Size separations of starch of different botanical origin studied by asymmetrical-flow field-flow fractionation and multiangle light scattering.
(2011) In Analytical and Bioanalytical Chemistry 399. p.1455-1465- Abstract
- Asymmetrical-flow field-flow fractionation combined with multiangle light scattering and refractive index detection has been revealed to be a powerful tool for starch characterization. It is based on size separation according to the hydrodynamic diameter of the starch components. Starch from a wide range of different botanical sources were studied, including normal starch and high-amylose and high-amylopectin starch. The starch was dissolved by heat treatment at elevated pressure in a laboratory autoclave. This gave clear solutions with no granular residues. Amylose retrogradation was prevented by using freshly dissolved samples. Programmed cross flow starting at 1.0 mL min(-1) and decreasing exponentially with a half-life of 4 min was... (More)
- Asymmetrical-flow field-flow fractionation combined with multiangle light scattering and refractive index detection has been revealed to be a powerful tool for starch characterization. It is based on size separation according to the hydrodynamic diameter of the starch components. Starch from a wide range of different botanical sources were studied, including normal starch and high-amylose and high-amylopectin starch. The starch was dissolved by heat treatment at elevated pressure in a laboratory autoclave. This gave clear solutions with no granular residues. Amylose retrogradation was prevented by using freshly dissolved samples. Programmed cross flow starting at 1.0 mL min(-1) and decreasing exponentially with a half-life of 4 min was utilised. The starches showed two size populations representing mainly amylose and mainly amylopectin with an overlapping region where amylose and amylopectin were possibly co-eluted. Most of the first population had molar masses below 10(6) g mol(-1), and most of the second size population had molar masses above 10(7) g mol(-1). Large differences were found in the relative amounts of the two populations, the molar mass, and hydrodynamic diameters, depending on the plant source and its varieties. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1755843
- author
- Wahlund, Karl-Gustav LU ; Leeman, Mats LU and Santacruz, Stalin LU
- organization
- publishing date
- 2011
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Analytical and Bioanalytical Chemistry
- volume
- 399
- pages
- 1455 - 1465
- publisher
- Springer
- external identifiers
-
- wos:000286599200007
- pmid:21181137
- scopus:79451475321
- pmid:21181137
- ISSN
- 1618-2642
- DOI
- 10.1007/s00216-010-4438-5
- language
- English
- LU publication?
- yes
- additional info
- The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Organic chemistry (S/LTH) (011001240), Department of Chemistry (011001220)
- id
- 67beb767-47c2-48f0-898a-618f497a099e (old id 1755843)
- date added to LUP
- 2016-04-01 10:57:20
- date last changed
- 2022-02-17 22:51:55
@article{67beb767-47c2-48f0-898a-618f497a099e,
abstract = {{Asymmetrical-flow field-flow fractionation combined with multiangle light scattering and refractive index detection has been revealed to be a powerful tool for starch characterization. It is based on size separation according to the hydrodynamic diameter of the starch components. Starch from a wide range of different botanical sources were studied, including normal starch and high-amylose and high-amylopectin starch. The starch was dissolved by heat treatment at elevated pressure in a laboratory autoclave. This gave clear solutions with no granular residues. Amylose retrogradation was prevented by using freshly dissolved samples. Programmed cross flow starting at 1.0 mL min(-1) and decreasing exponentially with a half-life of 4 min was utilised. The starches showed two size populations representing mainly amylose and mainly amylopectin with an overlapping region where amylose and amylopectin were possibly co-eluted. Most of the first population had molar masses below 10(6) g mol(-1), and most of the second size population had molar masses above 10(7) g mol(-1). Large differences were found in the relative amounts of the two populations, the molar mass, and hydrodynamic diameters, depending on the plant source and its varieties.}},
author = {{Wahlund, Karl-Gustav and Leeman, Mats and Santacruz, Stalin}},
issn = {{1618-2642}},
language = {{eng}},
pages = {{1455--1465}},
publisher = {{Springer}},
series = {{Analytical and Bioanalytical Chemistry}},
title = {{Size separations of starch of different botanical origin studied by asymmetrical-flow field-flow fractionation and multiangle light scattering.}},
url = {{http://dx.doi.org/10.1007/s00216-010-4438-5}},
doi = {{10.1007/s00216-010-4438-5}},
volume = {{399}},
year = {{2011}},
}