two different preparations of the same enzyme, laccase from Cerrena unicolor, one lyophilized and one stored frozen at - 18degreesC, for monitoring phenolic compounds. The enzyme was adsorbed on graphite electrodes and these were used in a flow through wall jet cell connected to a flow injection set-up. The electrodes were used at - 50 mV vs. AgAgCl. The effect of PH, flow rate of the carrier buffer was investigated as well as the operational and storage stability. Biosensors based on the frozen enzyme preparation were shown to be superior for biosensor construction.
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/138476
- author
- Jarosz-Wilkolazka, A ; Janusz, G ; Ruzgas, Tautgirdas LU ; Gorton, Lo LU ; Malarczyk, E and Leonowicz, A
- organization
- publishing date
- 2004
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Analytical Letters
- volume
- 37
- issue
- 8
- pages
- 1497 - 1513
- publisher
- Taylor & Francis
- external identifiers
-
- wos:000222755700004
- scopus:3142703597
- ISSN
- 0003-2719
- DOI
- 10.1081/AL-120037583
- language
- English
- LU publication?
- yes
- additional info
- The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Analytical Chemistry (S/LTH) (011001004)
- id
- 555be7d6-c2ac-46dd-a2fa-dc6f2e198eaa (old id 138476)
- date added to LUP
- 2016-04-01 16:10:27
- date last changed
- 2022-01-28 17:50:00
@article{555be7d6-c2ac-46dd-a2fa-dc6f2e198eaa, abstract = {{Results are reported for biosensors based on two different preparations of the same enzyme, laccase from Cerrena unicolor, one lyophilized and one stored frozen at - 18degreesC, for monitoring phenolic compounds. The enzyme was adsorbed on graphite electrodes and these were used in a flow through wall jet cell connected to a flow injection set-up. The electrodes were used at - 50 mV vs. AgAgCl. The effect of PH, flow rate of the carrier buffer was investigated as well as the operational and storage stability. Biosensors based on the frozen enzyme preparation were shown to be superior for biosensor construction.}}, author = {{Jarosz-Wilkolazka, A and Janusz, G and Ruzgas, Tautgirdas and Gorton, Lo and Malarczyk, E and Leonowicz, A}}, issn = {{0003-2719}}, language = {{eng}}, number = {{8}}, pages = {{1497--1513}}, publisher = {{Taylor & Francis}}, series = {{Analytical Letters}}, title = {{Development of a laccase-modified electrode for amperometric detection of mono- and diphenols. The influence of enzyme storage method}}, url = {{http://dx.doi.org/10.1081/AL-120037583}}, doi = {{10.1081/AL-120037583}}, volume = {{37}}, year = {{2004}}, }