Demonstration of mitochondrial oestrogen receptor beta and oestrogen-induced attenuation of cytochrome c oxidase subunit I expression in human periodontal ligament cells.
Jönsson, Daniel LU ; Öhman, Jenny LU ; Odenlund, Malin LU ; Bratthall, Gunilla ; Broman, Jonas LU ; Ekblad, Eva LU ; Lydrup, Marie-Louise and Nilsson, Bengt-Olof LU
(2007)
In Archives of Oral Biology
52(7).
p.669-676
- Abstract
- OBJECTIVE: Periodontal ligament (PDL) cells express oestrogen receptor beta (ERbeta) protein, but cellular functions regulated by ERbeta in these cells have not been identified. In this study we determine if ERbeta is localised to mitochondria and if oestrogen regulates mitochondrial function in human PDL cells obtained from teeth extracted for orthodontic reasons. DESIGN: Subcellular distribution of ERbeta was determined by confocal microscopy of cells co-stained with ERbeta antibody and the mitochondrion-selective probe MitoTracker and by immunogold electron microscopy. Expression of the mitochondrial enzyme cytochrome c oxidase subunit I, involved in oxidative phosphorylation, was determined by Western blotting in cells treated with or... (More)
- OBJECTIVE: Periodontal ligament (PDL) cells express oestrogen receptor beta (ERbeta) protein, but cellular functions regulated by ERbeta in these cells have not been identified. In this study we determine if ERbeta is localised to mitochondria and if oestrogen regulates mitochondrial function in human PDL cells obtained from teeth extracted for orthodontic reasons. DESIGN: Subcellular distribution of ERbeta was determined by confocal microscopy of cells co-stained with ERbeta antibody and the mitochondrion-selective probe MitoTracker and by immunogold electron microscopy. Expression of the mitochondrial enzyme cytochrome c oxidase subunit I, involved in oxidative phosphorylation, was determined by Western blotting in cells treated with or without physiological concentrations of the endogenous oestrogen 17beta-oestradiol. RESULTS: ERbeta immunoreactivity was observed both in the nuclei and the cytoplasm. MitoTracker-labelling was observed in the cytoplasm, especially in the perinuclear region, but not in the nuclei. Co-localisation of ERbeta and MitoTracker was observed in cells derived from both male and female subjects. Mitochondrial localisation of ERbeta was confirmed by immunogold electron microscopy. Cells treated with or without 17beta-oestradiol (100 nM) displayed an identical pattern of staining for mitochondria. Treatment with 100 nM 17beta-oestradiol attenuated cytochrome c oxidase subunit I expression by about 30%, while combined treatment with 17beta-oestradiol and the ER blocker ICI 182780 (10 microM) had no effect. CONCLUSION: This study demonstrates mitochondrial localisation of ERbeta and oestrogen-induced decrease in the expression of cytochrome c oxidase subunit I in human PDL cells, suggesting that oestrogen probably via ERbeta influences mitochondrial function and PDL cell energy (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/164901
- author
- Jönsson, Daniel
LU
; Öhman, Jenny
LU
; Odenlund, Malin
LU
; Bratthall, Gunilla
; Broman, Jonas
LU
; Ekblad, Eva
LU
; Lydrup, Marie-Louise
and Nilsson, Bengt-Olof
LU
- organization
- publishing date
- 2007
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Oxidative phosphorylation, Mitochondria, Oestrogen, Immunocytochemistry, Periodontal ligament cells
- in
- Archives of Oral Biology
- volume
- 52
- issue
- 7
- pages
- 669 - 676
- publisher
- Elsevier
- external identifiers
-
- wos:000247120700010
- scopus:34247647421
- pmid:17223066
- ISSN
- 1879-1506
- DOI
- 10.1016/j.archoralbio.2006.12.009
- language
- English
- LU publication?
- yes
- additional info
- The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Vascular and Airway Research (LUR000005), Neurophysiology (013212004), Vascular Physiology (013212034), Neurogastroenterology (013212009)
- id
- 3dd7cae9-8b7d-4c16-9917-266532ec4fa1 (old id 164901)
- alternative location
- http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=17223066&dopt=Abstract
- date added to LUP
- 2016-04-01 11:39:17
- date last changed
- 2022-01-26 08:16:46
@article{3dd7cae9-8b7d-4c16-9917-266532ec4fa1,
abstract = {{OBJECTIVE: Periodontal ligament (PDL) cells express oestrogen receptor beta (ERbeta) protein, but cellular functions regulated by ERbeta in these cells have not been identified. In this study we determine if ERbeta is localised to mitochondria and if oestrogen regulates mitochondrial function in human PDL cells obtained from teeth extracted for orthodontic reasons. DESIGN: Subcellular distribution of ERbeta was determined by confocal microscopy of cells co-stained with ERbeta antibody and the mitochondrion-selective probe MitoTracker and by immunogold electron microscopy. Expression of the mitochondrial enzyme cytochrome c oxidase subunit I, involved in oxidative phosphorylation, was determined by Western blotting in cells treated with or without physiological concentrations of the endogenous oestrogen 17beta-oestradiol. RESULTS: ERbeta immunoreactivity was observed both in the nuclei and the cytoplasm. MitoTracker-labelling was observed in the cytoplasm, especially in the perinuclear region, but not in the nuclei. Co-localisation of ERbeta and MitoTracker was observed in cells derived from both male and female subjects. Mitochondrial localisation of ERbeta was confirmed by immunogold electron microscopy. Cells treated with or without 17beta-oestradiol (100 nM) displayed an identical pattern of staining for mitochondria. Treatment with 100 nM 17beta-oestradiol attenuated cytochrome c oxidase subunit I expression by about 30%, while combined treatment with 17beta-oestradiol and the ER blocker ICI 182780 (10 microM) had no effect. CONCLUSION: This study demonstrates mitochondrial localisation of ERbeta and oestrogen-induced decrease in the expression of cytochrome c oxidase subunit I in human PDL cells, suggesting that oestrogen probably via ERbeta influences mitochondrial function and PDL cell energy}},
author = {{Jönsson, Daniel and Öhman, Jenny and Odenlund, Malin and Bratthall, Gunilla and Broman, Jonas and Ekblad, Eva and Lydrup, Marie-Louise and Nilsson, Bengt-Olof}},
issn = {{1879-1506}},
keywords = {{Oxidative phosphorylation; Mitochondria; Oestrogen; Immunocytochemistry; Periodontal ligament cells}},
language = {{eng}},
number = {{7}},
pages = {{669--676}},
publisher = {{Elsevier}},
series = {{Archives of Oral Biology}},
title = {{Demonstration of mitochondrial oestrogen receptor beta and oestrogen-induced attenuation of cytochrome c oxidase subunit I expression in human periodontal ligament cells.}},
url = {{http://dx.doi.org/10.1016/j.archoralbio.2006.12.009}},
doi = {{10.1016/j.archoralbio.2006.12.009}},
volume = {{52}},
year = {{2007}},
}