Induction of precocious intestinal maturation in T-cell deficient athymic neonatal rats
(2017) In World Journal of Gastroenterology 23(42). p.7531-7540- Abstract
Aim: To investigate whether gut maturation could be induced precociously in an athymic T-cell deficient neonatal rat model. Methods: Fourteen day-old athymic (nude) rats (NIH-Foxn 1rnu) were gavaged with either phytohaemagglutinin - a lectin from red kidney beans (PHA); trypsin - a protease (Prot); or water - vehicle (control) as a single dose on one day or once a day for 3-day. The nude rats were either nurtured by their mothers or cross-fostered by conventional foster dams of the Sprague-Dawley strain from days 3-5 after birth. At 17 d of age, 72 h after administration of the first treatment, intestinal macromolecular permeability was tested in vivo, prior to euthanasia, after which blood and gut organs were sampled.... (More)
Aim: To investigate whether gut maturation could be induced precociously in an athymic T-cell deficient neonatal rat model. Methods: Fourteen day-old athymic (nude) rats (NIH-Foxn 1rnu) were gavaged with either phytohaemagglutinin - a lectin from red kidney beans (PHA); trypsin - a protease (Prot); or water - vehicle (control) as a single dose on one day or once a day for 3-day. The nude rats were either nurtured by their mothers or cross-fostered by conventional foster dams of the Sprague-Dawley strain from days 3-5 after birth. At 17 d of age, 72 h after administration of the first treatment, intestinal macromolecular permeability was tested in vivo, prior to euthanasia, after which blood and gut organs were sampled. Results: Provocation with both, PHA and protease, resulted in increased gut growth and maturation in nude rat pups independent of nursing. Foetal-type enterocytes were replaced by non-vacuolated adult-type enterocytes in the distal small intestine epithelium. Decreased intestinal macromolecular permeability (gut closure) was observed, with reduced permeability markers (BIgG and BSA, P < 0.001) in circulation. Increased pancreatic function, with an increased trypsin to protein ratio in pancreas homogenates, was observed independent of nursing in the nude pups. Immunostaining showed the presence of a few CD3+-cells in the intestinal mucosa of the nude pups. The number of CD3+-cells remained unaltered by provocation and no differences were observed between the nursing sets. Growth and vitality of the nude pups were dependent on nurturing, since cross-fostering by conventional dams increased their macromolecular absorptive capacity (BSA, P < 0.05), as well as their passive immunity (RIgG, P < 0.05). Conclusion: Precocious gut maturation can be induced by enteral provocation in athymic rat pups, similarly to in euthymic pups, thus showing an independence from thymusderived T-cells.
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- author
- Sureda, Ester Arévalo LU ; Gidlund, Catherine ; Weström, Björn LU and Prykhodko, Olena LU
- organization
- publishing date
- 2017-11-14
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Gut, Immunoglobulin G, Intestinal permeability, Pancreas, Passive immunity
- in
- World Journal of Gastroenterology
- volume
- 23
- issue
- 42
- pages
- 10 pages
- publisher
- WJG Press
- external identifiers
-
- pmid:29204053
- wos:000414872500004
- scopus:85034108416
- ISSN
- 1007-9327
- DOI
- 10.3748/wjg.v23.i42.7531
- language
- English
- LU publication?
- yes
- id
- 16c5719d-2e6e-4a26-a0c6-b1494050151e
- date added to LUP
- 2017-12-19 13:13:07
- date last changed
- 2024-04-14 21:33:20
@article{16c5719d-2e6e-4a26-a0c6-b1494050151e,
abstract = {{<p>Aim: To investigate whether gut maturation could be induced precociously in an athymic T-cell deficient neonatal rat model. Methods: Fourteen day-old athymic (nude) rats (NIH-Foxn 1<sup>rnu</sup>) were gavaged with either phytohaemagglutinin - a lectin from red kidney beans (PHA); trypsin - a protease (Prot); or water - vehicle (control) as a single dose on one day or once a day for 3-day. The nude rats were either nurtured by their mothers or cross-fostered by conventional foster dams of the Sprague-Dawley strain from days 3-5 after birth. At 17 d of age, 72 h after administration of the first treatment, intestinal macromolecular permeability was tested in vivo, prior to euthanasia, after which blood and gut organs were sampled. Results: Provocation with both, PHA and protease, resulted in increased gut growth and maturation in nude rat pups independent of nursing. Foetal-type enterocytes were replaced by non-vacuolated adult-type enterocytes in the distal small intestine epithelium. Decreased intestinal macromolecular permeability (gut closure) was observed, with reduced permeability markers (BIgG and BSA, P < 0.001) in circulation. Increased pancreatic function, with an increased trypsin to protein ratio in pancreas homogenates, was observed independent of nursing in the nude pups. Immunostaining showed the presence of a few CD3<sup>+</sup>-cells in the intestinal mucosa of the nude pups. The number of CD3<sup>+</sup>-cells remained unaltered by provocation and no differences were observed between the nursing sets. Growth and vitality of the nude pups were dependent on nurturing, since cross-fostering by conventional dams increased their macromolecular absorptive capacity (BSA, P < 0.05), as well as their passive immunity (RIgG, P < 0.05). Conclusion: Precocious gut maturation can be induced by enteral provocation in athymic rat pups, similarly to in euthymic pups, thus showing an independence from thymusderived T-cells.</p>}},
author = {{Sureda, Ester Arévalo and Gidlund, Catherine and Weström, Björn and Prykhodko, Olena}},
issn = {{1007-9327}},
keywords = {{Gut; Immunoglobulin G; Intestinal permeability; Pancreas; Passive immunity}},
language = {{eng}},
month = {{11}},
number = {{42}},
pages = {{7531--7540}},
publisher = {{WJG Press}},
series = {{World Journal of Gastroenterology}},
title = {{Induction of precocious intestinal maturation in T-cell deficient athymic neonatal rats}},
url = {{http://dx.doi.org/10.3748/wjg.v23.i42.7531}},
doi = {{10.3748/wjg.v23.i42.7531}},
volume = {{23}},
year = {{2017}},
}