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Effect of Human Myotubes-Derived Media on Glucose-Stimulated Insulin Secretion

Mizgier, Maria L ; Cataldo, Luis R LU orcid ; Gutierrez, Juan ; Santos, José L ; Casas, Mariana ; Llanos, Paola ; Contreras-Ferrat, Ariel E ; Moro, Cedric ; Bouzakri, Karim and Galgani, Jose E (2017) In Journal of Diabetes Research 2017.
Abstract

Fasting to postprandial transition requires a tight adjustment of insulin secretion to its demand, so tissue (e.g., skeletal muscle) glucose supply is assured while hypo-/hyperglycemia are prevented. High muscle glucose disposal after meals is pivotal for adapting to increased glycemia and might drive insulin secretion through muscle-released factors (e.g., myokines). We hypothesized that insulin influences myokine secretion and then increases glucose-stimulated insulin secretion (GSIS). In conditioned media from human myotubes incubated with/without insulin (100 nmol/L) for 24 h, myokines were qualitatively and quantitatively characterized using an antibody-based array and ELISA-based technology, respectively. C57BL6/J mice islets and... (More)

Fasting to postprandial transition requires a tight adjustment of insulin secretion to its demand, so tissue (e.g., skeletal muscle) glucose supply is assured while hypo-/hyperglycemia are prevented. High muscle glucose disposal after meals is pivotal for adapting to increased glycemia and might drive insulin secretion through muscle-released factors (e.g., myokines). We hypothesized that insulin influences myokine secretion and then increases glucose-stimulated insulin secretion (GSIS). In conditioned media from human myotubes incubated with/without insulin (100 nmol/L) for 24 h, myokines were qualitatively and quantitatively characterized using an antibody-based array and ELISA-based technology, respectively. C57BL6/J mice islets and Wistar rat beta cells were incubated for 24 h with control and conditioned media from noninsulin- and insulin-treated myotubes prior to GSIS determination. Conditioned media from insulin-treated versus nontreated myotubes had higher RANTES but lower IL6, IL8, and MCP1 concentration. Qualitative analyses revealed that conditioned media from noninsulin- and insulin-treated myotubes expressed 32 and 23 out of 80 myokines, respectively. Islets incubated with conditioned media from noninsulin-treated myotubes had higher GSIS versus control islets (p< 0.05). Meanwhile, conditioned media from insulin-treated myotubes did not influence GSIS. In beta cells, GSIS was similar across conditions. In conclusion, factors being present in noninsulin-stimulated muscle cell-derived media appear to influence GSIS in mice islets.

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author
; ; ; ; ; ; ; ; and
publishing date
type
Contribution to journal
publication status
published
keywords
Animals, Chemokine CCL5, Culture Media, Conditioned, Glucose, Humans, Insulin, Insulin-Secreting Cells, Interleukin-6, Interleukin-8, Islets of Langerhans, Mice, Muscle Fibers, Skeletal, Rats, Rats, Wistar, Journal Article
in
Journal of Diabetes Research
volume
2017
article number
1328573
pages
9 pages
publisher
Hindawi Limited
external identifiers
  • scopus:85014284166
  • pmid:28286777
ISSN
2314-6753
DOI
10.1155/2017/1328573
language
English
LU publication?
no
id
199df4ae-35df-40ab-bd9a-b2dc048e5cbd
date added to LUP
2018-02-22 07:36:24
date last changed
2024-01-14 15:34:42
@article{199df4ae-35df-40ab-bd9a-b2dc048e5cbd,
  abstract     = {{<p>Fasting to postprandial transition requires a tight adjustment of insulin secretion to its demand, so tissue (e.g., skeletal muscle) glucose supply is assured while hypo-/hyperglycemia are prevented. High muscle glucose disposal after meals is pivotal for adapting to increased glycemia and might drive insulin secretion through muscle-released factors (e.g., myokines). We hypothesized that insulin influences myokine secretion and then increases glucose-stimulated insulin secretion (GSIS). In conditioned media from human myotubes incubated with/without insulin (100 nmol/L) for 24 h, myokines were qualitatively and quantitatively characterized using an antibody-based array and ELISA-based technology, respectively. C57BL6/J mice islets and Wistar rat beta cells were incubated for 24 h with control and conditioned media from noninsulin- and insulin-treated myotubes prior to GSIS determination. Conditioned media from insulin-treated versus nontreated myotubes had higher RANTES but lower IL6, IL8, and MCP1 concentration. Qualitative analyses revealed that conditioned media from noninsulin- and insulin-treated myotubes expressed 32 and 23 out of 80 myokines, respectively. Islets incubated with conditioned media from noninsulin-treated myotubes had higher GSIS versus control islets (p&lt; 0.05). Meanwhile, conditioned media from insulin-treated myotubes did not influence GSIS. In beta cells, GSIS was similar across conditions. In conclusion, factors being present in noninsulin-stimulated muscle cell-derived media appear to influence GSIS in mice islets.</p>}},
  author       = {{Mizgier, Maria L and Cataldo, Luis R and Gutierrez, Juan and Santos, José L and Casas, Mariana and Llanos, Paola and Contreras-Ferrat, Ariel E and Moro, Cedric and Bouzakri, Karim and Galgani, Jose E}},
  issn         = {{2314-6753}},
  keywords     = {{Animals; Chemokine CCL5; Culture Media, Conditioned; Glucose; Humans; Insulin; Insulin-Secreting Cells; Interleukin-6; Interleukin-8; Islets of Langerhans; Mice; Muscle Fibers, Skeletal; Rats; Rats, Wistar; Journal Article}},
  language     = {{eng}},
  publisher    = {{Hindawi Limited}},
  series       = {{Journal of Diabetes Research}},
  title        = {{Effect of Human Myotubes-Derived Media on Glucose-Stimulated Insulin Secretion}},
  url          = {{http://dx.doi.org/10.1155/2017/1328573}},
  doi          = {{10.1155/2017/1328573}},
  volume       = {{2017}},
  year         = {{2017}},
}