Sequence analysis, cloning and over-expression of an endoxylanase from the alkaliphilic Bacillus halodurans
(2005) In Biotechnology Letters 27(8). p.545-550- Abstract
- The BhMIR32 xyn11A gene, encoding an extracellular endoxylanase of potential interest in bio-bleaching applications, was amplified from Bacillus halodurans MIR32 genomic DNA. The protein encoded is an endo-1,4-beta-xylanase belonging to family 11 of glycosyl hydrolases. Its nucleotide sequence was analysed and the mature peptide was subcloned into pET22b(+) expression vector. The enzyme was over-expressed in a high density Escherichia coli culture as a soluble and active protein, and purified in a single step by immobilised metal ion affinity chromatography with a specific activity of 3073 IU mg(-1).
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/224637
- author
- Martinez, Alejandra LU ; Delgado, Osvaldo LU ; Baigori, M D and Sineriz, F
- organization
- publishing date
- 2005
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- purification, Bacillus halodurans MIR32, over-expression, sequence, xylanase
- in
- Biotechnology Letters
- volume
- 27
- issue
- 8
- pages
- 545 - 550
- publisher
- Springer
- external identifiers
-
- pmid:15973487
- wos:000231955300003
- scopus:20544471907
- ISSN
- 1573-6776
- DOI
- 10.1007/s10529-005-2879-2
- language
- English
- LU publication?
- yes
- id
- 2ecb9584-4441-4fe5-8fbc-1b0c1d91141a (old id 224637)
- date added to LUP
- 2016-04-01 16:11:25
- date last changed
- 2022-04-22 20:14:54
@article{2ecb9584-4441-4fe5-8fbc-1b0c1d91141a, abstract = {{The BhMIR32 xyn11A gene, encoding an extracellular endoxylanase of potential interest in bio-bleaching applications, was amplified from Bacillus halodurans MIR32 genomic DNA. The protein encoded is an endo-1,4-beta-xylanase belonging to family 11 of glycosyl hydrolases. Its nucleotide sequence was analysed and the mature peptide was subcloned into pET22b(+) expression vector. The enzyme was over-expressed in a high density Escherichia coli culture as a soluble and active protein, and purified in a single step by immobilised metal ion affinity chromatography with a specific activity of 3073 IU mg(-1).}}, author = {{Martinez, Alejandra and Delgado, Osvaldo and Baigori, M D and Sineriz, F}}, issn = {{1573-6776}}, keywords = {{purification; Bacillus halodurans MIR32; over-expression; sequence; xylanase}}, language = {{eng}}, number = {{8}}, pages = {{545--550}}, publisher = {{Springer}}, series = {{Biotechnology Letters}}, title = {{Sequence analysis, cloning and over-expression of an endoxylanase from the alkaliphilic Bacillus halodurans}}, url = {{http://dx.doi.org/10.1007/s10529-005-2879-2}}, doi = {{10.1007/s10529-005-2879-2}}, volume = {{27}}, year = {{2005}}, }