Electrical "wiring" of viable Gluconobacter oxydans cells with a flexible osmium-redox polyelectrolyte
(2004) In Electrochemistry Communications 6(7). p.621-626- Abstract
- An application of a flexible polyvinylimidazole osmium functionalised polymer for efficient electrical wiring of whole Gluconobacter oxydans cells is described. The performance of the redox polymer as a mediator for intact bacterial cells was tested with glucose, ethanol and glycerol, typical substrates of the periplasmically localised PQQ-dependent membrane bound dehydrogenases. The efficiency of the electron transfer with the osmium redox polymer was compared with that of a soluble mediator (ferricyanide). Some basic bioelectrochemical characteristics, including I-max, K-m(app), inactivation constants and sensitivities were estimated for the mentioned substrates. The sensitivity profile for the tested substrates as well as the pH... (More)
- An application of a flexible polyvinylimidazole osmium functionalised polymer for efficient electrical wiring of whole Gluconobacter oxydans cells is described. The performance of the redox polymer as a mediator for intact bacterial cells was tested with glucose, ethanol and glycerol, typical substrates of the periplasmically localised PQQ-dependent membrane bound dehydrogenases. The efficiency of the electron transfer with the osmium redox polymer was compared with that of a soluble mediator (ferricyanide). Some basic bioelectrochemical characteristics, including I-max, K-m(app), inactivation constants and sensitivities were estimated for the mentioned substrates. The sensitivity profile for the tested substrates as well as the pH dependencies correspond well with the sensitivity profile for ferricyanide as soluble mediator. Significant stability differences for the substrates were found. (C) 2004 Elsevier B.V. All rights reserved. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/138484
- author
- Vostiar, Igor LU ; Ferapontova, Elena LU and Gorton, Lo LU
- organization
- publishing date
- 2004
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Pyrroloquinoline quinone, Mediated electron transfer, Gluconobacter
- in
- Electrochemistry Communications
- volume
- 6
- issue
- 7
- pages
- 621 - 626
- publisher
- Elsevier
- external identifiers
-
- wos:000222726100001
- scopus:2942652392
- ISSN
- 1388-2481
- DOI
- 10.1016/j.elecom.2004.04.017
- language
- English
- LU publication?
- yes
- additional info
- The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Analytical Chemistry (S/LTH) (011001004)
- id
- 76e5624c-1f9d-4145-8072-66b362e80ff6 (old id 138484)
- date added to LUP
- 2016-04-01 12:09:44
- date last changed
- 2022-04-13 07:00:08
@article{76e5624c-1f9d-4145-8072-66b362e80ff6,
abstract = {{An application of a flexible polyvinylimidazole osmium functionalised polymer for efficient electrical wiring of whole Gluconobacter oxydans cells is described. The performance of the redox polymer as a mediator for intact bacterial cells was tested with glucose, ethanol and glycerol, typical substrates of the periplasmically localised PQQ-dependent membrane bound dehydrogenases. The efficiency of the electron transfer with the osmium redox polymer was compared with that of a soluble mediator (ferricyanide). Some basic bioelectrochemical characteristics, including I-max, K-m(app), inactivation constants and sensitivities were estimated for the mentioned substrates. The sensitivity profile for the tested substrates as well as the pH dependencies correspond well with the sensitivity profile for ferricyanide as soluble mediator. Significant stability differences for the substrates were found. (C) 2004 Elsevier B.V. All rights reserved.}},
author = {{Vostiar, Igor and Ferapontova, Elena and Gorton, Lo}},
issn = {{1388-2481}},
keywords = {{Pyrroloquinoline quinone; Mediated electron transfer; Gluconobacter}},
language = {{eng}},
number = {{7}},
pages = {{621--626}},
publisher = {{Elsevier}},
series = {{Electrochemistry Communications}},
title = {{Electrical "wiring" of viable Gluconobacter oxydans cells with a flexible osmium-redox polyelectrolyte}},
url = {{http://dx.doi.org/10.1016/j.elecom.2004.04.017}},
doi = {{10.1016/j.elecom.2004.04.017}},
volume = {{6}},
year = {{2004}},
}