Integrated selective enrichment target - a microtechnology platform for matrix-assisted laser desorption/ionization-mass spectrometry applied on protein biomarkers in prostate diseases
(2004) In Electrophoresis 25(21-22). p.3769-3777- Abstract
- The performance of a miniaturized sample processing platform for matrix-assisted laser desorption/ionization-mass spectrometry (MALDI-MS), manufactured by silicon microfabrication, called integrated selective enrichment target (ISET) technology was evaluated in a biological context. The ISET serves as both sample treatment device and MALDI-MS target, and contains an array of 96 perforated nanovials, which each can be filled with 40 nL of reversed-phase beads. This methodology minimizes the number of sample transfers and the total surface area available for undesired adsorption of the analytes in order to provide high-sensitivity analysis. ISET technology was successfully applied for characterization of proteins coisolated by affinity... (More)
- The performance of a miniaturized sample processing platform for matrix-assisted laser desorption/ionization-mass spectrometry (MALDI-MS), manufactured by silicon microfabrication, called integrated selective enrichment target (ISET) technology was evaluated in a biological context. The ISET serves as both sample treatment device and MALDI-MS target, and contains an array of 96 perforated nanovials, which each can be filled with 40 nL of reversed-phase beads. This methodology minimizes the number of sample transfers and the total surface area available for undesired adsorption of the analytes in order to provide high-sensitivity analysis. ISET technology was successfully applied for characterization of proteins coisolated by affinity chromatography of prostate-specific antigen (PSA) from human seminal fluid. The application of ISET sample preparation enabled multiple analyses to be performed on a limited sample volume, which resulted in the discovery that prolactin inducible protein (PIP) was coisolated from the samples. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/151176
- author
- Ekström, Simon LU ; Wallman, Lars LU ; Malm, Johan LU ; Becker, Charlotte LU ; Lilja, Hans LU ; Laurell, Thomas LU and Marko-Varga, György LU
- organization
- publishing date
- 2004
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Electrophoresis
- volume
- 25
- issue
- 21-22
- pages
- 3769 - 3777
- publisher
- John Wiley & Sons Inc.
- external identifiers
-
- wos:000225679000027
- pmid:15565686
- scopus:10944251786
- ISSN
- 0173-0835
- DOI
- 10.1002/elps.200406094
- language
- English
- LU publication?
- yes
- additional info
- The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Clinical Chemistry, Malmö (013016000), Biomedical Engineering (011200011), Analytical Chemistry (S/LTH) (011001004)
- id
- 05f2fc54-64e5-4602-be98-1f8c8e36e9e2 (old id 151176)
- alternative location
- http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=15565686&query_hl=15&itool=pubmed_docsum
- date added to LUP
- 2016-04-01 12:19:23
- date last changed
- 2022-01-27 02:01:45
@article{05f2fc54-64e5-4602-be98-1f8c8e36e9e2, abstract = {{The performance of a miniaturized sample processing platform for matrix-assisted laser desorption/ionization-mass spectrometry (MALDI-MS), manufactured by silicon microfabrication, called integrated selective enrichment target (ISET) technology was evaluated in a biological context. The ISET serves as both sample treatment device and MALDI-MS target, and contains an array of 96 perforated nanovials, which each can be filled with 40 nL of reversed-phase beads. This methodology minimizes the number of sample transfers and the total surface area available for undesired adsorption of the analytes in order to provide high-sensitivity analysis. ISET technology was successfully applied for characterization of proteins coisolated by affinity chromatography of prostate-specific antigen (PSA) from human seminal fluid. The application of ISET sample preparation enabled multiple analyses to be performed on a limited sample volume, which resulted in the discovery that prolactin inducible protein (PIP) was coisolated from the samples.}}, author = {{Ekström, Simon and Wallman, Lars and Malm, Johan and Becker, Charlotte and Lilja, Hans and Laurell, Thomas and Marko-Varga, György}}, issn = {{0173-0835}}, language = {{eng}}, number = {{21-22}}, pages = {{3769--3777}}, publisher = {{John Wiley & Sons Inc.}}, series = {{Electrophoresis}}, title = {{Integrated selective enrichment target - a microtechnology platform for matrix-assisted laser desorption/ionization-mass spectrometry applied on protein biomarkers in prostate diseases}}, url = {{http://dx.doi.org/10.1002/elps.200406094}}, doi = {{10.1002/elps.200406094}}, volume = {{25}}, year = {{2004}}, }