Linear dsDNA Partitions Spontaneously into the Inverse Hexagonal Lyotropic Liquid Crystalline Phases of Phospholipids.
(2010) In Journal of the American Chemical Society 132(28). p.9728-9732- Abstract
- Recently, we reported that DNA associated with inverse hexagonal (H(II)) lyotropic liquid crystal phases of the lipid 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine (DOPE) was actively transcribed by T7 RNA polymerase. (1) Our findings suggested that key components of the transcription process, probably the T7 RNA polymerase and the DNA, remained associated with the monolithic H(II) phase throughout transcription. Here, we investigate the partitioning of DNA between an H(II) lyotropic liquid crystal phase and an isotropic supernatant phase in order to develop insights into the localization of DNA in liquid crystalline environments. Our results show that linear double stranded DNA (dsDNA) molecules partition spontaneously into monolithic... (More)
- Recently, we reported that DNA associated with inverse hexagonal (H(II)) lyotropic liquid crystal phases of the lipid 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine (DOPE) was actively transcribed by T7 RNA polymerase. (1) Our findings suggested that key components of the transcription process, probably the T7 RNA polymerase and the DNA, remained associated with the monolithic H(II) phase throughout transcription. Here, we investigate the partitioning of DNA between an H(II) lyotropic liquid crystal phase and an isotropic supernatant phase in order to develop insights into the localization of DNA in liquid crystalline environments. Our results show that linear double stranded DNA (dsDNA) molecules partition spontaneously into monolithic preformed H(II) liquid crystal phases of DOPE. We propose that this process is driven by the increase in entropy due to the release of counterions from the DNA when it inserts into the aqueous pores of the H(II) phase. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1625734
- author
- Black, Camilla F ; Wilson, Richard J ; Nylander, Tommy LU ; Dymond, Marcus K and Attard, George S
- organization
- publishing date
- 2010
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Journal of the American Chemical Society
- volume
- 132
- issue
- 28
- pages
- 9728 - 9732
- publisher
- The American Chemical Society (ACS)
- external identifiers
-
- wos:000280086800049
- pmid:20575567
- scopus:77954643179
- pmid:20575567
- ISSN
- 1520-5126
- DOI
- 10.1021/ja101550c
- language
- English
- LU publication?
- yes
- id
- 9ff50112-6802-4dc6-8eb1-a2f2fa29428a (old id 1625734)
- date added to LUP
- 2016-04-01 14:09:56
- date last changed
- 2022-01-27 23:06:49
@article{9ff50112-6802-4dc6-8eb1-a2f2fa29428a, abstract = {{Recently, we reported that DNA associated with inverse hexagonal (H(II)) lyotropic liquid crystal phases of the lipid 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine (DOPE) was actively transcribed by T7 RNA polymerase. (1) Our findings suggested that key components of the transcription process, probably the T7 RNA polymerase and the DNA, remained associated with the monolithic H(II) phase throughout transcription. Here, we investigate the partitioning of DNA between an H(II) lyotropic liquid crystal phase and an isotropic supernatant phase in order to develop insights into the localization of DNA in liquid crystalline environments. Our results show that linear double stranded DNA (dsDNA) molecules partition spontaneously into monolithic preformed H(II) liquid crystal phases of DOPE. We propose that this process is driven by the increase in entropy due to the release of counterions from the DNA when it inserts into the aqueous pores of the H(II) phase.}}, author = {{Black, Camilla F and Wilson, Richard J and Nylander, Tommy and Dymond, Marcus K and Attard, George S}}, issn = {{1520-5126}}, language = {{eng}}, number = {{28}}, pages = {{9728--9732}}, publisher = {{The American Chemical Society (ACS)}}, series = {{Journal of the American Chemical Society}}, title = {{Linear dsDNA Partitions Spontaneously into the Inverse Hexagonal Lyotropic Liquid Crystalline Phases of Phospholipids.}}, url = {{http://dx.doi.org/10.1021/ja101550c}}, doi = {{10.1021/ja101550c}}, volume = {{132}}, year = {{2010}}, }