Heparin-Binding Protein Release Is Strongly Induced by Leptospira Species and Is a Candidate for an Early Diagnostic Marker of Human Leptospirosis
Vieira, Mônica L. LU ; Persson, Sandra LU ; Lopes-Ferreira, Mônica ; Romero, Eliete C. ; Kirchgatter, Karin ; Nascimento, Ana Lucia T.O. and Herwald, Heiko LU
(2019)
In The Journal of infectious diseases
219(6).
p.996-1006
- Abstract
BACKGROUND: Leptospirosis, caused by spirochetes of the genus Leptospira, is one of the most widespread zoonoses worldwide. Efficient diagnostic methods for early diagnosis of leptospirosis are still lacking, and acute disease presents with nonspecific symptomatology and is often misdiagnosed. The leptospires pathogenic processes and virulence mechanisms remain virtually unknown. In severe infections, hemostatic impairment is frequently observed, and pathophysiological complications often develop when the host response is modulated by the pathogen. The neutrophil heparin-binding protein (HBP) is an inflammatory mediator and potent inducer of vascular leakage. RESULTS: In this study, we found that leptospires and their secreted products... (More)
BACKGROUND: Leptospirosis, caused by spirochetes of the genus Leptospira, is one of the most widespread zoonoses worldwide. Efficient diagnostic methods for early diagnosis of leptospirosis are still lacking, and acute disease presents with nonspecific symptomatology and is often misdiagnosed. The leptospires pathogenic processes and virulence mechanisms remain virtually unknown. In severe infections, hemostatic impairment is frequently observed, and pathophysiological complications often develop when the host response is modulated by the pathogen. The neutrophil heparin-binding protein (HBP) is an inflammatory mediator and potent inducer of vascular leakage. RESULTS: In this study, we found that leptospires and their secreted products induce the release of HBP from stimulated neutrophils through a controlled degranulation mechanism. We acknowledged 2 leptospiral proteins as able to induce HBP degranulation. These findings have clinical implications, as high levels of HBP were detected in serum from patients with leptospirosis, especially at the early phase of the disease. CONCLUSION: In conclusion, we describe a new mechanism by which the leptospirosis pathophysiological complications may arise, such as vascular leakage and edema formation. We also propose HBP as a new early screening biomarker for human leptospirosis.
(Less)
- author
- Vieira, Mônica L.
LU
; Persson, Sandra
LU
; Lopes-Ferreira, Mônica
; Romero, Eliete C.
; Kirchgatter, Karin
; Nascimento, Ana Lucia T.O.
and Herwald, Heiko
LU
- organization
- publishing date
- 2019
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Leptospira, host-pathogen-interactions, infection, inflammation, neutrophils
- in
- The Journal of infectious diseases
- volume
- 219
- issue
- 6
- pages
- 11 pages
- publisher
- Oxford University Press
- external identifiers
-
- pmid:30299510
- scopus:85062001599
- ISSN
- 1537-6613
- DOI
- 10.1093/infdis/jiy589
- language
- English
- LU publication?
- yes
- id
- 1612b699-d0d7-4a04-ac6d-cfb6ddfb8129
- date added to LUP
- 2019-03-06 10:42:36
- date last changed
- 2024-03-02 21:24:59
@article{1612b699-d0d7-4a04-ac6d-cfb6ddfb8129,
abstract = {{<p>BACKGROUND: Leptospirosis, caused by spirochetes of the genus Leptospira, is one of the most widespread zoonoses worldwide. Efficient diagnostic methods for early diagnosis of leptospirosis are still lacking, and acute disease presents with nonspecific symptomatology and is often misdiagnosed. The leptospires pathogenic processes and virulence mechanisms remain virtually unknown. In severe infections, hemostatic impairment is frequently observed, and pathophysiological complications often develop when the host response is modulated by the pathogen. The neutrophil heparin-binding protein (HBP) is an inflammatory mediator and potent inducer of vascular leakage. RESULTS: In this study, we found that leptospires and their secreted products induce the release of HBP from stimulated neutrophils through a controlled degranulation mechanism. We acknowledged 2 leptospiral proteins as able to induce HBP degranulation. These findings have clinical implications, as high levels of HBP were detected in serum from patients with leptospirosis, especially at the early phase of the disease. CONCLUSION: In conclusion, we describe a new mechanism by which the leptospirosis pathophysiological complications may arise, such as vascular leakage and edema formation. We also propose HBP as a new early screening biomarker for human leptospirosis.</p>}},
author = {{Vieira, Mônica L. and Persson, Sandra and Lopes-Ferreira, Mônica and Romero, Eliete C. and Kirchgatter, Karin and Nascimento, Ana Lucia T.O. and Herwald, Heiko}},
issn = {{1537-6613}},
keywords = {{Leptospira; host-pathogen-interactions; infection; inflammation; neutrophils}},
language = {{eng}},
number = {{6}},
pages = {{996--1006}},
publisher = {{Oxford University Press}},
series = {{The Journal of infectious diseases}},
title = {{Heparin-Binding Protein Release Is Strongly Induced by Leptospira Species and Is a Candidate for an Early Diagnostic Marker of Human Leptospirosis}},
url = {{http://dx.doi.org/10.1093/infdis/jiy589}},
doi = {{10.1093/infdis/jiy589}},
volume = {{219}},
year = {{2019}},
}