Coomassie Staining as Loading Control in Western Blot Analysis
(2011) In Journal of Proteome Research 10(3). p.1416-1419- Abstract
- In Western blotting, immunodetection of housekeeping proteins is routinely performed to detect differences in electrophoresis loading. The present work describes a much faster and simpler protein staining method, which is compatible with ordinary blocking conditions. In addition, the method can be used after immunodetection with superior linearity compared to ordinary staining methods. After immunoblotting and staining, protein bands can be further identified using peptide mass fingerprinting.
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1869139
- author
- Welinder, Charlotte LU and Ekblad, Lars LU
- organization
- publishing date
- 2011
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Western blot, loading control, Coomassie, MALDI-TOF, PVDF, GAPDH, peptide mass fingerprinting
- in
- Journal of Proteome Research
- volume
- 10
- issue
- 3
- pages
- 1416 - 1419
- publisher
- The American Chemical Society (ACS)
- external identifiers
-
- wos:000287944000044
- scopus:79952400632
- pmid:21186791
- ISSN
- 1535-3893
- DOI
- 10.1021/pr1011476
- language
- English
- LU publication?
- yes
- id
- bc5d1c6b-57f5-48f7-b597-b6f48d922d87 (old id 1869139)
- date added to LUP
- 2016-04-01 11:13:45
- date last changed
- 2022-04-20 17:56:28
@article{bc5d1c6b-57f5-48f7-b597-b6f48d922d87, abstract = {{In Western blotting, immunodetection of housekeeping proteins is routinely performed to detect differences in electrophoresis loading. The present work describes a much faster and simpler protein staining method, which is compatible with ordinary blocking conditions. In addition, the method can be used after immunodetection with superior linearity compared to ordinary staining methods. After immunoblotting and staining, protein bands can be further identified using peptide mass fingerprinting.}}, author = {{Welinder, Charlotte and Ekblad, Lars}}, issn = {{1535-3893}}, keywords = {{Western blot; loading control; Coomassie; MALDI-TOF; PVDF; GAPDH; peptide mass fingerprinting}}, language = {{eng}}, number = {{3}}, pages = {{1416--1419}}, publisher = {{The American Chemical Society (ACS)}}, series = {{Journal of Proteome Research}}, title = {{Coomassie Staining as Loading Control in Western Blot Analysis}}, url = {{http://dx.doi.org/10.1021/pr1011476}}, doi = {{10.1021/pr1011476}}, volume = {{10}}, year = {{2011}}, }