Formulation and in vitro/in vivo evaluation of combining DNA repair and immune enhancing nutritional supplements
(2005) In Phytomedicine 12(4). p.255-263- Abstract
- Combining nutritional supplements to achieve synergistic benefit is a common practice in the nutraceutical industry. However, establishing. added health benefit from a combination of natural ingredients is often assumed, untested and without regard to the principle of metabolic competition between the active components. Here, we report on the combination of a cat's claw water extract (C-Med-100, carboxy alkyl esters = active ingredients) + medicinal mushroom extracts (Cordyceps sinensis, Grifola blazei, Grifola frondosa, Trametes versicolor and Ganoderma lucidum, polysaccharides = active ingredients) + nicotinamide + zinc into a formulation designed to optimize different modes of immunostimulatory action, and yet that would avoid metabolic... (More)
- Combining nutritional supplements to achieve synergistic benefit is a common practice in the nutraceutical industry. However, establishing. added health benefit from a combination of natural ingredients is often assumed, untested and without regard to the principle of metabolic competition between the active components. Here, we report on the combination of a cat's claw water extract (C-Med-100, carboxy alkyl esters = active ingredients) + medicinal mushroom extracts (Cordyceps sinensis, Grifola blazei, Grifola frondosa, Trametes versicolor and Ganoderma lucidum, polysaccharides = active ingredients) + nicotinamide + zinc into a formulation designed to optimize different modes of immunostimulatory action, and yet that would avoid metabolic antioxidant competition yielding less than expected efficacious effects. Isobole curve analyses of these two active classes of ingredients determined by growth inhibition of HL-60 human leukemic cells in vitro confirmed they were indeed synergistic when in combination, and not metabolically competitive. Furthermore, an in vivo study showed significant health benefit for 14 subjects treated for 4 weeks with the unique C-Med-100/mushroom extract formulation in that they had reduced pain, reduced fatigue, weight loss and a reduced presence of DNA damage in peripheral blood assessed by (8-OH) guanine DNA ad, ducts and elevation in serum protein thiols. Because this broad-based panel of clinical parameters indicating clinical efficacy has never been demonstrated before for either of the active ingredients evaluated alone in humans, these data were taken as strong evidence that the combination of C-Med-100 + mushroom extracts + nicotinamide + zinc gave additive or synergistic effects to health benefit, and thus supported no efficacious limits from metabolic competition regarding this particular formulation. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/236401
- author
- Pero, Ronald LU ; Amiri, Amir LU ; Sheng, Yezhou LU ; Welther, M and Rich, M
- organization
- publishing date
- 2005
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- nutritional, Chinese mushroom extracts, cat's claw C-Med-100 extract, additive/synergistic efficacy, weight loss
- in
- Phytomedicine
- volume
- 12
- issue
- 4
- pages
- 255 - 263
- publisher
- Urban & Fischer Verlag
- external identifiers
-
- pmid:15898702
- wos:000229702400001
- scopus:16344375888
- ISSN
- 0944-7113
- DOI
- 10.1016/j.phymed.2004.01.008
- language
- English
- LU publication?
- yes
- id
- 18500fb4-cb4e-4014-953c-ea4f63bdefff (old id 236401)
- date added to LUP
- 2016-04-01 16:41:07
- date last changed
- 2022-04-15 06:16:49
@article{18500fb4-cb4e-4014-953c-ea4f63bdefff,
abstract = {{Combining nutritional supplements to achieve synergistic benefit is a common practice in the nutraceutical industry. However, establishing. added health benefit from a combination of natural ingredients is often assumed, untested and without regard to the principle of metabolic competition between the active components. Here, we report on the combination of a cat's claw water extract (C-Med-100, carboxy alkyl esters = active ingredients) + medicinal mushroom extracts (Cordyceps sinensis, Grifola blazei, Grifola frondosa, Trametes versicolor and Ganoderma lucidum, polysaccharides = active ingredients) + nicotinamide + zinc into a formulation designed to optimize different modes of immunostimulatory action, and yet that would avoid metabolic antioxidant competition yielding less than expected efficacious effects. Isobole curve analyses of these two active classes of ingredients determined by growth inhibition of HL-60 human leukemic cells in vitro confirmed they were indeed synergistic when in combination, and not metabolically competitive. Furthermore, an in vivo study showed significant health benefit for 14 subjects treated for 4 weeks with the unique C-Med-100/mushroom extract formulation in that they had reduced pain, reduced fatigue, weight loss and a reduced presence of DNA damage in peripheral blood assessed by (8-OH) guanine DNA ad, ducts and elevation in serum protein thiols. Because this broad-based panel of clinical parameters indicating clinical efficacy has never been demonstrated before for either of the active ingredients evaluated alone in humans, these data were taken as strong evidence that the combination of C-Med-100 + mushroom extracts + nicotinamide + zinc gave additive or synergistic effects to health benefit, and thus supported no efficacious limits from metabolic competition regarding this particular formulation.}},
author = {{Pero, Ronald and Amiri, Amir and Sheng, Yezhou and Welther, M and Rich, M}},
issn = {{0944-7113}},
keywords = {{nutritional; Chinese mushroom extracts; cat's claw C-Med-100 extract; additive/synergistic efficacy; weight loss}},
language = {{eng}},
number = {{4}},
pages = {{255--263}},
publisher = {{Urban & Fischer Verlag}},
series = {{Phytomedicine}},
title = {{Formulation and in vitro/in vivo evaluation of combining DNA repair and immune enhancing nutritional supplements}},
url = {{http://dx.doi.org/10.1016/j.phymed.2004.01.008}},
doi = {{10.1016/j.phymed.2004.01.008}},
volume = {{12}},
year = {{2005}},
}