Microfluidic separation of parasites and parasite-infected cells from blood for the diagnosis of leishmaniasis
(2016) 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016 p.244-245- Abstract
Microfluidic techniques were applied to the separation of parasite and parasite-infected cells from blood to facilitate the detection of leishmaniasis, a disease representing a high burden in the developing world and for which new diagnostic tools are urgently needed. Leishmania mexicana promastigotes were successfully separated from red blood cells in a deterministic lateral displacement device. The mechanical properties of macrophages infected with L. mexicana were investigated using real-time deformability cytometry. In the early stage, we find that macrophages deform less than the control. The trend is reversed four days post infection while we see a continuous increase in cell size after parasitization.
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1baebc20-7549-416c-a893-3ff713d8aca9
- author
- Regnault, C. ; Punyani, K. LU ; Holm, S. LU ; Beech, J. P. LU ; Otto, Marcia C. de Oliveira ; Herold, Kevan C ; Herbig, M. ; Guck, J. ; Tegenfeldt, J. O. LU and Barrett, Michael P
- organization
- publishing date
- 2016
- type
- Chapter in Book/Report/Conference proceeding
- publication status
- published
- subject
- keywords
- Deformability, Diagnosis, Leishmaniasis, Sorting
- host publication
- 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016
- pages
- 2 pages
- publisher
- Chemical and Biological Microsystems Society
- conference name
- 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016
- conference location
- Dublin, Ireland
- conference dates
- 2016-10-09 - 2016-10-13
- external identifiers
-
- scopus:85014166507
- ISBN
- 9780979806490
- language
- English
- LU publication?
- yes
- id
- 1baebc20-7549-416c-a893-3ff713d8aca9
- date added to LUP
- 2017-03-16 10:29:21
- date last changed
- 2023-10-05 01:47:50
@inproceedings{1baebc20-7549-416c-a893-3ff713d8aca9, abstract = {{<p>Microfluidic techniques were applied to the separation of parasite and parasite-infected cells from blood to facilitate the detection of leishmaniasis, a disease representing a high burden in the developing world and for which new diagnostic tools are urgently needed. Leishmania mexicana promastigotes were successfully separated from red blood cells in a deterministic lateral displacement device. The mechanical properties of macrophages infected with L. mexicana were investigated using real-time deformability cytometry. In the early stage, we find that macrophages deform less than the control. The trend is reversed four days post infection while we see a continuous increase in cell size after parasitization.</p>}}, author = {{Regnault, C. and Punyani, K. and Holm, S. and Beech, J. P. and Otto, Marcia C. de Oliveira and Herold, Kevan C and Herbig, M. and Guck, J. and Tegenfeldt, J. O. and Barrett, Michael P}}, booktitle = {{20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016}}, isbn = {{9780979806490}}, keywords = {{Deformability; Diagnosis; Leishmaniasis; Sorting}}, language = {{eng}}, pages = {{244--245}}, publisher = {{Chemical and Biological Microsystems Society}}, title = {{Microfluidic separation of parasites and parasite-infected cells from blood for the diagnosis of leishmaniasis}}, year = {{2016}}, }