SO2-catalyzed steam pretreatment and fermentation of enzymatically hydrolyzed sugarcane bagasse
(2010) In Enzyme and Microbial Technology 46(2). p.64-73- Abstract
- Sugarcane bagasse is a lignocellulosic residue obtained from sugarcane milling, and a potentially interesting
raw material that can be used for fuel ethanol production. In the present study, bagasse was
steam pretreated at temperatures between 180 and 205 ◦C, with holding times of 5–10 min using SO2
as a catalyst to determine conditions that provide a good recovery of pentoses and a suitable material
for enzymatic hydrolysis. Pretreatment conducted at 190 ◦C for 5 min gave a pentose yield of 57%, with
only minor amounts of degradation compounds formed. Commercial cellulolytic enzymes were used to
hydrolyze the obtained fiber fractions after pretreatment at different water-insoluble... (More) - Sugarcane bagasse is a lignocellulosic residue obtained from sugarcane milling, and a potentially interesting
raw material that can be used for fuel ethanol production. In the present study, bagasse was
steam pretreated at temperatures between 180 and 205 ◦C, with holding times of 5–10 min using SO2
as a catalyst to determine conditions that provide a good recovery of pentoses and a suitable material
for enzymatic hydrolysis. Pretreatment conducted at 190 ◦C for 5 min gave a pentose yield of 57%, with
only minor amounts of degradation compounds formed. Commercial cellulolytic enzymes were used to
hydrolyze the obtained fiber fractions after pretreatment at different water-insoluble solid contents (2%,
5% and 8% WIS). The overall highest sugar yield achieved from bagasse was 87% at 2% WIS. Fermentation
tests were made on both the pentose-rich hemicellulose hydrolysate obtained from the pretreatment,
and the enzymatic hydrolysates obtained from the fiber fractions using the xylose-fermenting strain of
Saccharomyces cerevisiae TMB3400, as well as the natural xylose-utilizing yeast Pichia stipitis CBS 6054.
The pretreatment hydrolysates produced at 2% WIS as well as the enzymatic hydrolysates showed a
complete glucose fermentability indicating a low toxicity to the yeasts. The best xylose conversion (more
than 60%) was achieved by the strain TMB3400 at 2% WIS. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1978093
- author
- Carrasco, Cristhian LU ; Baudel, Henrique Macedo ; Sendelius, Johan ; Modig, Tobias LU ; Roslander, Christian LU ; Galbe, Mats LU ; Hahn-Hägerdal, Bärbel LU ; Zacchi, Guido LU and Lidén, Gunnar LU
- organization
- publishing date
- 2010
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Sugarcane bagasse Bioethanol Xylose Yeast Saccharomyces cerevisiae
- in
- Enzyme and Microbial Technology
- volume
- 46
- issue
- 2
- pages
- 64 - 73
- publisher
- Elsevier
- external identifiers
-
- wos:000274093600002
- scopus:71249111390
- ISSN
- 0141-0229
- DOI
- 10.1016/j.enzmictec.2009.10.016
- language
- English
- LU publication?
- yes
- id
- 5eb2df6f-c50b-467a-ba74-b24de5969060 (old id 1978093)
- date added to LUP
- 2016-04-01 10:16:31
- date last changed
- 2023-11-09 16:41:38
@article{5eb2df6f-c50b-467a-ba74-b24de5969060,
abstract = {{Sugarcane bagasse is a lignocellulosic residue obtained from sugarcane milling, and a potentially interesting<br/><br>
raw material that can be used for fuel ethanol production. In the present study, bagasse was<br/><br>
steam pretreated at temperatures between 180 and 205 ◦C, with holding times of 5–10 min using SO2<br/><br>
as a catalyst to determine conditions that provide a good recovery of pentoses and a suitable material<br/><br>
for enzymatic hydrolysis. Pretreatment conducted at 190 ◦C for 5 min gave a pentose yield of 57%, with<br/><br>
only minor amounts of degradation compounds formed. Commercial cellulolytic enzymes were used to<br/><br>
hydrolyze the obtained fiber fractions after pretreatment at different water-insoluble solid contents (2%,<br/><br>
5% and 8% WIS). The overall highest sugar yield achieved from bagasse was 87% at 2% WIS. Fermentation<br/><br>
tests were made on both the pentose-rich hemicellulose hydrolysate obtained from the pretreatment,<br/><br>
and the enzymatic hydrolysates obtained from the fiber fractions using the xylose-fermenting strain of<br/><br>
Saccharomyces cerevisiae TMB3400, as well as the natural xylose-utilizing yeast Pichia stipitis CBS 6054.<br/><br>
The pretreatment hydrolysates produced at 2% WIS as well as the enzymatic hydrolysates showed a<br/><br>
complete glucose fermentability indicating a low toxicity to the yeasts. The best xylose conversion (more<br/><br>
than 60%) was achieved by the strain TMB3400 at 2% WIS.}},
author = {{Carrasco, Cristhian and Baudel, Henrique Macedo and Sendelius, Johan and Modig, Tobias and Roslander, Christian and Galbe, Mats and Hahn-Hägerdal, Bärbel and Zacchi, Guido and Lidén, Gunnar}},
issn = {{0141-0229}},
keywords = {{Sugarcane bagasse
Bioethanol
Xylose
Yeast
Saccharomyces cerevisiae}},
language = {{eng}},
number = {{2}},
pages = {{64--73}},
publisher = {{Elsevier}},
series = {{Enzyme and Microbial Technology}},
title = {{SO2-catalyzed steam pretreatment and fermentation of enzymatically hydrolyzed sugarcane bagasse}},
url = {{http://dx.doi.org/10.1016/j.enzmictec.2009.10.016}},
doi = {{10.1016/j.enzmictec.2009.10.016}},
volume = {{46}},
year = {{2010}},
}