Differences in islet-enriched miRNAs in healthy and glucose intolerant human subjects.
(2011) In Biochemical and Biophysical Research Communications Dec. p.16-22- Abstract
- Many microRNAs (miRNAs) are known to be cell-type specific and are implicated in development of diseases. We investigated the global expression pattern of miRNAs in human pancreatic islets compared to liver and skeletal muscle, using bead-based technology and quantitative RT-PCR. In addition to the known islet-specific miR-375, we also found enrichment of miR-127-3p, miR-184, miR-195 and miR-493∗ in the pancreatic islets. The expression of miR-375, miR-127-3p, miR-184 and the liver-enriched miR-122 were positively correlated to insulin biosynthesis, while the expression of miR-127-3p and miR-184 were negatively correlated to glucose-stimulated insulin secretion (GSIS). These correlations were absent in islets of glucose intolerant donors... (More)
- Many microRNAs (miRNAs) are known to be cell-type specific and are implicated in development of diseases. We investigated the global expression pattern of miRNAs in human pancreatic islets compared to liver and skeletal muscle, using bead-based technology and quantitative RT-PCR. In addition to the known islet-specific miR-375, we also found enrichment of miR-127-3p, miR-184, miR-195 and miR-493∗ in the pancreatic islets. The expression of miR-375, miR-127-3p, miR-184 and the liver-enriched miR-122 were positively correlated to insulin biosynthesis, while the expression of miR-127-3p and miR-184 were negatively correlated to glucose-stimulated insulin secretion (GSIS). These correlations were absent in islets of glucose intolerant donors (HbA1c⩾6.1). We suggest the presence of an islet-specific miRNA network, which consists of at least miR-375, miR-127-3p and miR-184, potentially involved in insulin secretion. Our results provide new insight into miRNA-mediated regulation of insulin secretion in healthy and glucose intolerant subjects. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1731717
- author
- Bolmeson, Caroline LU ; Esguerra, Jonathan LU ; Salehi, S Albert LU ; Speidel, Dina LU ; Eliasson, Lena LU and Cilio, Corrado LU
- organization
- publishing date
- 2011
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Biochemical and Biophysical Research Communications
- volume
- Dec
- pages
- 16 - 22
- publisher
- Elsevier
- external identifiers
-
- wos:000286487700004
- pmid:21094635
- scopus:78650902669
- pmid:21094635
- ISSN
- 1090-2104
- DOI
- 10.1016/j.bbrc.2010.11.024
- language
- English
- LU publication?
- yes
- id
- 9ffc6bca-c591-4b85-a04b-a94f6b0eb346 (old id 1731717)
- alternative location
- http://www.ncbi.nlm.nih.gov/pubmed/21094635?dopt=Abstract
- date added to LUP
- 2016-04-04 09:08:17
- date last changed
- 2022-04-15 21:55:41
@article{9ffc6bca-c591-4b85-a04b-a94f6b0eb346, abstract = {{Many microRNAs (miRNAs) are known to be cell-type specific and are implicated in development of diseases. We investigated the global expression pattern of miRNAs in human pancreatic islets compared to liver and skeletal muscle, using bead-based technology and quantitative RT-PCR. In addition to the known islet-specific miR-375, we also found enrichment of miR-127-3p, miR-184, miR-195 and miR-493∗ in the pancreatic islets. The expression of miR-375, miR-127-3p, miR-184 and the liver-enriched miR-122 were positively correlated to insulin biosynthesis, while the expression of miR-127-3p and miR-184 were negatively correlated to glucose-stimulated insulin secretion (GSIS). These correlations were absent in islets of glucose intolerant donors (HbA1c⩾6.1). We suggest the presence of an islet-specific miRNA network, which consists of at least miR-375, miR-127-3p and miR-184, potentially involved in insulin secretion. Our results provide new insight into miRNA-mediated regulation of insulin secretion in healthy and glucose intolerant subjects.}}, author = {{Bolmeson, Caroline and Esguerra, Jonathan and Salehi, S Albert and Speidel, Dina and Eliasson, Lena and Cilio, Corrado}}, issn = {{1090-2104}}, language = {{eng}}, pages = {{16--22}}, publisher = {{Elsevier}}, series = {{Biochemical and Biophysical Research Communications}}, title = {{Differences in islet-enriched miRNAs in healthy and glucose intolerant human subjects.}}, url = {{http://dx.doi.org/10.1016/j.bbrc.2010.11.024}}, doi = {{10.1016/j.bbrc.2010.11.024}}, volume = {{Dec}}, year = {{2011}}, }