Pathogenic Autoreactive B Cells Are Not Negatively Selected toward Matrix Protein Collagen II.
(2011) In Journal of immunology 187. p.4451-4458- Abstract
- We have addressed the importance of B cell tolerance to collagen type II, a matrix protein, which is a target in rheumatoid arthritis (RA) and its mouse models. We generated a germline-encoded anti-collagen type II (CII) IgH replacement anti-C1 B cell mouse strain (ACB) to investigate how B cell tolerance to CII, a matrix protein, is subverted and to further understand pathogenesis of RA. Phenotypic analysis revealed that CII-specific B cells were surprisingly neither deleted nor anergized. Instead, they were readily detected in all lymphoid organs. Spontaneously produced autoantibodies could bind directly to cartilage surface without detectable pathology. However, exaggerated arthritis was seen after injection of anti-CII Abs specific for... (More)
- We have addressed the importance of B cell tolerance to collagen type II, a matrix protein, which is a target in rheumatoid arthritis (RA) and its mouse models. We generated a germline-encoded anti-collagen type II (CII) IgH replacement anti-C1 B cell mouse strain (ACB) to investigate how B cell tolerance to CII, a matrix protein, is subverted and to further understand pathogenesis of RA. Phenotypic analysis revealed that CII-specific B cells were surprisingly neither deleted nor anergized. Instead, they were readily detected in all lymphoid organs. Spontaneously produced autoantibodies could bind directly to cartilage surface without detectable pathology. However, exaggerated arthritis was seen after injection of anti-CII Abs specific for other epitopes. In addition, Abs from CII-specific hybridomas generated from ACB mice induced arthritis. Interestingly, IgH/L chain sequence data in B cell hybridomas revealed a lack of somatic mutations in autoreactive B cells. The ACB model provides the first possibility, to our knowledge, to study B cell tolerance to a matrix protein, and the observations made in the study could not be predicted from previous models. B cell-reactive epitopes on CII are largely shared between human RA and rodent CII-induced arthritis; this study, therefore, has important implications for further understanding of pathological processes in autoimmune diseases like RA. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/2168656
- author
- Cao, Duojia ; Khmaladze, Ia ; Jia, Hongwei ; Bajtner, Estelle ; Kutty Selva, Nandakumar ; Blom, Thomas LU ; Mo, John A and Holmdahl, Rikard
- organization
- publishing date
- 2011
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Journal of immunology
- volume
- 187
- pages
- 4451 - 4458
- publisher
- American Association of Immunologists
- external identifiers
-
- wos:000296496000010
- pmid:21940677
- scopus:80555154914
- pmid:21940677
- ISSN
- 1550-6606
- DOI
- 10.4049/jimmunol.1101378
- language
- English
- LU publication?
- yes
- additional info
- The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Faculty of Medicine (000022000), BMC Biomedical Centre (0130322000), Medical Inflammation Research (013212019)
- id
- a57da59c-2e9d-4335-9092-229c351a8d48 (old id 2168656)
- alternative location
- http://www.ncbi.nlm.nih.gov/pubmed/21940677?dopt=Abstract
- date added to LUP
- 2016-04-04 09:43:31
- date last changed
- 2023-09-06 03:19:43
@article{a57da59c-2e9d-4335-9092-229c351a8d48,
abstract = {{We have addressed the importance of B cell tolerance to collagen type II, a matrix protein, which is a target in rheumatoid arthritis (RA) and its mouse models. We generated a germline-encoded anti-collagen type II (CII) IgH replacement anti-C1 B cell mouse strain (ACB) to investigate how B cell tolerance to CII, a matrix protein, is subverted and to further understand pathogenesis of RA. Phenotypic analysis revealed that CII-specific B cells were surprisingly neither deleted nor anergized. Instead, they were readily detected in all lymphoid organs. Spontaneously produced autoantibodies could bind directly to cartilage surface without detectable pathology. However, exaggerated arthritis was seen after injection of anti-CII Abs specific for other epitopes. In addition, Abs from CII-specific hybridomas generated from ACB mice induced arthritis. Interestingly, IgH/L chain sequence data in B cell hybridomas revealed a lack of somatic mutations in autoreactive B cells. The ACB model provides the first possibility, to our knowledge, to study B cell tolerance to a matrix protein, and the observations made in the study could not be predicted from previous models. B cell-reactive epitopes on CII are largely shared between human RA and rodent CII-induced arthritis; this study, therefore, has important implications for further understanding of pathological processes in autoimmune diseases like RA.}},
author = {{Cao, Duojia and Khmaladze, Ia and Jia, Hongwei and Bajtner, Estelle and Kutty Selva, Nandakumar and Blom, Thomas and Mo, John A and Holmdahl, Rikard}},
issn = {{1550-6606}},
language = {{eng}},
pages = {{4451--4458}},
publisher = {{American Association of Immunologists}},
series = {{Journal of immunology}},
title = {{Pathogenic Autoreactive B Cells Are Not Negatively Selected toward Matrix Protein Collagen II.}},
url = {{http://dx.doi.org/10.4049/jimmunol.1101378}},
doi = {{10.4049/jimmunol.1101378}},
volume = {{187}},
year = {{2011}},
}