Excitation-Emission Polarization Spectroscopy of Single Light Harvesting Complexes.
(2011) In The Journal of Physical Chemistry Part B 115(17). p.4963-4970- Abstract
- Excitation and emission polarization dependence of fluorescence intensity of single LH2 complexes from Rhodopseudomonas acidophila 10050 and Rhodobacter sphaeroides is reported. The results are presented as two-dimensional polarization plots and interpreted in terms of tilted light harvesting complexes indicating that sample preparation leads to partially oriented LH2 cylinders. An alternative explanation of the observation can be structural deformation. Fluorescence intensity of the complexes has four qualitatively distinct excitation-emission polarization dependencies. The differences in excitation polarization dependence are interpreted as due to the tilt of the complexes, whereas the emission polarization behavior is mainly determined... (More)
- Excitation and emission polarization dependence of fluorescence intensity of single LH2 complexes from Rhodopseudomonas acidophila 10050 and Rhodobacter sphaeroides is reported. The results are presented as two-dimensional polarization plots and interpreted in terms of tilted light harvesting complexes indicating that sample preparation leads to partially oriented LH2 cylinders. An alternative explanation of the observation can be structural deformation. Fluorescence intensity of the complexes has four qualitatively distinct excitation-emission polarization dependencies. The differences in excitation polarization dependence are interpreted as due to the tilt of the complexes, whereas the emission polarization behavior is mainly determined by spectral inhomogeneity of the emitting B850 ring. Some complexes show abrupt reversible variations of the total emission intensity together with changes of the polarization properties which cannot be described by the simplest model of tilted LH2s with spectral disorder. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1937230
- author
- Tubasum, Sumera LU ; Cogdell, Richard J ; Scheblykin, Ivan LU and Pullerits, Tönu LU
- organization
- publishing date
- 2011
- type
- Contribution to journal
- publication status
- published
- subject
- in
- The Journal of Physical Chemistry Part B
- volume
- 115
- issue
- 17
- pages
- 4963 - 4970
- publisher
- The American Chemical Society (ACS)
- external identifiers
-
- wos:000289824300008
- pmid:21486039
- scopus:79955488490
- pmid:21486039
- ISSN
- 1520-5207
- DOI
- 10.1021/jp107480x
- language
- English
- LU publication?
- yes
- additional info
- The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Chemical Physics (S) (011001060)
- id
- 716aae84-1320-4417-b1fe-2436cf1c9069 (old id 1937230)
- date added to LUP
- 2016-04-01 13:46:36
- date last changed
- 2022-04-14 03:05:20
@article{716aae84-1320-4417-b1fe-2436cf1c9069, abstract = {{Excitation and emission polarization dependence of fluorescence intensity of single LH2 complexes from Rhodopseudomonas acidophila 10050 and Rhodobacter sphaeroides is reported. The results are presented as two-dimensional polarization plots and interpreted in terms of tilted light harvesting complexes indicating that sample preparation leads to partially oriented LH2 cylinders. An alternative explanation of the observation can be structural deformation. Fluorescence intensity of the complexes has four qualitatively distinct excitation-emission polarization dependencies. The differences in excitation polarization dependence are interpreted as due to the tilt of the complexes, whereas the emission polarization behavior is mainly determined by spectral inhomogeneity of the emitting B850 ring. Some complexes show abrupt reversible variations of the total emission intensity together with changes of the polarization properties which cannot be described by the simplest model of tilted LH2s with spectral disorder.}}, author = {{Tubasum, Sumera and Cogdell, Richard J and Scheblykin, Ivan and Pullerits, Tönu}}, issn = {{1520-5207}}, language = {{eng}}, number = {{17}}, pages = {{4963--4970}}, publisher = {{The American Chemical Society (ACS)}}, series = {{The Journal of Physical Chemistry Part B}}, title = {{Excitation-Emission Polarization Spectroscopy of Single Light Harvesting Complexes.}}, url = {{http://dx.doi.org/10.1021/jp107480x}}, doi = {{10.1021/jp107480x}}, volume = {{115}}, year = {{2011}}, }