Serine/arginine-rich protein 30c activates human papillomavirus type 16 L1 mRNA expression via a bimodal mechanism
(2011) In Journal of General Virology 92. p.2411-2421- Abstract
- Two splice sites on the human papillomavirus type 16 (HPV-16) genome are used exclusively by the late capsid protein L1 mRNAs: SD3632 and SA5639. These splice sites are suppressed in mitotic cells. This study showed that serine/arginine-rich protein 30c (SRp30c), also named SFRS9, activated both SD3632 and SA5639 and induced production of L1 mRNA. Activation of HPV-16 L1 mRNA splicing by SRp30c required an intact arginine/serine-repeat (RS) domain of SRp30c. In addition to this effect, SRp30c could enhance L1 mRNA production indirectly by inhibiting the early 3'-splice site SA3358, which competed with the late 3'-splice site SA5639. SRp30c bound directly to sequences downstream of SA3358, suggesting that SRp30c inhibited the enhancer at... (More)
- Two splice sites on the human papillomavirus type 16 (HPV-16) genome are used exclusively by the late capsid protein L1 mRNAs: SD3632 and SA5639. These splice sites are suppressed in mitotic cells. This study showed that serine/arginine-rich protein 30c (SRp30c), also named SFRS9, activated both SD3632 and SA5639 and induced production of L1 mRNA. Activation of HPV-16 L1 mRNA splicing by SRp30c required an intact arginine/serine-repeat (RS) domain of SRp30c. In addition to this effect, SRp30c could enhance L1 mRNA production indirectly by inhibiting the early 3'-splice site SA3358, which competed with the late 3'-splice site SA5639. SRp30c bound directly to sequences downstream of SA3358, suggesting that SRp30c inhibited the enhancer at SA3358 and caused a redirection of splicing to the late 3'-splice site SA5639. This inhibitory effect of SRp30c was independent of its RS domain. These results suggest that SRp30c can activate HPV-16 L1 mRNA expression via a bimodal mechanism: directly by stimulating splicing to late splice sites and indirectly by inhibiting competing early splice sites. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/2208232
- author
- Somberg, Monika ; Li, Xiaoze LU ; Johansson, Cecilia ; Orru, Beatrice ; Chang, Roger ; Rush, Margaret ; Fay, Joanna ; Ryan, Fergus and Schwartz, Stefan LU
- organization
- publishing date
- 2011
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Journal of General Virology
- volume
- 92
- pages
- 2411 - 2421
- publisher
- Microbiology Society
- external identifiers
-
- wos:000295856000022
- scopus:80052922847
- pmid:21697349
- ISSN
- 1465-2099
- DOI
- 10.1099/vir.0.033183-0
- language
- English
- LU publication?
- yes
- id
- cda6a9d8-ce28-462f-b760-0198be333fc1 (old id 2208232)
- date added to LUP
- 2016-04-01 13:56:39
- date last changed
- 2022-03-14 02:43:48
@article{cda6a9d8-ce28-462f-b760-0198be333fc1, abstract = {{Two splice sites on the human papillomavirus type 16 (HPV-16) genome are used exclusively by the late capsid protein L1 mRNAs: SD3632 and SA5639. These splice sites are suppressed in mitotic cells. This study showed that serine/arginine-rich protein 30c (SRp30c), also named SFRS9, activated both SD3632 and SA5639 and induced production of L1 mRNA. Activation of HPV-16 L1 mRNA splicing by SRp30c required an intact arginine/serine-repeat (RS) domain of SRp30c. In addition to this effect, SRp30c could enhance L1 mRNA production indirectly by inhibiting the early 3'-splice site SA3358, which competed with the late 3'-splice site SA5639. SRp30c bound directly to sequences downstream of SA3358, suggesting that SRp30c inhibited the enhancer at SA3358 and caused a redirection of splicing to the late 3'-splice site SA5639. This inhibitory effect of SRp30c was independent of its RS domain. These results suggest that SRp30c can activate HPV-16 L1 mRNA expression via a bimodal mechanism: directly by stimulating splicing to late splice sites and indirectly by inhibiting competing early splice sites.}}, author = {{Somberg, Monika and Li, Xiaoze and Johansson, Cecilia and Orru, Beatrice and Chang, Roger and Rush, Margaret and Fay, Joanna and Ryan, Fergus and Schwartz, Stefan}}, issn = {{1465-2099}}, language = {{eng}}, pages = {{2411--2421}}, publisher = {{Microbiology Society}}, series = {{Journal of General Virology}}, title = {{Serine/arginine-rich protein 30c activates human papillomavirus type 16 L1 mRNA expression via a bimodal mechanism}}, url = {{http://dx.doi.org/10.1099/vir.0.033183-0}}, doi = {{10.1099/vir.0.033183-0}}, volume = {{92}}, year = {{2011}}, }