Preparation of hydrophilic poly(3-hydroxybutyrate) macroporous scaffolds through enzyme-mediated modifications
(2011) In Journal of Bioactive and Compatible Polymers 26(5). p.452-463- Abstract
- Scaffolds made from poly(3-hydroxybutyrate) were prepared by thermally induced phase separation from solutions in dioxane at temperatures above (23 degrees C) or below the freezing point of the solvent. At 23 degrees C, gelation occured with nano-fibrous network formation. The scaffolds prepared at -12 degrees C and -25 degrees C exhibited a highly porous morphology, with pores in the range of 3-25 mu m, caused by freezing of dioxane. The macroporous scaffolds, prepared at -12 degrees C, were treated with lipase to generate functional groups to which gelatin and glucosamine, respectively, were chemically coupled. The modified scaffolds had lower molecular weight, higher water content, lower melting temperature, and enthalpy. Cultivation of... (More)
- Scaffolds made from poly(3-hydroxybutyrate) were prepared by thermally induced phase separation from solutions in dioxane at temperatures above (23 degrees C) or below the freezing point of the solvent. At 23 degrees C, gelation occured with nano-fibrous network formation. The scaffolds prepared at -12 degrees C and -25 degrees C exhibited a highly porous morphology, with pores in the range of 3-25 mu m, caused by freezing of dioxane. The macroporous scaffolds, prepared at -12 degrees C, were treated with lipase to generate functional groups to which gelatin and glucosamine, respectively, were chemically coupled. The modified scaffolds had lower molecular weight, higher water content, lower melting temperature, and enthalpy. Cultivation of human embryonic fibroblasts on the macroporous scaffolds confirmed that the cells proliferated and adhered to the materials. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/2183487
- author
- Guzmán, Daniel LU ; Kirsebom, Harald LU ; Solano, Carlos LU ; Quillaguaman, Jorge and Hatti-Kaul, Rajni LU
- organization
- publishing date
- 2011
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- biopolyester, poly(3-hydroxybutyrate), thermally induced phase, separation, lipase, glucosamine, gelatin, carbodiimide coupling
- in
- Journal of Bioactive and Compatible Polymers
- volume
- 26
- issue
- 5
- pages
- 452 - 463
- publisher
- SAGE Publications
- external identifiers
-
- wos:000294816100002
- scopus:80052733250
- ISSN
- 0883-9115
- DOI
- 10.1177/0883911511419970
- language
- English
- LU publication?
- yes
- additional info
- The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Biotechnology (LTH) (011001037), Organic chemistry (S/LTH) (011001240)
- id
- d78cabab-b395-4ead-9942-c98398ce08c4 (old id 2183487)
- date added to LUP
- 2016-04-01 10:51:59
- date last changed
- 2022-01-26 03:12:10
@article{d78cabab-b395-4ead-9942-c98398ce08c4, abstract = {{Scaffolds made from poly(3-hydroxybutyrate) were prepared by thermally induced phase separation from solutions in dioxane at temperatures above (23 degrees C) or below the freezing point of the solvent. At 23 degrees C, gelation occured with nano-fibrous network formation. The scaffolds prepared at -12 degrees C and -25 degrees C exhibited a highly porous morphology, with pores in the range of 3-25 mu m, caused by freezing of dioxane. The macroporous scaffolds, prepared at -12 degrees C, were treated with lipase to generate functional groups to which gelatin and glucosamine, respectively, were chemically coupled. The modified scaffolds had lower molecular weight, higher water content, lower melting temperature, and enthalpy. Cultivation of human embryonic fibroblasts on the macroporous scaffolds confirmed that the cells proliferated and adhered to the materials.}}, author = {{Guzmán, Daniel and Kirsebom, Harald and Solano, Carlos and Quillaguaman, Jorge and Hatti-Kaul, Rajni}}, issn = {{0883-9115}}, keywords = {{biopolyester; poly(3-hydroxybutyrate); thermally induced phase; separation; lipase; glucosamine; gelatin; carbodiimide coupling}}, language = {{eng}}, number = {{5}}, pages = {{452--463}}, publisher = {{SAGE Publications}}, series = {{Journal of Bioactive and Compatible Polymers}}, title = {{Preparation of hydrophilic poly(3-hydroxybutyrate) macroporous scaffolds through enzyme-mediated modifications}}, url = {{http://dx.doi.org/10.1177/0883911511419970}}, doi = {{10.1177/0883911511419970}}, volume = {{26}}, year = {{2011}}, }