Helicobacter species and gut bacterial DNA in Meckel's diverticulum and the appendix.
(2011) In World Journal of Gastroenterology 17(36). p.4104-4108- Abstract
- AIM: To analyse the possible association of various Helicobacter species and certain common gut bacteria in patients with Meckel's diverticulum and appendicitis.
METHODS:
A nested-polymerase chain reaction (PCR), specific to 16S rRNA of the Helicobacter genus, was performed on paraffin embedded samples, 50 with acute appendicitis, 50 normal appendixes, and 33 Meckel's diverticulum with gastric heterotopia and/or ulcer. Helicobacter genus positive samples were sequenced for species identification. All samples were also analysed for certain gut bacteria by PCR.
RESULTS:
Helicobacter pullorum DNA was found in one out of 33 cases and Enterobacteria in two cases of Meckel's diverticulum.... (More) - AIM: To analyse the possible association of various Helicobacter species and certain common gut bacteria in patients with Meckel's diverticulum and appendicitis.
METHODS:
A nested-polymerase chain reaction (PCR), specific to 16S rRNA of the Helicobacter genus, was performed on paraffin embedded samples, 50 with acute appendicitis, 50 normal appendixes, and 33 Meckel's diverticulum with gastric heterotopia and/or ulcer. Helicobacter genus positive samples were sequenced for species identification. All samples were also analysed for certain gut bacteria by PCR.
RESULTS:
Helicobacter pullorum DNA was found in one out of 33 cases and Enterobacteria in two cases of Meckel's diverticulum. Helicobacter pylori (H. pylori) was found in three, Enterobacter in 18, and Bacteroides in 19 out of 100 appendix samples by PCR. Enterococcus was not found in any MD or appendix samples. All H. pylori positive cases were from normal appendixes.
CONCLUSION:
Helicobacter is not an etiological agent in the pathogenesis of symptomatic Meckel's diverticulum or in acute appendicitis. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/2221386
- author
- Karagin, Peren H ; Stenram, Unne LU ; Wadström, Torkel LU and Ljungh, Åsa LU
- organization
- publishing date
- 2011
- type
- Contribution to journal
- publication status
- published
- subject
- in
- World Journal of Gastroenterology
- volume
- 17
- issue
- 36
- pages
- 4104 - 4108
- publisher
- WJG Press
- external identifiers
-
- wos:000296871300007
- pmid:22039324
- scopus:80155191167
- pmid:22039324
- ISSN
- 1007-9327
- DOI
- 10.3748/wjg.v17.i36.4104
- language
- English
- LU publication?
- yes
- additional info
- The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Pathology, (Lund) (013030000), Division of Medical Microbiology (013250400)
- id
- ef9b9a71-05c3-4f34-afbf-9f249463e927 (old id 2221386)
- alternative location
- http://www.ncbi.nlm.nih.gov/pubmed/22039324?dopt=Abstract
- date added to LUP
- 2016-04-04 08:47:22
- date last changed
- 2022-01-29 03:59:07
@article{ef9b9a71-05c3-4f34-afbf-9f249463e927,
abstract = {{AIM: To analyse the possible association of various Helicobacter species and certain common gut bacteria in patients with Meckel's diverticulum and appendicitis.<br/><br>
<br/><br>
METHODS:<br/><br>
A nested-polymerase chain reaction (PCR), specific to 16S rRNA of the Helicobacter genus, was performed on paraffin embedded samples, 50 with acute appendicitis, 50 normal appendixes, and 33 Meckel's diverticulum with gastric heterotopia and/or ulcer. Helicobacter genus positive samples were sequenced for species identification. All samples were also analysed for certain gut bacteria by PCR.<br/><br>
<br/><br>
RESULTS:<br/><br>
Helicobacter pullorum DNA was found in one out of 33 cases and Enterobacteria in two cases of Meckel's diverticulum. Helicobacter pylori (H. pylori) was found in three, Enterobacter in 18, and Bacteroides in 19 out of 100 appendix samples by PCR. Enterococcus was not found in any MD or appendix samples. All H. pylori positive cases were from normal appendixes.<br/><br>
<br/><br>
CONCLUSION:<br/><br>
Helicobacter is not an etiological agent in the pathogenesis of symptomatic Meckel's diverticulum or in acute appendicitis.}},
author = {{Karagin, Peren H and Stenram, Unne and Wadström, Torkel and Ljungh, Åsa}},
issn = {{1007-9327}},
language = {{eng}},
number = {{36}},
pages = {{4104--4108}},
publisher = {{WJG Press}},
series = {{World Journal of Gastroenterology}},
title = {{Helicobacter species and gut bacterial DNA in Meckel's diverticulum and the appendix.}},
url = {{http://dx.doi.org/10.3748/wjg.v17.i36.4104}},
doi = {{10.3748/wjg.v17.i36.4104}},
volume = {{17}},
year = {{2011}},
}