Generation of a conditionally null allele of the laminin alpha 1 gene
(2005) In Genesis: The Journal of Genetics and Development 43(2). p.59-70- Abstract
- Laminins are heterotrimeric glycoproteins of the basement membranes. Laminin 1 (alpha 1, beta 1, gamma 1) is the major laminin expressed during early mouse embryogenesis. To gain access, to the physiological function of laminin alpha 1 chain, we developed a conditionally null allele of its encoding gene (Lama1) using the cre/loxP system. Floxed-allele-carrying mice (Lama1(flox/flox)) display no overt phenotype. Lama1(flox/flox) mice were crossed with transgenic deleter mice (CMV-Cre) to generate Lama1-deficient mice (Lama1(Delta/Delta)). Lama1(Delta/Delta) embryos die during the early postimplantation period after embryonic day 6.5. They lack Reichert's membrane, an extraembryonic basement membrane in which laminin all is normally highly... (More)
- Laminins are heterotrimeric glycoproteins of the basement membranes. Laminin 1 (alpha 1, beta 1, gamma 1) is the major laminin expressed during early mouse embryogenesis. To gain access, to the physiological function of laminin alpha 1 chain, we developed a conditionally null allele of its encoding gene (Lama1) using the cre/loxP system. Floxed-allele-carrying mice (Lama1(flox/flox)) display no overt phenotype. Lama1(flox/flox) mice were crossed with transgenic deleter mice (CMV-Cre) to generate Lama1-deficient mice (Lama1(Delta/Delta)). Lama1(Delta/Delta) embryos die during the early postimplantation period after embryonic day 6.5. They lack Reichert's membrane, an extraembryonic basement membrane in which laminin all is normally highly expressed. In parallel, Lama1(Delta/Delta) embryos display 1) parietal and visceral endoderm differentiation defects with altered expression of cytokeratin 19 and GATA4, respectively, and 2) an induction of apoptosis. This new mouse model is of particular interest as it will allow time- and tissue-specific inactivation of the Lamal gene in various organs. genesis 43:59-70, 2005. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/214239
- author
- Alpy, F ; Jivkov, I ; Sorokin, Lydia LU ; Klein, A ; Arnold, C ; Huss, Y ; Kedinger, M ; Simon-Assmann, R and Lefebvre, O
- organization
- publishing date
- 2005
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Reichert's membrane, development, apoptosis, GATA4, conditional inactivation, Lama1 genes, knockout, basement membrane
- in
- Genesis: The Journal of Genetics and Development
- volume
- 43
- issue
- 2
- pages
- 59 - 70
- publisher
- John Wiley & Sons Inc.
- external identifiers
-
- wos:000233060100002
- pmid:16100707
- scopus:27744522715
- ISSN
- 1526-954X
- DOI
- 10.1002/gene.20154
- language
- English
- LU publication?
- yes
- additional info
- The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Pathology, (Lund) (013030000)
- id
- bac70cbc-4854-4b7d-ba81-91452a948b42 (old id 214239)
- date added to LUP
- 2016-04-01 12:27:45
- date last changed
- 2022-03-29 01:13:34
@article{bac70cbc-4854-4b7d-ba81-91452a948b42,
abstract = {{Laminins are heterotrimeric glycoproteins of the basement membranes. Laminin 1 (alpha 1, beta 1, gamma 1) is the major laminin expressed during early mouse embryogenesis. To gain access, to the physiological function of laminin alpha 1 chain, we developed a conditionally null allele of its encoding gene (Lama1) using the cre/loxP system. Floxed-allele-carrying mice (Lama1(flox/flox)) display no overt phenotype. Lama1(flox/flox) mice were crossed with transgenic deleter mice (CMV-Cre) to generate Lama1-deficient mice (Lama1(Delta/Delta)). Lama1(Delta/Delta) embryos die during the early postimplantation period after embryonic day 6.5. They lack Reichert's membrane, an extraembryonic basement membrane in which laminin all is normally highly expressed. In parallel, Lama1(Delta/Delta) embryos display 1) parietal and visceral endoderm differentiation defects with altered expression of cytokeratin 19 and GATA4, respectively, and 2) an induction of apoptosis. This new mouse model is of particular interest as it will allow time- and tissue-specific inactivation of the Lamal gene in various organs. genesis 43:59-70, 2005.}},
author = {{Alpy, F and Jivkov, I and Sorokin, Lydia and Klein, A and Arnold, C and Huss, Y and Kedinger, M and Simon-Assmann, R and Lefebvre, O}},
issn = {{1526-954X}},
keywords = {{Reichert's membrane; development; apoptosis; GATA4; conditional inactivation; Lama1 genes; knockout; basement membrane}},
language = {{eng}},
number = {{2}},
pages = {{59--70}},
publisher = {{John Wiley & Sons Inc.}},
series = {{Genesis: The Journal of Genetics and Development}},
title = {{Generation of a conditionally null allele of the laminin alpha 1 gene}},
url = {{http://dx.doi.org/10.1002/gene.20154}},
doi = {{10.1002/gene.20154}},
volume = {{43}},
year = {{2005}},
}