Filter-exchange PGSE NMR determination of cell membrane permeability.
(2009) In Journal of Magnetic Resonance 200. p.291-295- Abstract
- A new PGSE NMR sequence is introduced for measuring diffusive transport across the plasma membrane of living cells. A "diffusion filter" and a variable mixing time precedes a standard PGSE block for diffusion encoding of the NMR signal. The filter is a PGSE block optimized for selectively removing the magnetization of the extracellular water. With increasing mixing time the intra- and extracellular components approach their equilibrium fractional populations. The rate of exchange can be measured using only a few minutes of instrument time. Water exchange over the plasma membrane of starved yeast cells is studied in the temperature range +5 to +32 degrees C.
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1470185
- author
- Åslund, Ingrid LU ; Nowacka, Agnieszka LU ; Nilsson, Markus LU and Topgaard, Daniel LU
- organization
- publishing date
- 2009
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Journal of Magnetic Resonance
- volume
- 200
- pages
- 291 - 295
- publisher
- Academic Press
- external identifiers
-
- wos:000272260900016
- pmid:19647458
- scopus:69949132949
- pmid:19647458
- ISSN
- 1096-0856
- DOI
- 10.1016/j.jmr.2009.07.015
- language
- English
- LU publication?
- yes
- id
- 55c69fc1-6eb9-41cc-bb4b-dba3beb1682e (old id 1470185)
- date added to LUP
- 2016-04-01 13:35:31
- date last changed
- 2022-03-21 19:24:55
@article{55c69fc1-6eb9-41cc-bb4b-dba3beb1682e, abstract = {{A new PGSE NMR sequence is introduced for measuring diffusive transport across the plasma membrane of living cells. A "diffusion filter" and a variable mixing time precedes a standard PGSE block for diffusion encoding of the NMR signal. The filter is a PGSE block optimized for selectively removing the magnetization of the extracellular water. With increasing mixing time the intra- and extracellular components approach their equilibrium fractional populations. The rate of exchange can be measured using only a few minutes of instrument time. Water exchange over the plasma membrane of starved yeast cells is studied in the temperature range +5 to +32 degrees C.}}, author = {{Åslund, Ingrid and Nowacka, Agnieszka and Nilsson, Markus and Topgaard, Daniel}}, issn = {{1096-0856}}, language = {{eng}}, pages = {{291--295}}, publisher = {{Academic Press}}, series = {{Journal of Magnetic Resonance}}, title = {{Filter-exchange PGSE NMR determination of cell membrane permeability.}}, url = {{http://dx.doi.org/10.1016/j.jmr.2009.07.015}}, doi = {{10.1016/j.jmr.2009.07.015}}, volume = {{200}}, year = {{2009}}, }