Starch hydrolysing Bacillus halodurans isolates from a Kenyan soda lake
(2004) In Biotechnology Letters 26(10). p.823-828- Abstract
- Fourteen obligate alkaliphilic and halotolerant bacterial isolates, exhibiting extracellular amylase activity at 55degreesC and pH 10, were isolated from hot springs around Lake Bogoria, Kenya. From 16S rDNA sequence analysis, nine isolates shared 100% identity with Bacillus halodurans strain DSM 497(T), while the rest shared 99% identity with alkaliphilic Bacillus species A-59. PCR of the intergenic spacer region between 16S and 23S rRNA genes (ISR-PCR) divided the isolates into two groups, while tDNA-PCR divided them into three groups. Bacillus halodurans DSM 497T had a different ISR pattern from the isolates, while it had a tDNA-PCR profile similar to the group that shared 99% identity with alkaliphilic Bacillus species A-59. All... (More)
- Fourteen obligate alkaliphilic and halotolerant bacterial isolates, exhibiting extracellular amylase activity at 55degreesC and pH 10, were isolated from hot springs around Lake Bogoria, Kenya. From 16S rDNA sequence analysis, nine isolates shared 100% identity with Bacillus halodurans strain DSM 497(T), while the rest shared 99% identity with alkaliphilic Bacillus species A-59. PCR of the intergenic spacer region between 16S and 23S rRNA genes (ISR-PCR) divided the isolates into two groups, while tDNA-PCR divided them into three groups. Bacillus halodurans DSM 497T had a different ISR pattern from the isolates, while it had a tDNA-PCR profile similar to the group that shared 99% identity with alkaliphilic Bacillus species A-59. All isolates hydrolysed soluble starch as well as amylose, amylopectin and pullulan. The amylase activity (1.2 - 1.8 U ml(-1)) in the culture broths had an optimum temperature of 55 - 65degreesC, was stimulated by 1 mM Ca2+, and was either partially (16 - 30%) or completely inhibited by 1 mM EDTA. Activity staining of the cell-free culture supernatant from the isolates revealed five alkaline active amylase bands. (Less)
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https://lup.lub.lu.se/record/140701
- author
- Hashim, Suhaila LU ; Delgado, Osvaldo LU ; Hatti-Kaul, Rajni LU ; Mulaa, Francis J and Mattiasson, Bo LU
- organization
- publishing date
- 2004
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Biotechnology Letters
- volume
- 26
- issue
- 10
- pages
- 823 - 828
- publisher
- Springer
- external identifiers
-
- pmid:15269555
- wos:000221101800010
- scopus:3342881954
- ISSN
- 1573-6776
- DOI
- 10.1023/B:BILE.0000025885.19910.d7
- language
- English
- LU publication?
- yes
- id
- cc7796ee-ca6a-4674-805c-1172baf68faf (old id 140701)
- date added to LUP
- 2016-04-01 16:44:29
- date last changed
- 2022-04-15 06:43:42
@article{cc7796ee-ca6a-4674-805c-1172baf68faf, abstract = {{Fourteen obligate alkaliphilic and halotolerant bacterial isolates, exhibiting extracellular amylase activity at 55degreesC and pH 10, were isolated from hot springs around Lake Bogoria, Kenya. From 16S rDNA sequence analysis, nine isolates shared 100% identity with Bacillus halodurans strain DSM 497(T), while the rest shared 99% identity with alkaliphilic Bacillus species A-59. PCR of the intergenic spacer region between 16S and 23S rRNA genes (ISR-PCR) divided the isolates into two groups, while tDNA-PCR divided them into three groups. Bacillus halodurans DSM 497T had a different ISR pattern from the isolates, while it had a tDNA-PCR profile similar to the group that shared 99% identity with alkaliphilic Bacillus species A-59. All isolates hydrolysed soluble starch as well as amylose, amylopectin and pullulan. The amylase activity (1.2 - 1.8 U ml(-1)) in the culture broths had an optimum temperature of 55 - 65degreesC, was stimulated by 1 mM Ca2+, and was either partially (16 - 30%) or completely inhibited by 1 mM EDTA. Activity staining of the cell-free culture supernatant from the isolates revealed five alkaline active amylase bands.}}, author = {{Hashim, Suhaila and Delgado, Osvaldo and Hatti-Kaul, Rajni and Mulaa, Francis J and Mattiasson, Bo}}, issn = {{1573-6776}}, language = {{eng}}, number = {{10}}, pages = {{823--828}}, publisher = {{Springer}}, series = {{Biotechnology Letters}}, title = {{Starch hydrolysing Bacillus halodurans isolates from a Kenyan soda lake}}, url = {{http://dx.doi.org/10.1023/B:BILE.0000025885.19910.d7}}, doi = {{10.1023/B:BILE.0000025885.19910.d7}}, volume = {{26}}, year = {{2004}}, }