Monitoring of glucose and glutamate using enzyme microstructures and scanning electrochemical microscopy.
(2009) In Bioelectrochemistry 76. p.81-86- Abstract
- Glucose oxidase and glutamate oxidase lines, with typical width of 100 microm, were patterned on gold surfaces using a micro-dispensing system, by shooting 100 pl droplets of the corresponding enzyme solutions. The probe of a scanning electrochemical microscope (SECM) was then carefully positioned in the close proximity of the enzyme microstructure and poised to +600 mV vs. Ag/AgCl, KCl 0.1 M. The H(2)O(2), generated by the enzyme lines at different concentrations of glucose and glutamate in the surrounding solution, was sequentially monitored. Reproducible calibration curves for glucose and glutamate were obtained in one single experiment, proving that the combination of enzyme microstructures with SECM can provide a new way of achieving... (More)
- Glucose oxidase and glutamate oxidase lines, with typical width of 100 microm, were patterned on gold surfaces using a micro-dispensing system, by shooting 100 pl droplets of the corresponding enzyme solutions. The probe of a scanning electrochemical microscope (SECM) was then carefully positioned in the close proximity of the enzyme microstructure and poised to +600 mV vs. Ag/AgCl, KCl 0.1 M. The H(2)O(2), generated by the enzyme lines at different concentrations of glucose and glutamate in the surrounding solution, was sequentially monitored. Reproducible calibration curves for glucose and glutamate were obtained in one single experiment, proving that the combination of enzyme microstructures with SECM can provide a new way of achieving multianalyte detection. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1434296
- author
- Muresan, Laura LU ; Nistor, Mihaela LU ; Gáspár, Szilveszter ; Popescu, Ionel Cătălin and Csöregi, Elisabeth LU
- organization
- publishing date
- 2009
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Bioelectrochemistry
- volume
- 76
- pages
- 81 - 86
- publisher
- Elsevier
- external identifiers
-
- wos:000270014400014
- pmid:19520620
- scopus:68549094259
- pmid:19520620
- ISSN
- 1878-562X
- DOI
- 10.1016/j.bioelechem.2009.05.004
- language
- English
- LU publication?
- yes
- additional info
- The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Analytical Chemistry (S/LTH) (011001004)
- id
- 9e45df28-9c96-4b05-bb5f-533acbd174c6 (old id 1434296)
- alternative location
- http://www.ncbi.nlm.nih.gov/pubmed/19520620?dopt=Abstract
- date added to LUP
- 2016-04-01 12:25:30
- date last changed
- 2022-01-27 03:33:00
@article{9e45df28-9c96-4b05-bb5f-533acbd174c6,
abstract = {{Glucose oxidase and glutamate oxidase lines, with typical width of 100 microm, were patterned on gold surfaces using a micro-dispensing system, by shooting 100 pl droplets of the corresponding enzyme solutions. The probe of a scanning electrochemical microscope (SECM) was then carefully positioned in the close proximity of the enzyme microstructure and poised to +600 mV vs. Ag/AgCl, KCl 0.1 M. The H(2)O(2), generated by the enzyme lines at different concentrations of glucose and glutamate in the surrounding solution, was sequentially monitored. Reproducible calibration curves for glucose and glutamate were obtained in one single experiment, proving that the combination of enzyme microstructures with SECM can provide a new way of achieving multianalyte detection.}},
author = {{Muresan, Laura and Nistor, Mihaela and Gáspár, Szilveszter and Popescu, Ionel Cătălin and Csöregi, Elisabeth}},
issn = {{1878-562X}},
language = {{eng}},
pages = {{81--86}},
publisher = {{Elsevier}},
series = {{Bioelectrochemistry}},
title = {{Monitoring of glucose and glutamate using enzyme microstructures and scanning electrochemical microscopy.}},
url = {{http://dx.doi.org/10.1016/j.bioelechem.2009.05.004}},
doi = {{10.1016/j.bioelechem.2009.05.004}},
volume = {{76}},
year = {{2009}},
}