Superior neovascularization and muscle regeneration in ischemic skeletal muscles following VEGF gene transfer by rAAV1 pseudotyped vectors
(2005) In Biochemical and Biophysical Research Communications 336(1). p.287-298- Abstract
- Recombinant adeno-associated virus serotype 2 (rAAV2) vector has been widely employed for gene therapy. Recent progress suggests that the new serotypes of AAV showed a better performance than did AAV2 in normal tissues. Here, we evaluate the potential role of human vascular endothelial growth factor (VEGF) gene transfer using rAAV vector pseudotyped with serotype I capsid proteins (rAAV1) in the treatment of muscle ischemia. In ischemic skeletal muscles, the rAAV1-LacZ vector allowed higher level, broader distribution, and long-lasting gene expression compared with the rAAV2-LacZ vector. Muscle VEGF165 production following the rAAV1-VEGF165 vector injection was 5-10 times higher than that following the rAAV2-VEGF165 vector injection.... (More)
- Recombinant adeno-associated virus serotype 2 (rAAV2) vector has been widely employed for gene therapy. Recent progress suggests that the new serotypes of AAV showed a better performance than did AAV2 in normal tissues. Here, we evaluate the potential role of human vascular endothelial growth factor (VEGF) gene transfer using rAAV vector pseudotyped with serotype I capsid proteins (rAAV1) in the treatment of muscle ischemia. In ischemic skeletal muscles, the rAAV1-LacZ vector allowed higher level, broader distribution, and long-lasting gene expression compared with the rAAV2-LacZ vector. Muscle VEGF165 production following the rAAV1-VEGF165 vector injection was 5-10 times higher than that following the rAAV2-VEGF165 vector injection. VEGF165 production mediated by the rAAV1-VEGF165 vector stimulated a large set of neovascularization with relatively mature vascular structures and enhanced muscle regeneration in the ischemic skeletal muscles. Thus, the rAAV1-NEGF165 vector mediated gene transfer may be a therapeutic approach to peripheral vascular diseases. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/223798
- author
- organization
- publishing date
- 2005
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- endothelial growth factor, vascular, gene delivery, serotype 1, adeno-associated virus, muscle, ischemia
- in
- Biochemical and Biophysical Research Communications
- volume
- 336
- issue
- 1
- pages
- 287 - 298
- publisher
- Elsevier
- external identifiers
-
- pmid:16129416
- wos:000231941500042
- scopus:24344431602
- ISSN
- 1090-2104
- DOI
- 10.1016/j.bbrc.2005.08.066
- language
- English
- LU publication?
- yes
- id
- 6ee7e7d8-898d-4d53-897d-abfa008a71e0 (old id 223798)
- date added to LUP
- 2016-04-01 15:26:52
- date last changed
- 2022-01-28 05:21:42
@article{6ee7e7d8-898d-4d53-897d-abfa008a71e0, abstract = {{Recombinant adeno-associated virus serotype 2 (rAAV2) vector has been widely employed for gene therapy. Recent progress suggests that the new serotypes of AAV showed a better performance than did AAV2 in normal tissues. Here, we evaluate the potential role of human vascular endothelial growth factor (VEGF) gene transfer using rAAV vector pseudotyped with serotype I capsid proteins (rAAV1) in the treatment of muscle ischemia. In ischemic skeletal muscles, the rAAV1-LacZ vector allowed higher level, broader distribution, and long-lasting gene expression compared with the rAAV2-LacZ vector. Muscle VEGF165 production following the rAAV1-VEGF165 vector injection was 5-10 times higher than that following the rAAV2-VEGF165 vector injection. VEGF165 production mediated by the rAAV1-VEGF165 vector stimulated a large set of neovascularization with relatively mature vascular structures and enhanced muscle regeneration in the ischemic skeletal muscles. Thus, the rAAV1-NEGF165 vector mediated gene transfer may be a therapeutic approach to peripheral vascular diseases.}}, author = {{Yan, H and Guo, YH and Zhang, P and Zu, LY and Dong, XY and Chen, L and Tian, JW and Fan, Xiaolong and Wang, NP and Wu, XB and Gao, W}}, issn = {{1090-2104}}, keywords = {{endothelial growth factor; vascular; gene delivery; serotype 1; adeno-associated virus; muscle; ischemia}}, language = {{eng}}, number = {{1}}, pages = {{287--298}}, publisher = {{Elsevier}}, series = {{Biochemical and Biophysical Research Communications}}, title = {{Superior neovascularization and muscle regeneration in ischemic skeletal muscles following VEGF gene transfer by rAAV1 pseudotyped vectors}}, url = {{http://dx.doi.org/10.1016/j.bbrc.2005.08.066}}, doi = {{10.1016/j.bbrc.2005.08.066}}, volume = {{336}}, year = {{2005}}, }