Binding studies on substrate- and enantio-selective molecularly imprinted polymers
(1991) In Analytical Letters 24(7). p.1137-1145- Abstract
- A molecularly imprinted polymer was prepared using tert-butyloxycarbonyl-L-phenylalanine as the print molecule and methacrylic acid as the functional monomer. The bulk polymer obtained was ground, sieved, packed into a column and investigated in the HPLC-mode by frontal chromatography to determine the number of binding sites and dissociation constants for the enantiomers interacting with the polymer. The dissociation constant for the L-enantiomer of the print molecule was lower than for the D-enantiomer (6.3 mM and 8.1 mM, respectively). This means that the affinity for the L-enantiomer was higher than for the D-enantiomer. The number of binding sites in the polymer giving rise to these dissociation constants were determined to be 28 μmol... (More)
- A molecularly imprinted polymer was prepared using tert-butyloxycarbonyl-L-phenylalanine as the print molecule and methacrylic acid as the functional monomer. The bulk polymer obtained was ground, sieved, packed into a column and investigated in the HPLC-mode by frontal chromatography to determine the number of binding sites and dissociation constants for the enantiomers interacting with the polymer. The dissociation constant for the L-enantiomer of the print molecule was lower than for the D-enantiomer (6.3 mM and 8.1 mM, respectively). This means that the affinity for the L-enantiomer was higher than for the D-enantiomer. The number of binding sites in the polymer giving rise to these dissociation constants were determined to be 28 μmol per g dry polymer. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1516052
- author
- Kempe, Maria LU and Mosbach, Klaus LU
- organization
- publishing date
- 1991
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Analytical Letters
- volume
- 24
- issue
- 7
- pages
- 1137 - 1145
- publisher
- Taylor & Francis
- external identifiers
-
- scopus:0000665502
- ISSN
- 0003-2719
- DOI
- 10.1080/00032719108052959
- language
- English
- LU publication?
- yes
- id
- 197029d0-7438-4e4f-a4a3-1f197ff20c31 (old id 1516052)
- date added to LUP
- 2016-04-01 15:53:30
- date last changed
- 2021-08-29 05:18:41
@article{197029d0-7438-4e4f-a4a3-1f197ff20c31, abstract = {{A molecularly imprinted polymer was prepared using tert-butyloxycarbonyl-L-phenylalanine as the print molecule and methacrylic acid as the functional monomer. The bulk polymer obtained was ground, sieved, packed into a column and investigated in the HPLC-mode by frontal chromatography to determine the number of binding sites and dissociation constants for the enantiomers interacting with the polymer. The dissociation constant for the L-enantiomer of the print molecule was lower than for the D-enantiomer (6.3 mM and 8.1 mM, respectively). This means that the affinity for the L-enantiomer was higher than for the D-enantiomer. The number of binding sites in the polymer giving rise to these dissociation constants were determined to be 28 μmol per g dry polymer.}}, author = {{Kempe, Maria and Mosbach, Klaus}}, issn = {{0003-2719}}, language = {{eng}}, number = {{7}}, pages = {{1137--1145}}, publisher = {{Taylor & Francis}}, series = {{Analytical Letters}}, title = {{Binding studies on substrate- and enantio-selective molecularly imprinted polymers}}, url = {{http://dx.doi.org/10.1080/00032719108052959}}, doi = {{10.1080/00032719108052959}}, volume = {{24}}, year = {{1991}}, }