Labeling plasmin with technetium-99m for scintigraphic localization of thrombi
Persson, Bertil R.R. LU
and Darte, Lennart
LU
(1977)
In International Journal of Applied Radiation and Isotopes
28(1-2).
p.97-104
- Abstract
A detailed study has been made of the method for labeling plasmin (NOVO Indstri A/S Denmark) with 99mTc in order to prepare a radioactive indicator for early scintigraphic visualization of thrombi and tumors. The best method found for preparing 99mTc-plasmic involved the reduction of 2.5 ml 99mTc-pertechnetate solution with 0.5 ml of 4 mM SnCl2, 2 M NaCl and 70 mM HCl. This mixture was then added to 5 mg of plasmin to give a final pH of about 2. After 60 min of reaction the labeling efficiency was 80-90% as determined by gel chromatography column-scanning. The labeling kinetics and influence of pH, concentration of SnCl2, NaCl, plasmin and lysine were studied. The enzymatic activity... (More)
A detailed study has been made of the method for labeling plasmin (NOVO Indstri A/S Denmark) with 99mTc in order to prepare a radioactive indicator for early scintigraphic visualization of thrombi and tumors. The best method found for preparing 99mTc-plasmic involved the reduction of 2.5 ml 99mTc-pertechnetate solution with 0.5 ml of 4 mM SnCl2, 2 M NaCl and 70 mM HCl. This mixture was then added to 5 mg of plasmin to give a final pH of about 2. After 60 min of reaction the labeling efficiency was 80-90% as determined by gel chromatography column-scanning. The labeling kinetics and influence of pH, concentration of SnCl2, NaCl, plasmin and lysine were studied. The enzymatic activity of plasmin was reduced by less than 15% by the labeling process. Preliminary experiments in rabbits with artificially induced thrombi indicate accumulation of 99mTc activity in the same areas as 125I-fibrinogen after the administration of 99mTc-plasmin.
(Less)
- author
- Persson, Bertil R.R.
LU
and Darte, Lennart
LU
- organization
- publishing date
- 1977-01-01
- type
- Contribution to journal
- publication status
- published
- in
- International Journal of Applied Radiation and Isotopes
- volume
- 28
- issue
- 1-2
- pages
- 8 pages
- publisher
- Elsevier
- external identifiers
-
- pmid:140151
- scopus:0017367663
- ISSN
- 0020-708X
- DOI
- 10.1016/0020-708X(77)90163-6
- language
- English
- LU publication?
- yes
- id
- 222ff1e2-4211-40af-9ca7-ad69d496ac9c
- date added to LUP
- 2020-08-30 21:02:53
- date last changed
- 2024-01-02 17:28:10
@article{222ff1e2-4211-40af-9ca7-ad69d496ac9c,
abstract = {{<p>A detailed study has been made of the method for labeling plasmin (NOVO Indstri A/S Denmark) with <sup>99m</sup>Tc in order to prepare a radioactive indicator for early scintigraphic visualization of thrombi and tumors. The best method found for preparing <sup>99m</sup>Tc-plasmic involved the reduction of 2.5 ml <sup>99m</sup>Tc-pertechnetate solution with 0.5 ml of 4 mM SnCl<sub>2</sub>, 2 M NaCl and 70 mM HCl. This mixture was then added to 5 mg of plasmin to give a final pH of about 2. After 60 min of reaction the labeling efficiency was 80-90% as determined by gel chromatography column-scanning. The labeling kinetics and influence of pH, concentration of SnCl<sub>2</sub>, NaCl, plasmin and lysine were studied. The enzymatic activity of plasmin was reduced by less than 15% by the labeling process. Preliminary experiments in rabbits with artificially induced thrombi indicate accumulation of <sup>99m</sup>Tc activity in the same areas as <sup>125</sup>I-fibrinogen after the administration of <sup>99m</sup>Tc-plasmin.</p>}},
author = {{Persson, Bertil R.R. and Darte, Lennart}},
issn = {{0020-708X}},
language = {{eng}},
month = {{01}},
number = {{1-2}},
pages = {{97--104}},
publisher = {{Elsevier}},
series = {{International Journal of Applied Radiation and Isotopes}},
title = {{Labeling plasmin with technetium-99m for scintigraphic localization of thrombi}},
url = {{http://dx.doi.org/10.1016/0020-708X(77)90163-6}},
doi = {{10.1016/0020-708X(77)90163-6}},
volume = {{28}},
year = {{1977}},
}