Helicobacter species and common gut bacterial DNA in gallbladder with cholecystitis.
(2010) In World Journal of Gastroenterology 16(38). p.4817-4822- Abstract
- AIM: To analyze the association between Helicobacter spp. and some common gut bacteria in patients with cholecystitis. METHODS: A nested-polymerase chain reaction (PCR), specific to 16S rRNA of Helicobacter spp. was performed on paraffin-embedded gallbladder samples of 100 cholecystitis and 102 control cases. The samples were also analyzed for some common gut bacteria by PCR. Positive samples were sequenced for species identification. RESULTS: Helicobacter DNA was found in seven out of 100 cases of acute and chronic cholecystitis. Sequence analysis displayed Helicobacter pullorum (H. pullorum) in six cases and Helicobacter pylori in one; H. pullorum was only found in cases with metaplasia. Control samples were negative for Helicobacter... (More)
- AIM: To analyze the association between Helicobacter spp. and some common gut bacteria in patients with cholecystitis. METHODS: A nested-polymerase chain reaction (PCR), specific to 16S rRNA of Helicobacter spp. was performed on paraffin-embedded gallbladder samples of 100 cholecystitis and 102 control cases. The samples were also analyzed for some common gut bacteria by PCR. Positive samples were sequenced for species identification. RESULTS: Helicobacter DNA was found in seven out of 100 cases of acute and chronic cholecystitis. Sequence analysis displayed Helicobacter pullorum (H. pullorum) in six cases and Helicobacter pylori in one; H. pullorum was only found in cases with metaplasia. Control samples were negative for Helicobacter spp. and some common gut bacteria. There was a significant difference (P = 0.007) between cholecystitis and control samples for Helicobacter DNA. CONCLUSION: A possible relationship was detected between Helicobacter DNA and cholecystitis. Further serological and immunohistochemical studies are needed to support these data. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1711284
- author
- Karagin, Peren H ; Stenram, Unne LU ; Wadström, Torkel LU and Ljungh, Åsa LU
- organization
- publishing date
- 2010
- type
- Contribution to journal
- publication status
- published
- subject
- in
- World Journal of Gastroenterology
- volume
- 16
- issue
- 38
- pages
- 4817 - 4822
- publisher
- WJG Press
- external identifiers
-
- wos:000283185800008
- pmid:20939110
- scopus:78049487203
- ISSN
- 1007-9327
- DOI
- 10.3748/wjg.v16.i38.4817
- language
- English
- LU publication?
- yes
- additional info
- The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Pathology, (Lund) (013030000), Division of Medical Microbiology (013250400)
- id
- c9af5984-3bf8-4c82-b4d4-0dd2006cd51c (old id 1711284)
- alternative location
- http://www.ncbi.nlm.nih.gov/pubmed/20939110?dopt=Abstract
- date added to LUP
- 2016-04-04 07:13:34
- date last changed
- 2022-01-29 01:55:02
@article{c9af5984-3bf8-4c82-b4d4-0dd2006cd51c, abstract = {{AIM: To analyze the association between Helicobacter spp. and some common gut bacteria in patients with cholecystitis. METHODS: A nested-polymerase chain reaction (PCR), specific to 16S rRNA of Helicobacter spp. was performed on paraffin-embedded gallbladder samples of 100 cholecystitis and 102 control cases. The samples were also analyzed for some common gut bacteria by PCR. Positive samples were sequenced for species identification. RESULTS: Helicobacter DNA was found in seven out of 100 cases of acute and chronic cholecystitis. Sequence analysis displayed Helicobacter pullorum (H. pullorum) in six cases and Helicobacter pylori in one; H. pullorum was only found in cases with metaplasia. Control samples were negative for Helicobacter spp. and some common gut bacteria. There was a significant difference (P = 0.007) between cholecystitis and control samples for Helicobacter DNA. CONCLUSION: A possible relationship was detected between Helicobacter DNA and cholecystitis. Further serological and immunohistochemical studies are needed to support these data.}}, author = {{Karagin, Peren H and Stenram, Unne and Wadström, Torkel and Ljungh, Åsa}}, issn = {{1007-9327}}, language = {{eng}}, number = {{38}}, pages = {{4817--4822}}, publisher = {{WJG Press}}, series = {{World Journal of Gastroenterology}}, title = {{Helicobacter species and common gut bacterial DNA in gallbladder with cholecystitis.}}, url = {{http://dx.doi.org/10.3748/wjg.v16.i38.4817}}, doi = {{10.3748/wjg.v16.i38.4817}}, volume = {{16}}, year = {{2010}}, }