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Temperature Induced DNA Compaction in a Nonionic Lamellar Phase

Gonzalez Perez, Alfredo LU ; Bulut, Sanja LU ; Olsson, Ulf LU and Lindman, Björn LU (2008) 9th International Conference on Colloid Chemistry, 1997 135. p.174-180
Abstract
A nonionic lamellar phase was prepared using C10E3 in buffer solution at pH = 7.6. A suitable T4DNA concentration around 5 wt % was immobilized in a lamellar phase with 40 wt % C10E3. The mixed system was investigated at two temperatures, 25 and 5 degrees C by using cryo-fracture TEM direct imaging, fluorescence microscopy and small-angle X-ray scattering. The surprising results where obtained showing that the DNA conformation can be tuned to compacted and extended state at 25 and 5 degrees C, respectively. Additionally, DNA is aligned with a preferential orientation in the direction of the flow by simply injecting the sample in a capillary.
Please use this url to cite or link to this publication:
author
; ; and
organization
publishing date
type
Chapter in Book/Report/Conference proceeding
publication status
published
subject
keywords
compaction, surfactant, DNA, decompaction, Mesh phase, Nonionic, C10E3, lamellar phase
host publication
Colloids for Nano- and Biotechnology (Progress in Colloid and Polymer Science)
volume
135
pages
174 - 180
publisher
Springer
conference name
9th International Conference on Colloid Chemistry, 1997
conference location
Siofok, Hungary
conference dates
1997-07-06 - 1997-07-12
external identifiers
  • wos:000264002200025
  • scopus:62449186768
ISSN
0340-255X
ISBN
978-3-540-85133-2
DOI
10.1007/2882_2008_106
language
English
LU publication?
yes
id
938e24bc-9a51-435f-aeb1-04b3f934f6fd (old id 1399355)
date added to LUP
2016-04-01 14:59:21
date last changed
2022-01-28 03:26:41
@inproceedings{938e24bc-9a51-435f-aeb1-04b3f934f6fd,
  abstract     = {{A nonionic lamellar phase was prepared using C10E3 in buffer solution at pH = 7.6. A suitable T4DNA concentration around 5 wt % was immobilized in a lamellar phase with 40 wt % C10E3. The mixed system was investigated at two temperatures, 25 and 5 degrees C by using cryo-fracture TEM direct imaging, fluorescence microscopy and small-angle X-ray scattering. The surprising results where obtained showing that the DNA conformation can be tuned to compacted and extended state at 25 and 5 degrees C, respectively. Additionally, DNA is aligned with a preferential orientation in the direction of the flow by simply injecting the sample in a capillary.}},
  author       = {{Gonzalez Perez, Alfredo and Bulut, Sanja and Olsson, Ulf and Lindman, Björn}},
  booktitle    = {{Colloids for Nano- and Biotechnology (Progress in Colloid and Polymer Science)}},
  isbn         = {{978-3-540-85133-2}},
  issn         = {{0340-255X}},
  keywords     = {{compaction; surfactant; DNA; decompaction; Mesh phase; Nonionic; C10E3; lamellar phase}},
  language     = {{eng}},
  pages        = {{174--180}},
  publisher    = {{Springer}},
  title        = {{Temperature Induced DNA Compaction in a Nonionic Lamellar Phase}},
  url          = {{http://dx.doi.org/10.1007/2882_2008_106}},
  doi          = {{10.1007/2882_2008_106}},
  volume       = {{135}},
  year         = {{2008}},
}