Antioxidants rescue photoreceptors in rd1 mice: Relationship with thiol metabolism
(2010) In Free Radical Biology & Medicine 48(2). p.216-222- Abstract
- We have previously shown that the use of a combination of antioxidants delayed the degeneration process in rd1 mouse retina. In an effort to understand the mechanism of action of these substances (zeaxanthin, lutein, alpha-lipoic acid, glutathione, and Lycium barbarum extract) the changes in the levels of several proteins and oxidative stress markers in the rd1 retina have been studied. The treatment increased glutathione peroxidase activity and glutathione levels and decreased cystine concentrations in rd1 retinas. Considering all the results obtained from treated and untreated animals, a high correlation was present between glutathione concentration and glutathione peroxidase activity, and there was a negative correlation between... (More)
- We have previously shown that the use of a combination of antioxidants delayed the degeneration process in rd1 mouse retina. In an effort to understand the mechanism of action of these substances (zeaxanthin, lutein, alpha-lipoic acid, glutathione, and Lycium barbarum extract) the changes in the levels of several proteins and oxidative stress markers in the rd1 retina have been studied. The treatment increased glutathione peroxidase activity and glutathione levels and decreased cystine concentrations in rd1 retinas. Considering all the results obtained from treated and untreated animals, a high correlation was present between glutathione concentration and glutathione peroxidase activity, and there was a negative correlation between glutathione retinal concentration and number of TUNEL-positive cells. No difference was observed between the numbers of nNOS- and NADPH-diaphorase-positive cells in treated and untreated rd1 mice. Thiol contents and thiol-dependent peroxide metabolism seem to be directly related to the survival of photoreceptors in rd1 mouse retina. (C) 2009 Elsevier Inc. All rights reserved. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1547320
- author
- Miranda, Maria ; Arnal, Emma ; Ahuja, Satpal LU ; Alvarez-Nolting, Raquel ; Lopez-Pedrajas, Rosa ; Ekström, Per LU ; Bosch-Morell, Francisco ; van Veen, Theo LU and Romero, Francisco J.
- organization
- publishing date
- 2010
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- radicals, Free, Nitric oxide, Glutathione, Antioxidants, Retina, Photoreceptors
- in
- Free Radical Biology & Medicine
- volume
- 48
- issue
- 2
- pages
- 216 - 222
- publisher
- Elsevier
- external identifiers
-
- wos:000273883800005
- scopus:72649084334
- ISSN
- 0891-5849
- DOI
- 10.1016/j.freeradbiomed.2009.10.042
- language
- English
- LU publication?
- yes
- id
- 946d1648-d033-496b-af09-53f1c5742132 (old id 1547320)
- date added to LUP
- 2016-04-01 10:13:20
- date last changed
- 2022-01-25 20:59:38
@article{946d1648-d033-496b-af09-53f1c5742132, abstract = {{We have previously shown that the use of a combination of antioxidants delayed the degeneration process in rd1 mouse retina. In an effort to understand the mechanism of action of these substances (zeaxanthin, lutein, alpha-lipoic acid, glutathione, and Lycium barbarum extract) the changes in the levels of several proteins and oxidative stress markers in the rd1 retina have been studied. The treatment increased glutathione peroxidase activity and glutathione levels and decreased cystine concentrations in rd1 retinas. Considering all the results obtained from treated and untreated animals, a high correlation was present between glutathione concentration and glutathione peroxidase activity, and there was a negative correlation between glutathione retinal concentration and number of TUNEL-positive cells. No difference was observed between the numbers of nNOS- and NADPH-diaphorase-positive cells in treated and untreated rd1 mice. Thiol contents and thiol-dependent peroxide metabolism seem to be directly related to the survival of photoreceptors in rd1 mouse retina. (C) 2009 Elsevier Inc. All rights reserved.}}, author = {{Miranda, Maria and Arnal, Emma and Ahuja, Satpal and Alvarez-Nolting, Raquel and Lopez-Pedrajas, Rosa and Ekström, Per and Bosch-Morell, Francisco and van Veen, Theo and Romero, Francisco J.}}, issn = {{0891-5849}}, keywords = {{radicals; Free; Nitric oxide; Glutathione; Antioxidants; Retina; Photoreceptors}}, language = {{eng}}, number = {{2}}, pages = {{216--222}}, publisher = {{Elsevier}}, series = {{Free Radical Biology & Medicine}}, title = {{Antioxidants rescue photoreceptors in rd1 mice: Relationship with thiol metabolism}}, url = {{http://dx.doi.org/10.1016/j.freeradbiomed.2009.10.042}}, doi = {{10.1016/j.freeradbiomed.2009.10.042}}, volume = {{48}}, year = {{2010}}, }