Ectoine Production by Halomonas boliviensis: Optimization Using Response Surface Methodology.
(2010) In Marine Biotechnology 12. p.586-593- Abstract
- Two cultivation steps were used for production of biomass and ectoine by Halomonas boliviensis, respectively. The optimization of some nutrient parameters in each step was investigated by using response surface methodology. Twenty and 12 experiments were performed to attain optimal conditions for biomass and ectoine production, respectively. The model predicted a maximum biomass concentration of 3.34 g/L on optimization of NH(4)Cl, K(2)HPO(4), and MgSO(4)*7H(2)O concentrations during the first cultivation, while a maximum ectoine concentration of 1.27 g/L was predicted on optimizing NaCl and monosodium glutamate concentrations in the second cultivation. The experimental values obtained (3.36 g biomass/L and 1.25 g ectoine/L) were in good... (More)
- Two cultivation steps were used for production of biomass and ectoine by Halomonas boliviensis, respectively. The optimization of some nutrient parameters in each step was investigated by using response surface methodology. Twenty and 12 experiments were performed to attain optimal conditions for biomass and ectoine production, respectively. The model predicted a maximum biomass concentration of 3.34 g/L on optimization of NH(4)Cl, K(2)HPO(4), and MgSO(4)*7H(2)O concentrations during the first cultivation, while a maximum ectoine concentration of 1.27 g/L was predicted on optimizing NaCl and monosodium glutamate concentrations in the second cultivation. The experimental values obtained (3.36 g biomass/L and 1.25 g ectoine/L) were in good agreement with the predicted values. The optimized conditions were also used for two-step 1.5-L fed-batch fermentations. In the first step, biomass concentration of 28.7 g/L was obtained while in the second step biomass concentration increased to 63 g/L. Ectoine concentration of 9.2 g/L was obtained, and the overall ectoine productivity was 6.3 g/L/day, being among the highest reported so far. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1524065
- author
- Doan Van, Thuoc LU ; Guzmán, Hector LU ; Thi-Hang, Mai and Hatti-Kaul, Rajni LU
- organization
- publishing date
- 2010
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Marine Biotechnology
- volume
- 12
- pages
- 586 - 593
- publisher
- Springer
- external identifiers
-
- wos:000282592900011
- pmid:19957094
- scopus:77957756253
- pmid:19957094
- ISSN
- 1436-2236
- DOI
- 10.1007/s10126-009-9246-6
- language
- English
- LU publication?
- yes
- id
- ae468024-67a4-486d-bf0a-308dc527d43d (old id 1524065)
- date added to LUP
- 2016-04-01 11:00:53
- date last changed
- 2022-01-26 04:42:43
@article{ae468024-67a4-486d-bf0a-308dc527d43d, abstract = {{Two cultivation steps were used for production of biomass and ectoine by Halomonas boliviensis, respectively. The optimization of some nutrient parameters in each step was investigated by using response surface methodology. Twenty and 12 experiments were performed to attain optimal conditions for biomass and ectoine production, respectively. The model predicted a maximum biomass concentration of 3.34 g/L on optimization of NH(4)Cl, K(2)HPO(4), and MgSO(4)*7H(2)O concentrations during the first cultivation, while a maximum ectoine concentration of 1.27 g/L was predicted on optimizing NaCl and monosodium glutamate concentrations in the second cultivation. The experimental values obtained (3.36 g biomass/L and 1.25 g ectoine/L) were in good agreement with the predicted values. The optimized conditions were also used for two-step 1.5-L fed-batch fermentations. In the first step, biomass concentration of 28.7 g/L was obtained while in the second step biomass concentration increased to 63 g/L. Ectoine concentration of 9.2 g/L was obtained, and the overall ectoine productivity was 6.3 g/L/day, being among the highest reported so far.}}, author = {{Doan Van, Thuoc and Guzmán, Hector and Thi-Hang, Mai and Hatti-Kaul, Rajni}}, issn = {{1436-2236}}, language = {{eng}}, pages = {{586--593}}, publisher = {{Springer}}, series = {{Marine Biotechnology}}, title = {{Ectoine Production by Halomonas boliviensis: Optimization Using Response Surface Methodology.}}, url = {{http://dx.doi.org/10.1007/s10126-009-9246-6}}, doi = {{10.1007/s10126-009-9246-6}}, volume = {{12}}, year = {{2010}}, }