Complete high-density lipoproteins in nanoparticle corona.
(2009) In The FEBS Journal 276(12). p.3372-3381- Abstract
- In a biological environment, nanoparticles immediately become covered by an evolving corona of biomolecules, which gives a biological identity to the nanoparticle and determines its biological impact and fate. Previous efforts at describing the corona have concerned only its protein content. Here, for the first time, we show, using size exclusion chromatography, NMR, and pull-down experiments, that copolymer nanoparticles bind cholesterol, triglycerides and phospholipids from human plasma, and that the binding reaches saturation. The lipid and protein binding patterns correspond closely with the composition of high-density lipoprotein (HDL). By using fractionated lipoproteins, we show that HDL binds to copolymer nanoparticles with much... (More)
- In a biological environment, nanoparticles immediately become covered by an evolving corona of biomolecules, which gives a biological identity to the nanoparticle and determines its biological impact and fate. Previous efforts at describing the corona have concerned only its protein content. Here, for the first time, we show, using size exclusion chromatography, NMR, and pull-down experiments, that copolymer nanoparticles bind cholesterol, triglycerides and phospholipids from human plasma, and that the binding reaches saturation. The lipid and protein binding patterns correspond closely with the composition of high-density lipoprotein (HDL). By using fractionated lipoproteins, we show that HDL binds to copolymer nanoparticles with much higher specificity than other lipoproteins, probably mediated by apolipoprotein A-I. Together with the previously identified protein binding patterns in the corona, our results imply that copolymer nanoparticles bind complete HDL complexes, and may be recognized by living systems as HDL complexes, opening up these transport pathways to nanoparticles. Apolipoproteins have been identified as binding to many other nanoparticles, suggesting that lipid and lipoprotein binding is a general feature of nanoparticles under physiological conditions. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1412324
- author
- Hellstrand, Erik LU ; Lynch, Iseult ; Andersson, Astra LU ; Drakenberg, Torbjörn LU ; Dahlbäck, Björn LU ; Dawson, Kenneth A ; Linse, Sara LU and Cedervall, Tommy LU
- organization
- publishing date
- 2009
- type
- Contribution to journal
- publication status
- published
- subject
- in
- The FEBS Journal
- volume
- 276
- issue
- 12
- pages
- 3372 - 3381
- publisher
- Wiley-Blackwell
- external identifiers
-
- wos:000266267800017
- pmid:19438706
- scopus:66249116656
- pmid:19438706
- ISSN
- 1742-464X
- DOI
- 10.1111/j.1742-4658.2009.07062.x
- language
- English
- LU publication?
- yes
- id
- 8daf8d29-d5bd-481d-a671-7c49929830c2 (old id 1412324)
- date added to LUP
- 2016-04-01 12:19:03
- date last changed
- 2022-04-29 03:45:19
@article{8daf8d29-d5bd-481d-a671-7c49929830c2, abstract = {{In a biological environment, nanoparticles immediately become covered by an evolving corona of biomolecules, which gives a biological identity to the nanoparticle and determines its biological impact and fate. Previous efforts at describing the corona have concerned only its protein content. Here, for the first time, we show, using size exclusion chromatography, NMR, and pull-down experiments, that copolymer nanoparticles bind cholesterol, triglycerides and phospholipids from human plasma, and that the binding reaches saturation. The lipid and protein binding patterns correspond closely with the composition of high-density lipoprotein (HDL). By using fractionated lipoproteins, we show that HDL binds to copolymer nanoparticles with much higher specificity than other lipoproteins, probably mediated by apolipoprotein A-I. Together with the previously identified protein binding patterns in the corona, our results imply that copolymer nanoparticles bind complete HDL complexes, and may be recognized by living systems as HDL complexes, opening up these transport pathways to nanoparticles. Apolipoproteins have been identified as binding to many other nanoparticles, suggesting that lipid and lipoprotein binding is a general feature of nanoparticles under physiological conditions.}}, author = {{Hellstrand, Erik and Lynch, Iseult and Andersson, Astra and Drakenberg, Torbjörn and Dahlbäck, Björn and Dawson, Kenneth A and Linse, Sara and Cedervall, Tommy}}, issn = {{1742-464X}}, language = {{eng}}, number = {{12}}, pages = {{3372--3381}}, publisher = {{Wiley-Blackwell}}, series = {{The FEBS Journal}}, title = {{Complete high-density lipoproteins in nanoparticle corona.}}, url = {{http://dx.doi.org/10.1111/j.1742-4658.2009.07062.x}}, doi = {{10.1111/j.1742-4658.2009.07062.x}}, volume = {{276}}, year = {{2009}}, }