Cyclodextrins in DNA decompaction.
(2010) In Colloids and Surfaces B: Biointerfaces 76(1). p.20-27- Abstract
- Individual T4DNA molecules, previously compacted by using a cationic surfactant (cetyltrimethylammonium bromide, CTAB), were successfully decompacted by the addition of an appropriate concentration of either alpha-cyclodextrin or beta-cyclodextrin (alpha-CD and beta-CD, respectively) due to the formation of inclusion complexes with the surfactant. The process was shown to be a non first-order transition from globules to coils. Density and sound velocity measurements as well as steady state fluorescence spectroscopy have confirmed the approximate CD concentration at which the globule-to-coil transition occurs. Phase maps of the DNA-CTA-CD systems were produced and the CTAB concentration range at which decompaction can be achieved was... (More)
- Individual T4DNA molecules, previously compacted by using a cationic surfactant (cetyltrimethylammonium bromide, CTAB), were successfully decompacted by the addition of an appropriate concentration of either alpha-cyclodextrin or beta-cyclodextrin (alpha-CD and beta-CD, respectively) due to the formation of inclusion complexes with the surfactant. The process was shown to be a non first-order transition from globules to coils. Density and sound velocity measurements as well as steady state fluorescence spectroscopy have confirmed the approximate CD concentration at which the globule-to-coil transition occurs. Phase maps of the DNA-CTA-CD systems were produced and the CTAB concentration range at which decompaction can be achieved was determined. Evidences for DNA-CD interaction were found, however, its nature and influence on the decompaction process was not yet determined. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1512146
- author
- Gonzalez Perez, Alfredo LU ; Carlstedt, Jonas LU ; Dias, Rita LU and Lindman, Björn LU
- organization
- publishing date
- 2010
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- CTAB, DNA, Cyclodextrins, Compaction, Decompaction
- in
- Colloids and Surfaces B: Biointerfaces
- volume
- 76
- issue
- 1
- pages
- 20 - 27
- publisher
- Elsevier
- external identifiers
-
- wos:000274767300003
- pmid:19897344
- scopus:74149084443
- pmid:19897344
- ISSN
- 1873-4367
- DOI
- 10.1016/j.colsurfb.2009.10.001
- language
- English
- LU publication?
- yes
- id
- fae0ce8c-5422-4558-8aa7-20268928b25c (old id 1512146)
- alternative location
- http://www.sciencedirect.com/science/article/B6TFS-4XDD042-2/2/a998a34def4a8fa34c0ba419a3fc5a54
- date added to LUP
- 2016-04-01 10:27:22
- date last changed
- 2022-01-25 23:20:44
@article{fae0ce8c-5422-4558-8aa7-20268928b25c,
abstract = {{Individual T4DNA molecules, previously compacted by using a cationic surfactant (cetyltrimethylammonium bromide, CTAB), were successfully decompacted by the addition of an appropriate concentration of either alpha-cyclodextrin or beta-cyclodextrin (alpha-CD and beta-CD, respectively) due to the formation of inclusion complexes with the surfactant. The process was shown to be a non first-order transition from globules to coils. Density and sound velocity measurements as well as steady state fluorescence spectroscopy have confirmed the approximate CD concentration at which the globule-to-coil transition occurs. Phase maps of the DNA-CTA-CD systems were produced and the CTAB concentration range at which decompaction can be achieved was determined. Evidences for DNA-CD interaction were found, however, its nature and influence on the decompaction process was not yet determined.}},
author = {{Gonzalez Perez, Alfredo and Carlstedt, Jonas and Dias, Rita and Lindman, Björn}},
issn = {{1873-4367}},
keywords = {{CTAB; DNA; Cyclodextrins; Compaction; Decompaction}},
language = {{eng}},
number = {{1}},
pages = {{20--27}},
publisher = {{Elsevier}},
series = {{Colloids and Surfaces B: Biointerfaces}},
title = {{Cyclodextrins in DNA decompaction.}},
url = {{http://dx.doi.org/10.1016/j.colsurfb.2009.10.001}},
doi = {{10.1016/j.colsurfb.2009.10.001}},
volume = {{76}},
year = {{2010}},
}