DNA-lipid systems. An amphiphile self-assembly and polymer-surfactant perspective
(2002) Conference on Lipid and Polymer-Lipid Systems, 2002 120. p.52-63- Abstract
- The interaction between DNA and oppositely charged surfactants has been investigated by several techniques, like fluorescence microscopy, electron microscopy, phase diagram determination, and ellipsometry. The phase behaviour is more strongly associative than that in previously studied systems. A precipitate is formed for very low amounts of surfactant and DNA. DNA compaction is a general phenomenon in the presence of multivalent ions and positively charged surfaces; because of the high charge density there are strong attractive ion correlation effects. The interaction between DNA and catanionic mixtures (i.e., mixtures of cationic and anionic surfactants) was also investigated. We observed that DNA compacts and adsorbs onto the surface of... (More)
- The interaction between DNA and oppositely charged surfactants has been investigated by several techniques, like fluorescence microscopy, electron microscopy, phase diagram determination, and ellipsometry. The phase behaviour is more strongly associative than that in previously studied systems. A precipitate is formed for very low amounts of surfactant and DNA. DNA compaction is a general phenomenon in the presence of multivalent ions and positively charged surfaces; because of the high charge density there are strong attractive ion correlation effects. The interaction between DNA and catanionic mixtures (i.e., mixtures of cationic and anionic surfactants) was also investigated. We observed that DNA compacts and adsorbs onto the surface of positively charged vesicles and that the addition of anionic surfactant can release free DNA back into solution from a compact globular complex between DNA and cationic surfactant. Finally, we investigated DNA interactions with polycations, chitosans with different chain lengths, by fluorescence microscopy, in vivo transfections assays and cryogenic transmission electron microscopy. The general conclusion is that a chitosan effective in promoting compaction is also efficient in transfection. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1407220
- author
- Lindman, Björn LU ; Mel'nikov, S ; Mel'nikova, Y ; Nylander, Tommy LU ; Eskilsson, K ; Miguel, M ; Dias, R and Leal, C
- organization
- publishing date
- 2002
- type
- Chapter in Book/Report/Conference proceeding
- publication status
- published
- subject
- keywords
- phase, chitosan, catanionic mixtures, DNA, cationic surfactants, behaviour
- host publication
- Lipid and Polymer-Lipid Systems
- volume
- 120
- pages
- 52 - 63
- publisher
- Springer
- conference name
- Conference on Lipid and Polymer-Lipid Systems, 2002
- conference location
- Chia Laguna, Italy
- conference dates
- 2002-10-01 - 2002-10-02
- external identifiers
-
- wos:000177564900008
- scopus:0036954377
- ISSN
- 0340-255X
- ISBN
- 978-3-540-43001-8
- DOI
- 10.1007/3-540-45291-5_8
- language
- English
- LU publication?
- yes
- id
- 13bc757e-8307-43c5-adb7-73a02f4961d1 (old id 1407220)
- date added to LUP
- 2016-04-01 16:15:22
- date last changed
- 2022-01-28 18:23:11
@inproceedings{13bc757e-8307-43c5-adb7-73a02f4961d1, abstract = {{The interaction between DNA and oppositely charged surfactants has been investigated by several techniques, like fluorescence microscopy, electron microscopy, phase diagram determination, and ellipsometry. The phase behaviour is more strongly associative than that in previously studied systems. A precipitate is formed for very low amounts of surfactant and DNA. DNA compaction is a general phenomenon in the presence of multivalent ions and positively charged surfaces; because of the high charge density there are strong attractive ion correlation effects. The interaction between DNA and catanionic mixtures (i.e., mixtures of cationic and anionic surfactants) was also investigated. We observed that DNA compacts and adsorbs onto the surface of positively charged vesicles and that the addition of anionic surfactant can release free DNA back into solution from a compact globular complex between DNA and cationic surfactant. Finally, we investigated DNA interactions with polycations, chitosans with different chain lengths, by fluorescence microscopy, in vivo transfections assays and cryogenic transmission electron microscopy. The general conclusion is that a chitosan effective in promoting compaction is also efficient in transfection.}}, author = {{Lindman, Björn and Mel'nikov, S and Mel'nikova, Y and Nylander, Tommy and Eskilsson, K and Miguel, M and Dias, R and Leal, C}}, booktitle = {{Lipid and Polymer-Lipid Systems}}, isbn = {{978-3-540-43001-8}}, issn = {{0340-255X}}, keywords = {{phase; chitosan; catanionic mixtures; DNA; cationic surfactants; behaviour}}, language = {{eng}}, pages = {{52--63}}, publisher = {{Springer}}, title = {{DNA-lipid systems. An amphiphile self-assembly and polymer-surfactant perspective}}, url = {{http://dx.doi.org/10.1007/3-540-45291-5_8}}, doi = {{10.1007/3-540-45291-5_8}}, volume = {{120}}, year = {{2002}}, }