Guiding molecular motors with nano-imprinted structures
(2005) In Japanese Journal of Applied Physics 44(5A). p.3337-3340- Abstract
- This work, for the first time, demonstrates that nano-imprinted samples, with 100 nm wide polymer lines, can act as guides for molecular motors consisting of motor proteins actin and myosin. The motor protein function was characterized using fluorescence microscopy and compared to actomyosin motility on non-structured nitrocellulose surfaces. Our results open for further use of the nano-imprint technique in the production of disposable chips for bio-nanotechnological applications and miniaturized biological test systems. We discuss how the nano-imprinted motor protein assay system may be optimized and also how it compares to previously tested assay systems involving low-resolution UV-lithography and low throughput but high-resolution... (More)
- This work, for the first time, demonstrates that nano-imprinted samples, with 100 nm wide polymer lines, can act as guides for molecular motors consisting of motor proteins actin and myosin. The motor protein function was characterized using fluorescence microscopy and compared to actomyosin motility on non-structured nitrocellulose surfaces. Our results open for further use of the nano-imprint technique in the production of disposable chips for bio-nanotechnological applications and miniaturized biological test systems. We discuss how the nano-imprinted motor protein assay system may be optimized and also how it compares to previously tested assay systems involving low-resolution UV-lithography and low throughput but high-resolution electron beam lithography. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/224139
- author
- Bunk, Richard LU ; Carlberg, Patrick LU ; Mansson, A ; Nicholls, IA ; Omling, Pär LU ; Sundberg, M ; Tagerud, S and Montelius, Lars LU
- organization
- publishing date
- 2005
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- lab-on-a-chip, myosin, actin, molecular motors, nano-imprinting, factory-on-a-chip
- in
- Japanese Journal of Applied Physics
- volume
- 44
- issue
- 5A
- pages
- 3337 - 3340
- publisher
- IOP Publishing
- external identifiers
-
- wos:000231874900095
- scopus:22544477530
- ISSN
- 0021-4922
- DOI
- 10.1143/JJAP.44.3337
- language
- English
- LU publication?
- yes
- id
- 5ea9b477-c078-4f6c-9abb-d351880fb85f (old id 224139)
- date added to LUP
- 2016-04-01 11:52:38
- date last changed
- 2022-01-26 19:31:42
@article{5ea9b477-c078-4f6c-9abb-d351880fb85f, abstract = {{This work, for the first time, demonstrates that nano-imprinted samples, with 100 nm wide polymer lines, can act as guides for molecular motors consisting of motor proteins actin and myosin. The motor protein function was characterized using fluorescence microscopy and compared to actomyosin motility on non-structured nitrocellulose surfaces. Our results open for further use of the nano-imprint technique in the production of disposable chips for bio-nanotechnological applications and miniaturized biological test systems. We discuss how the nano-imprinted motor protein assay system may be optimized and also how it compares to previously tested assay systems involving low-resolution UV-lithography and low throughput but high-resolution electron beam lithography.}}, author = {{Bunk, Richard and Carlberg, Patrick and Mansson, A and Nicholls, IA and Omling, Pär and Sundberg, M and Tagerud, S and Montelius, Lars}}, issn = {{0021-4922}}, keywords = {{lab-on-a-chip; myosin; actin; molecular motors; nano-imprinting; factory-on-a-chip}}, language = {{eng}}, number = {{5A}}, pages = {{3337--3340}}, publisher = {{IOP Publishing}}, series = {{Japanese Journal of Applied Physics}}, title = {{Guiding molecular motors with nano-imprinted structures}}, url = {{http://dx.doi.org/10.1143/JJAP.44.3337}}, doi = {{10.1143/JJAP.44.3337}}, volume = {{44}}, year = {{2005}}, }