Novel antibody specificities targeting glycoprotein B of cytomegalovirus identified by molecular library technology
Carlsson, Fredrika LU ; Persson, Jonas LU ; Moreau, Emmanuel ; Côté, Marie-Hélène ; Lamarre, Alain and Ohlin, Mats LU
(2009)
In New Biotechnology
25(6).
p.429-436
- Abstract
- Antibodies provide some protection against cytomegalovirus-mediated disease. All aspects of antibody recognition of important viral antigens are however not fully appreciated. Glycoprotein B (gB), a key protein in the viral membrane, participates in viral infection and it is a component of prototype vaccines. By using combinatorial antibody library and selection technology, novel antibody specificities targeting gB have now been isolated. We define a monoclonal antibody fragment able to recognize site I of antigenic domain (AD) 2, a poorly immunogenic epitope targeted by potent virus-neutralizing antibodies, in a way that is different from the binding of antibodies induced by infection but similar to those induced by vaccination. We also... (More)
- Antibodies provide some protection against cytomegalovirus-mediated disease. All aspects of antibody recognition of important viral antigens are however not fully appreciated. Glycoprotein B (gB), a key protein in the viral membrane, participates in viral infection and it is a component of prototype vaccines. By using combinatorial antibody library and selection technology, novel antibody specificities targeting gB have now been isolated. We define a monoclonal antibody fragment able to recognize site I of antigenic domain (AD) 2, a poorly immunogenic epitope targeted by potent virus-neutralizing antibodies, in a way that is different from the binding of antibodies induced by infection but similar to those induced by vaccination. We also describe the existence of a novel epitope overlapping site I of AD-2 and AD-1, the immunodominant epitope of gB. These specificities, derived by molecular engineering, will be useful for the future assessment of humoral immune responses against this opportunistic viral infection. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1394515
- author
- Carlsson, Fredrika
LU
; Persson, Jonas
LU
; Moreau, Emmanuel
; Côté, Marie-Hélène
; Lamarre, Alain
and Ohlin, Mats
LU
- organization
- publishing date
- 2009
- type
- Contribution to journal
- publication status
- published
- subject
- in
- New Biotechnology
- volume
- 25
- issue
- 6
- pages
- 429 - 436
- publisher
- Elsevier
- external identifiers
-
- wos:000270772100011
- scopus:70449532221
- pmid:19464399
- ISSN
- 1876-4347
- DOI
- 10.1016/j.nbt.2009.05.003
- language
- English
- LU publication?
- yes
- id
- 76b368e2-927f-443c-b936-30e655fc3203 (old id 1394515)
- date added to LUP
- 2016-04-01 12:03:42
- date last changed
- 2022-01-26 22:13:00
@article{76b368e2-927f-443c-b936-30e655fc3203,
abstract = {{Antibodies provide some protection against cytomegalovirus-mediated disease. All aspects of antibody recognition of important viral antigens are however not fully appreciated. Glycoprotein B (gB), a key protein in the viral membrane, participates in viral infection and it is a component of prototype vaccines. By using combinatorial antibody library and selection technology, novel antibody specificities targeting gB have now been isolated. We define a monoclonal antibody fragment able to recognize site I of antigenic domain (AD) 2, a poorly immunogenic epitope targeted by potent virus-neutralizing antibodies, in a way that is different from the binding of antibodies induced by infection but similar to those induced by vaccination. We also describe the existence of a novel epitope overlapping site I of AD-2 and AD-1, the immunodominant epitope of gB. These specificities, derived by molecular engineering, will be useful for the future assessment of humoral immune responses against this opportunistic viral infection.}},
author = {{Carlsson, Fredrika and Persson, Jonas and Moreau, Emmanuel and Côté, Marie-Hélène and Lamarre, Alain and Ohlin, Mats}},
issn = {{1876-4347}},
language = {{eng}},
number = {{6}},
pages = {{429--436}},
publisher = {{Elsevier}},
series = {{New Biotechnology}},
title = {{Novel antibody specificities targeting glycoprotein B of cytomegalovirus identified by molecular library technology}},
url = {{http://dx.doi.org/10.1016/j.nbt.2009.05.003}},
doi = {{10.1016/j.nbt.2009.05.003}},
volume = {{25}},
year = {{2009}},
}