The interaction between DNA and cationic lipid films at the air-water interface
(2005) In Journal of Colloid and Interface Science 286(1). p.166-175- Abstract
- The interaction between DNA and positively charged dioctadecyldimethylammonium bromide (DODAB) and DODAB/disteroylphosphatidylcholine (DSPC) monolayers at the air-aqueous interface was studied by a combination of the surface film balance and Brewster angle microscopy. In presence of DNA, the &UPi;-A isotherm of the cationic monolayer shifts to larger mean molecular areas due to the electrostatic interaction with DNA while the typical liquid expanded-liquid condensed phase transition for DODAB monolayers disappear and the monolayer remains to be in the liquid expanded phase. Furthermore, the morphology of the film dramatically changes, where the large dendritic-like condensed aggregates observed for DODAB monolayers vanish. The charge... (More)
- The interaction between DNA and positively charged dioctadecyldimethylammonium bromide (DODAB) and DODAB/disteroylphosphatidylcholine (DSPC) monolayers at the air-aqueous interface was studied by a combination of the surface film balance and Brewster angle microscopy. In presence of DNA, the &UPi;-A isotherm of the cationic monolayer shifts to larger mean molecular areas due to the electrostatic interaction with DNA while the typical liquid expanded-liquid condensed phase transition for DODAB monolayers disappear and the monolayer remains to be in the liquid expanded phase. Furthermore, the morphology of the film dramatically changes, where the large dendritic-like condensed aggregates observed for DODAB monolayers vanish. The charge density of the monolayer was varied by using mixed monolayers with the zwitterionic DSPC and no large effect was observed on the interaction with DNA. By modeling the electrostatic interactions with the linearized Poisson-Boltzmann equation using the finite-element method and taking into account the assumption in the dielectric constants of the system, it was possible to corroborate the expansion of the cationic monolayer upon interaction with DNA as well as the fact that DNA does not seem to penetrate into the monolayer. © 2005 Elsevier Inc. All rights reserved. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/157248
- author
- Cárdenas, Marité LU ; Nylander, Tommy LU ; Jönsson, Bengt LU and Lindman, Björn LU
- organization
- publishing date
- 2005
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Journal of Colloid and Interface Science
- volume
- 286
- issue
- 1
- pages
- 166 - 175
- publisher
- Elsevier
- external identifiers
-
- wos:000228935700019
- scopus:17644380292
- ISSN
- 1095-7103
- DOI
- 10.1016/j.jcis.2005.01.008
- language
- English
- LU publication?
- yes
- additional info
- The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Theoretical Chemistry (S) (011001039), Physical Chemistry 1 (S) (011001006), Department of Chemistry (011001220)
- id
- ee448148-5cbc-4145-be06-23c95870d0fe (old id 157248)
- date added to LUP
- 2016-04-01 12:33:57
- date last changed
- 2022-04-21 17:11:33
@article{ee448148-5cbc-4145-be06-23c95870d0fe, abstract = {{The interaction between DNA and positively charged dioctadecyldimethylammonium bromide (DODAB) and DODAB/disteroylphosphatidylcholine (DSPC) monolayers at the air-aqueous interface was studied by a combination of the surface film balance and Brewster angle microscopy. In presence of DNA, the &UPi;-A isotherm of the cationic monolayer shifts to larger mean molecular areas due to the electrostatic interaction with DNA while the typical liquid expanded-liquid condensed phase transition for DODAB monolayers disappear and the monolayer remains to be in the liquid expanded phase. Furthermore, the morphology of the film dramatically changes, where the large dendritic-like condensed aggregates observed for DODAB monolayers vanish. The charge density of the monolayer was varied by using mixed monolayers with the zwitterionic DSPC and no large effect was observed on the interaction with DNA. By modeling the electrostatic interactions with the linearized Poisson-Boltzmann equation using the finite-element method and taking into account the assumption in the dielectric constants of the system, it was possible to corroborate the expansion of the cationic monolayer upon interaction with DNA as well as the fact that DNA does not seem to penetrate into the monolayer. © 2005 Elsevier Inc. All rights reserved.}}, author = {{Cárdenas, Marité and Nylander, Tommy and Jönsson, Bengt and Lindman, Björn}}, issn = {{1095-7103}}, language = {{eng}}, number = {{1}}, pages = {{166--175}}, publisher = {{Elsevier}}, series = {{Journal of Colloid and Interface Science}}, title = {{The interaction between DNA and cationic lipid films at the air-water interface}}, url = {{http://dx.doi.org/10.1016/j.jcis.2005.01.008}}, doi = {{10.1016/j.jcis.2005.01.008}}, volume = {{286}}, year = {{2005}}, }