Mesenchymal stromal cells from primary osteosarcoma are non-malignant and strikingly similar to their bone marrow counterparts.
(2011) In International Journal of Cancer 129. p.319-330- Abstract
- Mesenchymal stromal cells (MSC) are multipotent cells that can be isolated from a number of human tissues. In cancer, MSC have been implicated with tumor growth, invasion, metastasis, drug resistance and were even suggested as possible tumor-initiating cells in osteosarcoma (OS). However, MSC from OS and their possible tumor origin have not yet been thoroughly investigated. Therefore, primary OS mesenchymal progenitors and OS-derived MSC were studied. OS samples contained very high frequencies of mesenchymal progenitor cells as measured by the CFU-F assay (median: 1,117 colonies per 10(5) cells, range: 133 - 3,000, n=6). This is considerably higher compared to other human tissues such as normal bone marrow (1.3 ± 0.2 colonies per 10(5)... (More)
- Mesenchymal stromal cells (MSC) are multipotent cells that can be isolated from a number of human tissues. In cancer, MSC have been implicated with tumor growth, invasion, metastasis, drug resistance and were even suggested as possible tumor-initiating cells in osteosarcoma (OS). However, MSC from OS and their possible tumor origin have not yet been thoroughly investigated. Therefore, primary OS mesenchymal progenitors and OS-derived MSC were studied. OS samples contained very high frequencies of mesenchymal progenitor cells as measured by the CFU-F assay (median: 1,117 colonies per 10(5) cells, range: 133 - 3,000, n=6). This is considerably higher compared to other human tissues such as normal bone marrow (1.3 ± 0.2 colonies per 10(5) cells, n=8). OS-derived MSC (OS-MSC) showed normal MSC morphology and expressed the typical MSC surface marker profile (CD105/CD73/CD90/CD44/HLA-classI/CD166 positive, CD45/CD34/CD14/CD19/HLA-DR/CD31 negative). Furthermore, all OS-MSC samples could be differentiated into the osteogenic lineage, and all but one sample into adipocytes and chondrocytes. Genetic analysis of OS-MSC as well as OS-derived spheres showed no tumor-related chromosomal aberrations. OS-MSC expression of markers related to tumor-associated fibroblasts (fibroblast surface protein, alpha-smooth muscle actin, vimentin) was comparable to bone marrow MSC and OS-MSC growth was considerably affected by tyrosine kinase inhibitors. Taken together, our results demonstrate that normal, non-malignant mesenchymal stroma cells are isolated from OS when MSC culture techniques are applied. OS-MSC represent a major constituent of the tumor microenvironment, and they share many properties with bone marrow-derived MSC. © 2010 UICC. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1687657
- author
- Brune, Jan Claas LU ; Tormin, Ariane LU ; Johansson, Maria C LU ; Rissler, Pehr LU ; Brosjö, Otte ; Löfvenberg, Richard ; Vult von Steyern, Fredrik LU ; Mertens, Fredrik LU ; Rydholm, Anders LU and Scheding, Stefan LU
- organization
- publishing date
- 2011
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- mesenchymal stromal cells, osteosarcoma, osteogenic sarcoma, cancer associated fibroblasts, tumor stroma
- in
- International Journal of Cancer
- volume
- 129
- pages
- 319 - 330
- publisher
- John Wiley & Sons Inc.
- external identifiers
-
- wos:000291603900006
- pmid:20878957
- scopus:79961123373
- ISSN
- 0020-7136
- DOI
- 10.1002/ijc.25697
- language
- English
- LU publication?
- yes
- additional info
- The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Division of Clinical Genetics (013022003), Department of Orthopaedics (Lund) (013028000), Oncology, MV (013035000), Stem Cell Center (013041110), Pathology, (Lund) (013030000)
- id
- e543d695-004d-4aa6-8edd-71c9479cbf1f (old id 1687657)
- alternative location
- http://www.ncbi.nlm.nih.gov/pubmed/20878957?dopt=Abstract
- date added to LUP
- 2016-04-01 11:04:09
- date last changed
- 2022-08-27 23:00:31
@article{e543d695-004d-4aa6-8edd-71c9479cbf1f, abstract = {{Mesenchymal stromal cells (MSC) are multipotent cells that can be isolated from a number of human tissues. In cancer, MSC have been implicated with tumor growth, invasion, metastasis, drug resistance and were even suggested as possible tumor-initiating cells in osteosarcoma (OS). However, MSC from OS and their possible tumor origin have not yet been thoroughly investigated. Therefore, primary OS mesenchymal progenitors and OS-derived MSC were studied. OS samples contained very high frequencies of mesenchymal progenitor cells as measured by the CFU-F assay (median: 1,117 colonies per 10(5) cells, range: 133 - 3,000, n=6). This is considerably higher compared to other human tissues such as normal bone marrow (1.3 ± 0.2 colonies per 10(5) cells, n=8). OS-derived MSC (OS-MSC) showed normal MSC morphology and expressed the typical MSC surface marker profile (CD105/CD73/CD90/CD44/HLA-classI/CD166 positive, CD45/CD34/CD14/CD19/HLA-DR/CD31 negative). Furthermore, all OS-MSC samples could be differentiated into the osteogenic lineage, and all but one sample into adipocytes and chondrocytes. Genetic analysis of OS-MSC as well as OS-derived spheres showed no tumor-related chromosomal aberrations. OS-MSC expression of markers related to tumor-associated fibroblasts (fibroblast surface protein, alpha-smooth muscle actin, vimentin) was comparable to bone marrow MSC and OS-MSC growth was considerably affected by tyrosine kinase inhibitors. Taken together, our results demonstrate that normal, non-malignant mesenchymal stroma cells are isolated from OS when MSC culture techniques are applied. OS-MSC represent a major constituent of the tumor microenvironment, and they share many properties with bone marrow-derived MSC. © 2010 UICC.}}, author = {{Brune, Jan Claas and Tormin, Ariane and Johansson, Maria C and Rissler, Pehr and Brosjö, Otte and Löfvenberg, Richard and Vult von Steyern, Fredrik and Mertens, Fredrik and Rydholm, Anders and Scheding, Stefan}}, issn = {{0020-7136}}, keywords = {{mesenchymal stromal cells; osteosarcoma; osteogenic sarcoma; cancer associated fibroblasts; tumor stroma}}, language = {{eng}}, pages = {{319--330}}, publisher = {{John Wiley & Sons Inc.}}, series = {{International Journal of Cancer}}, title = {{Mesenchymal stromal cells from primary osteosarcoma are non-malignant and strikingly similar to their bone marrow counterparts.}}, url = {{http://dx.doi.org/10.1002/ijc.25697}}, doi = {{10.1002/ijc.25697}}, volume = {{129}}, year = {{2011}}, }